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Immunomodulatory effects of human amniotic membrane-derived mesenchymal stem cells
Human amniotic membrane-derived mesenchymal stem cells (hAM-MSCs) are capable of differentiating into several lineages and possess immunomodulatory properties. In this study, we investigated the soluble factor-mediated immunomodulatory effects of hAM-MSCs. Mitogen-induced peripheral blood mononuclea...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Korean Society of Veterinary Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317453/ https://www.ncbi.nlm.nih.gov/pubmed/22437532 http://dx.doi.org/10.4142/jvs.2012.13.1.23 |
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author | Kang, Jung Won Koo, Hye Cheong Hwang, Sun Young Kang, Sung Keun Ra, Jeong Chan Lee, Moon Han Park, Yong Ho |
author_facet | Kang, Jung Won Koo, Hye Cheong Hwang, Sun Young Kang, Sung Keun Ra, Jeong Chan Lee, Moon Han Park, Yong Ho |
author_sort | Kang, Jung Won |
collection | PubMed |
description | Human amniotic membrane-derived mesenchymal stem cells (hAM-MSCs) are capable of differentiating into several lineages and possess immunomodulatory properties. In this study, we investigated the soluble factor-mediated immunomodulatory effects of hAM-MSCs. Mitogen-induced peripheral blood mononuclear cell (PBMC) proliferation was suppressed by hAM-MSCs in a dose-dependent manner as well as hAM-MSC culture supernatant. Moreover, interferon-gamma and interleukin (IL)-17 production significantly decreased from PBMC, whereas IL-10 from PBMCs and transforming growth factor beta (TGF-β) production from hAM-MSCs significantly increased in co-cultures of hAM-MSCs and PBMCs. Production of several MSC factors, including hepatocyte growth factor (HGF), TGF-β, prostaglandin E2 (PGE2), and indoleamine 2, 3 dioxygenase (IDO), increased significantly in hAM-MSCs co-cultured with PBMCs. These results indicate that the immunomodulatory effects of hAM-MSCs may be associated with soluble factors (TGF-β, HGF, PGE2, and IDO), suggesting that hAM-MSCs may have potential clinical use in regenerative medicine. |
format | Online Article Text |
id | pubmed-3317453 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Korean Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-33174532012-04-04 Immunomodulatory effects of human amniotic membrane-derived mesenchymal stem cells Kang, Jung Won Koo, Hye Cheong Hwang, Sun Young Kang, Sung Keun Ra, Jeong Chan Lee, Moon Han Park, Yong Ho J Vet Sci Original Article Human amniotic membrane-derived mesenchymal stem cells (hAM-MSCs) are capable of differentiating into several lineages and possess immunomodulatory properties. In this study, we investigated the soluble factor-mediated immunomodulatory effects of hAM-MSCs. Mitogen-induced peripheral blood mononuclear cell (PBMC) proliferation was suppressed by hAM-MSCs in a dose-dependent manner as well as hAM-MSC culture supernatant. Moreover, interferon-gamma and interleukin (IL)-17 production significantly decreased from PBMC, whereas IL-10 from PBMCs and transforming growth factor beta (TGF-β) production from hAM-MSCs significantly increased in co-cultures of hAM-MSCs and PBMCs. Production of several MSC factors, including hepatocyte growth factor (HGF), TGF-β, prostaglandin E2 (PGE2), and indoleamine 2, 3 dioxygenase (IDO), increased significantly in hAM-MSCs co-cultured with PBMCs. These results indicate that the immunomodulatory effects of hAM-MSCs may be associated with soluble factors (TGF-β, HGF, PGE2, and IDO), suggesting that hAM-MSCs may have potential clinical use in regenerative medicine. The Korean Society of Veterinary Science 2012-03 2012-03-20 /pmc/articles/PMC3317453/ /pubmed/22437532 http://dx.doi.org/10.4142/jvs.2012.13.1.23 Text en © 2012 The Korean Society of Veterinary Science. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kang, Jung Won Koo, Hye Cheong Hwang, Sun Young Kang, Sung Keun Ra, Jeong Chan Lee, Moon Han Park, Yong Ho Immunomodulatory effects of human amniotic membrane-derived mesenchymal stem cells |
title | Immunomodulatory effects of human amniotic membrane-derived mesenchymal stem cells |
title_full | Immunomodulatory effects of human amniotic membrane-derived mesenchymal stem cells |
title_fullStr | Immunomodulatory effects of human amniotic membrane-derived mesenchymal stem cells |
title_full_unstemmed | Immunomodulatory effects of human amniotic membrane-derived mesenchymal stem cells |
title_short | Immunomodulatory effects of human amniotic membrane-derived mesenchymal stem cells |
title_sort | immunomodulatory effects of human amniotic membrane-derived mesenchymal stem cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317453/ https://www.ncbi.nlm.nih.gov/pubmed/22437532 http://dx.doi.org/10.4142/jvs.2012.13.1.23 |
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