Evaluation and comparison of native and recombinant LipL21 protein-based ELISAs for diagnosis of bovine leptospirosis

A 21-kDa leptospiral lipoprotein (LipL21) was evaluated for its diagnostic potential to detect bovine leptospirosis by ELISA. Both native LipL21 (nLipL21) and recombinant LipL21 (rLipL21) proteins were tested and compared regarding diagnostic efficiency, and no statistically significant difference w...

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Detalles Bibliográficos
Autores principales: Joseph, Siju, Thomas, Naicy, Thangapandian, E., Singh, Vijendra P, Verma, Rishendra, Srivastava, S. K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2012
Materias:
Short Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317464/
https://www.ncbi.nlm.nih.gov/pubmed/22437542
http://dx.doi.org/10.4142/jvs.2012.13.1.99
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author Joseph, Siju
Thomas, Naicy
Thangapandian, E.
Singh, Vijendra P
Verma, Rishendra
Srivastava, S. K.
author_facet Joseph, Siju
Thomas, Naicy
Thangapandian, E.
Singh, Vijendra P
Verma, Rishendra
Srivastava, S. K.
author_sort Joseph, Siju
collection PubMed
description A 21-kDa leptospiral lipoprotein (LipL21) was evaluated for its diagnostic potential to detect bovine leptospirosis by ELISA. Both native LipL21 (nLipL21) and recombinant LipL21 (rLipL21) proteins were tested and compared regarding diagnostic efficiency, and no statistically significant difference was observed. The sensitivity of rLipL21 ELISA for 62 microscopic agglutination test (MAT) positive sera was 100% and the specificity with 378 MAT negative sera was 97.09%. Thus, rLipL21 protein-based ELISA could be used as an alternative to MAT for the diagnosis of bovine leptospirosis.
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spelling pubmed-33174642012-04-04 Evaluation and comparison of native and recombinant LipL21 protein-based ELISAs for diagnosis of bovine leptospirosis Joseph, Siju Thomas, Naicy Thangapandian, E. Singh, Vijendra P Verma, Rishendra Srivastava, S. K. J Vet Sci Short Communication A 21-kDa leptospiral lipoprotein (LipL21) was evaluated for its diagnostic potential to detect bovine leptospirosis by ELISA. Both native LipL21 (nLipL21) and recombinant LipL21 (rLipL21) proteins were tested and compared regarding diagnostic efficiency, and no statistically significant difference was observed. The sensitivity of rLipL21 ELISA for 62 microscopic agglutination test (MAT) positive sera was 100% and the specificity with 378 MAT negative sera was 97.09%. Thus, rLipL21 protein-based ELISA could be used as an alternative to MAT for the diagnosis of bovine leptospirosis. The Korean Society of Veterinary Science 2012-03 2012-03-20 /pmc/articles/PMC3317464/ /pubmed/22437542 http://dx.doi.org/10.4142/jvs.2012.13.1.99 Text en © 2012 The Korean Society of Veterinary Science. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Short Communication
Joseph, Siju
Thomas, Naicy
Thangapandian, E.
Singh, Vijendra P
Verma, Rishendra
Srivastava, S. K.
Evaluation and comparison of native and recombinant LipL21 protein-based ELISAs for diagnosis of bovine leptospirosis
title Evaluation and comparison of native and recombinant LipL21 protein-based ELISAs for diagnosis of bovine leptospirosis
title_full Evaluation and comparison of native and recombinant LipL21 protein-based ELISAs for diagnosis of bovine leptospirosis
title_fullStr Evaluation and comparison of native and recombinant LipL21 protein-based ELISAs for diagnosis of bovine leptospirosis
title_full_unstemmed Evaluation and comparison of native and recombinant LipL21 protein-based ELISAs for diagnosis of bovine leptospirosis
title_short Evaluation and comparison of native and recombinant LipL21 protein-based ELISAs for diagnosis of bovine leptospirosis
title_sort evaluation and comparison of native and recombinant lipl21 protein-based elisas for diagnosis of bovine leptospirosis
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317464/
https://www.ncbi.nlm.nih.gov/pubmed/22437542
http://dx.doi.org/10.4142/jvs.2012.13.1.99
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