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Significant Correlation between Chromosomal Aberration and Nuclear Morphology in Urothelial Carcinoma
We aimed to identify whether there is any correlation between chromosomal/genetic changes, nuclear morphology and the histological grade of urothelial carcinomas of the urinary bladder. Morphometry and multicolour fluorescence in situ hybridisation (FISH) techniques were applied to 250 cells in five...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japan Society of Histochemistry and Cytochemistry
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317491/ https://www.ncbi.nlm.nih.gov/pubmed/22489102 http://dx.doi.org/10.1267/ahc.11048 |
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author | Shuto, Masayo Seyama, Atsushi Gotoh, Yoshiya Kamada, Kouichi Nakamura, Masaru Warigaya, Kenji Watanabe, Hiroshi Ueno, Munehisa Shimizu, Michio Fukuda, Toshio Murata, Shin-ichi |
author_facet | Shuto, Masayo Seyama, Atsushi Gotoh, Yoshiya Kamada, Kouichi Nakamura, Masaru Warigaya, Kenji Watanabe, Hiroshi Ueno, Munehisa Shimizu, Michio Fukuda, Toshio Murata, Shin-ichi |
author_sort | Shuto, Masayo |
collection | PubMed |
description | We aimed to identify whether there is any correlation between chromosomal/genetic changes, nuclear morphology and the histological grade of urothelial carcinomas of the urinary bladder. Morphometry and multicolour fluorescence in situ hybridisation (FISH) techniques were applied to 250 cells in five low-grade cases and 350 cells in seven high-grade cases of urothelial carcinoma. Compared with low-grade carcinomas, most high-grade cases showed larger and more variable nuclear size, more frequent polysomy of centromere enumeration probes (CEPs) 3, 7 and 17, and the loss of the 9p21 locus. The number of CEP signals in cells was increased as the nuclear area of the cells became larger. Cells with gains in two or more types of CEP had significantly larger nuclei than cells with normal FISH signal patterns. In conclusion, the present study indicates that there was a correlation between nuclear morphology and chromosomal/genetic changes which were related to histological grading. Thus, we show that differences in the chromosomal/genetic aberrations present in low- and high-grade tumours can affect not only nuclear morphology but also the histopathological and clinical behaviour of urothelial carcinomas. |
format | Online Article Text |
id | pubmed-3317491 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Japan Society of Histochemistry and Cytochemistry |
record_format | MEDLINE/PubMed |
spelling | pubmed-33174912012-04-09 Significant Correlation between Chromosomal Aberration and Nuclear Morphology in Urothelial Carcinoma Shuto, Masayo Seyama, Atsushi Gotoh, Yoshiya Kamada, Kouichi Nakamura, Masaru Warigaya, Kenji Watanabe, Hiroshi Ueno, Munehisa Shimizu, Michio Fukuda, Toshio Murata, Shin-ichi Acta Histochem Cytochem Regular Article We aimed to identify whether there is any correlation between chromosomal/genetic changes, nuclear morphology and the histological grade of urothelial carcinomas of the urinary bladder. Morphometry and multicolour fluorescence in situ hybridisation (FISH) techniques were applied to 250 cells in five low-grade cases and 350 cells in seven high-grade cases of urothelial carcinoma. Compared with low-grade carcinomas, most high-grade cases showed larger and more variable nuclear size, more frequent polysomy of centromere enumeration probes (CEPs) 3, 7 and 17, and the loss of the 9p21 locus. The number of CEP signals in cells was increased as the nuclear area of the cells became larger. Cells with gains in two or more types of CEP had significantly larger nuclei than cells with normal FISH signal patterns. In conclusion, the present study indicates that there was a correlation between nuclear morphology and chromosomal/genetic changes which were related to histological grading. Thus, we show that differences in the chromosomal/genetic aberrations present in low- and high-grade tumours can affect not only nuclear morphology but also the histopathological and clinical behaviour of urothelial carcinomas. Japan Society of Histochemistry and Cytochemistry 2012-02-29 2012-01-24 /pmc/articles/PMC3317491/ /pubmed/22489102 http://dx.doi.org/10.1267/ahc.11048 Text en © 2012 The Japan Society of Histochemistry and Cytochemistry This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Regular Article Shuto, Masayo Seyama, Atsushi Gotoh, Yoshiya Kamada, Kouichi Nakamura, Masaru Warigaya, Kenji Watanabe, Hiroshi Ueno, Munehisa Shimizu, Michio Fukuda, Toshio Murata, Shin-ichi Significant Correlation between Chromosomal Aberration and Nuclear Morphology in Urothelial Carcinoma |
title | Significant Correlation between Chromosomal Aberration and Nuclear Morphology in Urothelial Carcinoma |
title_full | Significant Correlation between Chromosomal Aberration and Nuclear Morphology in Urothelial Carcinoma |
title_fullStr | Significant Correlation between Chromosomal Aberration and Nuclear Morphology in Urothelial Carcinoma |
title_full_unstemmed | Significant Correlation between Chromosomal Aberration and Nuclear Morphology in Urothelial Carcinoma |
title_short | Significant Correlation between Chromosomal Aberration and Nuclear Morphology in Urothelial Carcinoma |
title_sort | significant correlation between chromosomal aberration and nuclear morphology in urothelial carcinoma |
topic | Regular Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317491/ https://www.ncbi.nlm.nih.gov/pubmed/22489102 http://dx.doi.org/10.1267/ahc.11048 |
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