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Molecular Detection and Characterization of Goat Isolate of Taenia hydatigena in Turkey
The aim of this study was to provide molecular detection and characterization of the goat isolate of Taenia hydatigena from Ankara province of Turkey. For this purpose, PCR amplification of small subunit ribosomal RNA (rrnS) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Scientific World Journal
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317571/ https://www.ncbi.nlm.nih.gov/pubmed/22500144 http://dx.doi.org/10.1100/2012/962732 |
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author | Utuk, Armagan Erdem Piskin, Fatma Cigdem |
author_facet | Utuk, Armagan Erdem Piskin, Fatma Cigdem |
author_sort | Utuk, Armagan Erdem |
collection | PubMed |
description | The aim of this study was to provide molecular detection and characterization of the goat isolate of Taenia hydatigena from Ankara province of Turkey. For this purpose, PCR amplification of small subunit ribosomal RNA (rrnS) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) genes were performed in a one-month-old dead goat. According to rrnS-PCR results, parasites were identified as Taenia spp., and partial sequence of mt-CO1 gene was corresponding to T. hydatigena. At the end of the study, we concluded that molecular tools can be used to define species of parasites in cases where the key morphologic features cannot be detected. Nucleotide sequence data of Turkish goat isolate of T. hydatigena was submitted to GenBank for other researchers interested in this subject. By this study, molecular detection and characterization of T. hydatigena was done for the first time in Turkey. |
format | Online Article Text |
id | pubmed-3317571 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | The Scientific World Journal |
record_format | MEDLINE/PubMed |
spelling | pubmed-33175712012-04-12 Molecular Detection and Characterization of Goat Isolate of Taenia hydatigena in Turkey Utuk, Armagan Erdem Piskin, Fatma Cigdem ScientificWorldJournal Research Article The aim of this study was to provide molecular detection and characterization of the goat isolate of Taenia hydatigena from Ankara province of Turkey. For this purpose, PCR amplification of small subunit ribosomal RNA (rrnS) and partial sequencing of mitochondrial cytochrome c oxidase subunit 1 (mt-CO1) genes were performed in a one-month-old dead goat. According to rrnS-PCR results, parasites were identified as Taenia spp., and partial sequence of mt-CO1 gene was corresponding to T. hydatigena. At the end of the study, we concluded that molecular tools can be used to define species of parasites in cases where the key morphologic features cannot be detected. Nucleotide sequence data of Turkish goat isolate of T. hydatigena was submitted to GenBank for other researchers interested in this subject. By this study, molecular detection and characterization of T. hydatigena was done for the first time in Turkey. The Scientific World Journal 2012-03-12 /pmc/articles/PMC3317571/ /pubmed/22500144 http://dx.doi.org/10.1100/2012/962732 Text en Copyright © 2012 A. E. Utuk and F. C. Piskin. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Utuk, Armagan Erdem Piskin, Fatma Cigdem Molecular Detection and Characterization of Goat Isolate of Taenia hydatigena in Turkey |
title | Molecular Detection and Characterization of Goat Isolate of Taenia hydatigena in Turkey |
title_full | Molecular Detection and Characterization of Goat Isolate of Taenia hydatigena in Turkey |
title_fullStr | Molecular Detection and Characterization of Goat Isolate of Taenia hydatigena in Turkey |
title_full_unstemmed | Molecular Detection and Characterization of Goat Isolate of Taenia hydatigena in Turkey |
title_short | Molecular Detection and Characterization of Goat Isolate of Taenia hydatigena in Turkey |
title_sort | molecular detection and characterization of goat isolate of taenia hydatigena in turkey |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317571/ https://www.ncbi.nlm.nih.gov/pubmed/22500144 http://dx.doi.org/10.1100/2012/962732 |
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