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Skip the Alignment: Degenerate, Multiplex Primer and Probe Design Using K-mer Matching Instead of Alignments

PriMux is a new software package for selecting multiplex compatible, degenerate primers and probes to detect diverse targets such as viruses. It requires no multiple sequence alignment, instead applying k-mer algorithms, hence it scales well for large target sets and saves user effort from curating...

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Detalles Bibliográficos
Autores principales: Hysom, David A., Naraghi-Arani, Pejman, Elsheikh, Maher, Carrillo, A. Celena, Williams, Peter L., Gardner, Shea N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317645/
https://www.ncbi.nlm.nih.gov/pubmed/22485178
http://dx.doi.org/10.1371/journal.pone.0034560

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