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Skip the Alignment: Degenerate, Multiplex Primer and Probe Design Using K-mer Matching Instead of Alignments
PriMux is a new software package for selecting multiplex compatible, degenerate primers and probes to detect diverse targets such as viruses. It requires no multiple sequence alignment, instead applying k-mer algorithms, hence it scales well for large target sets and saves user effort from curating...
Autores principales: | Hysom, David A., Naraghi-Arani, Pejman, Elsheikh, Maher, Carrillo, A. Celena, Williams, Peter L., Gardner, Shea N. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317645/ https://www.ncbi.nlm.nih.gov/pubmed/22485178 http://dx.doi.org/10.1371/journal.pone.0034560 |
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