Molecular Mechanisms of (R,R)ZX-5 on NO Synthesis and Its Anti-Angiogenic Effect

(R,R)ZX-5 is a NO regulatory compound, which could significantly increase choroidal blood flow in New Zealand rabbit. The aim of this paper is to investigate the molecular mechanism of (R,R)ZX-5 promoting NO production. Besides this, we also investigated the antiangiogenic activity of (R,R)ZX-5. Ana...

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Detalles Bibliográficos
Autores principales: Pan, Li, Hu, Jia-Liang, Wang, Wen-Jing, Zhang, Xiao-Juan, Wei, Jin, Liu, Zhen-Dong, Zhang, Yi-Hua, Xu, Han-Mei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2012
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317683/
https://www.ncbi.nlm.nih.gov/pubmed/22489120
http://dx.doi.org/10.3390/ijms13032717
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author Pan, Li
Hu, Jia-Liang
Wang, Wen-Jing
Zhang, Xiao-Juan
Wei, Jin
Liu, Zhen-Dong
Zhang, Yi-Hua
Xu, Han-Mei
author_facet Pan, Li
Hu, Jia-Liang
Wang, Wen-Jing
Zhang, Xiao-Juan
Wei, Jin
Liu, Zhen-Dong
Zhang, Yi-Hua
Xu, Han-Mei
author_sort Pan, Li
collection PubMed
description (R,R)ZX-5 is a NO regulatory compound, which could significantly increase choroidal blood flow in New Zealand rabbit. The aim of this paper is to investigate the molecular mechanism of (R,R)ZX-5 promoting NO production. Besides this, we also investigated the antiangiogenic activity of (R,R)ZX-5. Analysis of Western blot showed that (R,R)ZX-5 up-regulated the expression of Akt, p-Akt (Thr473), eNOS and p-eNOS (Ser1177), down-regulated the expression of Cyclin D1 in human retinal endothelial cells and escalated the intracellular free Ca(2+) concentration. Additionally, (R,R)ZX-5 inhibited the growth of blood vessels in the chick chorioallantoic membrane model. It is concluded that (R,R)ZX-5 promotes choroidal blood flow through PI3K/Akt-eNOS and Akt-Ca(2+)-eNOS pathways. Additionally, (R,R)ZX-5 can inhibit angiogenesis.
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spelling pubmed-33176832012-04-09 Molecular Mechanisms of (R,R)ZX-5 on NO Synthesis and Its Anti-Angiogenic Effect Pan, Li Hu, Jia-Liang Wang, Wen-Jing Zhang, Xiao-Juan Wei, Jin Liu, Zhen-Dong Zhang, Yi-Hua Xu, Han-Mei Int J Mol Sci Article (R,R)ZX-5 is a NO regulatory compound, which could significantly increase choroidal blood flow in New Zealand rabbit. The aim of this paper is to investigate the molecular mechanism of (R,R)ZX-5 promoting NO production. Besides this, we also investigated the antiangiogenic activity of (R,R)ZX-5. Analysis of Western blot showed that (R,R)ZX-5 up-regulated the expression of Akt, p-Akt (Thr473), eNOS and p-eNOS (Ser1177), down-regulated the expression of Cyclin D1 in human retinal endothelial cells and escalated the intracellular free Ca(2+) concentration. Additionally, (R,R)ZX-5 inhibited the growth of blood vessels in the chick chorioallantoic membrane model. It is concluded that (R,R)ZX-5 promotes choroidal blood flow through PI3K/Akt-eNOS and Akt-Ca(2+)-eNOS pathways. Additionally, (R,R)ZX-5 can inhibit angiogenesis. Molecular Diversity Preservation International (MDPI) 2012-02-29 /pmc/articles/PMC3317683/ /pubmed/22489120 http://dx.doi.org/10.3390/ijms13032717 Text en © 2012 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Pan, Li
Hu, Jia-Liang
Wang, Wen-Jing
Zhang, Xiao-Juan
Wei, Jin
Liu, Zhen-Dong
Zhang, Yi-Hua
Xu, Han-Mei
Molecular Mechanisms of (R,R)ZX-5 on NO Synthesis and Its Anti-Angiogenic Effect
title Molecular Mechanisms of (R,R)ZX-5 on NO Synthesis and Its Anti-Angiogenic Effect
title_full Molecular Mechanisms of (R,R)ZX-5 on NO Synthesis and Its Anti-Angiogenic Effect
title_fullStr Molecular Mechanisms of (R,R)ZX-5 on NO Synthesis and Its Anti-Angiogenic Effect
title_full_unstemmed Molecular Mechanisms of (R,R)ZX-5 on NO Synthesis and Its Anti-Angiogenic Effect
title_short Molecular Mechanisms of (R,R)ZX-5 on NO Synthesis and Its Anti-Angiogenic Effect
title_sort molecular mechanisms of (r,r)zx-5 on no synthesis and its anti-angiogenic effect
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317683/
https://www.ncbi.nlm.nih.gov/pubmed/22489120
http://dx.doi.org/10.3390/ijms13032717
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