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Nuclear translation visualized by ribosome-bound nascent chain puromycylation

Whether protein translation occurs in the nucleus is contentious. To address this question, we developed the ribopuromycylation method (RPM), which visualizes translation in cells via standard immunofluorescence microscopy. The RPM is based on ribosome-catalyzed puromycylation of nascent chains immo...

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Detalles Bibliográficos
Autores principales:	David, Alexandre, Dolan, Brian P., Hickman, Heather D., Knowlton, Jonathan J., Clavarino, Giovanna, Pierre, Philippe, Bennink, Jack R., Yewdell, Jonathan W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	The Rockefeller University Press 2012
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317795/ https://www.ncbi.nlm.nih.gov/pubmed/22472439 http://dx.doi.org/10.1083/jcb.201112145

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