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Multivariate proteomic profiling identifies novel accessory proteins of coated vesicles
Despite recent advances in mass spectrometry, proteomic characterization of transport vesicles remains challenging. Here, we describe a multivariate proteomics approach to analyzing clathrin-coated vesicles (CCVs) from HeLa cells. siRNA knockdown of coat components and different fractionation protoc...
Autores principales: | Borner, Georg H.H., Antrobus, Robin, Hirst, Jennifer, Bhumbra, Gary S., Kozik, Patrycja, Jackson, Lauren P., Sahlender, Daniela A., Robinson, Margaret S. |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3317806/ https://www.ncbi.nlm.nih.gov/pubmed/22472443 http://dx.doi.org/10.1083/jcb.201111049 |
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