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Prednisolone exerts exquisite inhibitory properties on platelet functions
We have previously reported presence of the glucocorticoid (GC) receptor (GR) alpha on blood platelets, and its ability to modulate platelet aggregation when activated by the synthetic GC prednisolone (Pred). In the present study we investigated the effects of Pred on broader aspects of platelet fun...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3320711/ https://www.ncbi.nlm.nih.gov/pubmed/22366284 http://dx.doi.org/10.1016/j.bcp.2012.02.006 |
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author | Liverani, Elisabetta Banerjee, Sreemoti Roberts, Wayne Naseem, Khalid M. Perretti, Mauro |
author_facet | Liverani, Elisabetta Banerjee, Sreemoti Roberts, Wayne Naseem, Khalid M. Perretti, Mauro |
author_sort | Liverani, Elisabetta |
collection | PubMed |
description | We have previously reported presence of the glucocorticoid (GC) receptor (GR) alpha on blood platelets, and its ability to modulate platelet aggregation when activated by the synthetic GC prednisolone (Pred). In the present study we investigated the effects of Pred on broader aspects of platelet functions to unveil novel non-genomic actions on this cell type. Using whole blood assay we demonstrated that Pred was the only GC able to inhibit platelet aggregation and platelet–monocyte interactions. This latter effect was due to regulation of platelets, not monocytes. We next examined the effects of Pred on physiological actions of platelets, observing inhibition of platelet adhesion and spreading on collagen under static conditions. Moreover Pred inhibited thrombus formation under flow, suggesting potential important effects in haemostasis and thrombosis. Pred was unable to regulate platelet reactivity under conditions where the effects of platelet-derived ADP and TxA(2) were blocked, suggesting that the GC targeted the activation-dependent component of the adhesion and aggregation response. The effects of Pred were not mediated through cyclic nucleotide signaling, but rather seemed to evolve around selective regulation of P2Y(12) ADP receptor signaling, intimating a novel mode of action. This study details the actions of Pred on platelets unveiling novel properties which could be relevant for this GC in controlling unwanted vascular and thrombotic diseases. |
format | Online Article Text |
id | pubmed-3320711 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Elsevier Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-33207112012-05-15 Prednisolone exerts exquisite inhibitory properties on platelet functions Liverani, Elisabetta Banerjee, Sreemoti Roberts, Wayne Naseem, Khalid M. Perretti, Mauro Biochem Pharmacol Article We have previously reported presence of the glucocorticoid (GC) receptor (GR) alpha on blood platelets, and its ability to modulate platelet aggregation when activated by the synthetic GC prednisolone (Pred). In the present study we investigated the effects of Pred on broader aspects of platelet functions to unveil novel non-genomic actions on this cell type. Using whole blood assay we demonstrated that Pred was the only GC able to inhibit platelet aggregation and platelet–monocyte interactions. This latter effect was due to regulation of platelets, not monocytes. We next examined the effects of Pred on physiological actions of platelets, observing inhibition of platelet adhesion and spreading on collagen under static conditions. Moreover Pred inhibited thrombus formation under flow, suggesting potential important effects in haemostasis and thrombosis. Pred was unable to regulate platelet reactivity under conditions where the effects of platelet-derived ADP and TxA(2) were blocked, suggesting that the GC targeted the activation-dependent component of the adhesion and aggregation response. The effects of Pred were not mediated through cyclic nucleotide signaling, but rather seemed to evolve around selective regulation of P2Y(12) ADP receptor signaling, intimating a novel mode of action. This study details the actions of Pred on platelets unveiling novel properties which could be relevant for this GC in controlling unwanted vascular and thrombotic diseases. Elsevier Science 2012-05-15 /pmc/articles/PMC3320711/ /pubmed/22366284 http://dx.doi.org/10.1016/j.bcp.2012.02.006 Text en © 2012 Elsevier Inc. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license |
spellingShingle | Article Liverani, Elisabetta Banerjee, Sreemoti Roberts, Wayne Naseem, Khalid M. Perretti, Mauro Prednisolone exerts exquisite inhibitory properties on platelet functions |
title | Prednisolone exerts exquisite inhibitory properties on platelet functions |
title_full | Prednisolone exerts exquisite inhibitory properties on platelet functions |
title_fullStr | Prednisolone exerts exquisite inhibitory properties on platelet functions |
title_full_unstemmed | Prednisolone exerts exquisite inhibitory properties on platelet functions |
title_short | Prednisolone exerts exquisite inhibitory properties on platelet functions |
title_sort | prednisolone exerts exquisite inhibitory properties on platelet functions |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3320711/ https://www.ncbi.nlm.nih.gov/pubmed/22366284 http://dx.doi.org/10.1016/j.bcp.2012.02.006 |
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