Phylogenetic Analysis of Enterohemorrhagic Escherichia coli O157, Germany, 1987–2008

Multilocus variable number tandem repeat analysis (MLVA) is a subtyping technique for characterizing human pathogenic bacteria such as enterohemorrhagic Escherichia coli (EHEC) O157. We determined the phylogeny of 202 epidemiologically unrelated EHEC O157:H7/H(–) clinical isolates through 8 MLVA loc...

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Detalles Bibliográficos
Autores principales: Jenke, Christian, Harmsen, Dag, Weniger, Thomas, Rothgänger, Jörg, Hyytiä-Trees, Eija, Bielaszewska, Martina, Karch, Helge, Mellmann, Alexander
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2010
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3321953/
https://www.ncbi.nlm.nih.gov/pubmed/20350374
http://dx.doi.org/10.3201/eid1604.091361
Descripción
Sumario:Multilocus variable number tandem repeat analysis (MLVA) is a subtyping technique for characterizing human pathogenic bacteria such as enterohemorrhagic Escherichia coli (EHEC) O157. We determined the phylogeny of 202 epidemiologically unrelated EHEC O157:H7/H(–) clinical isolates through 8 MLVA loci obtained in Germany during 1987–2008. Biodiversity in the loci ranged from 0.66 to 0.90. Four of 8 loci showed null alleles and a frequency <44.1%. These loci were distributed among 48.5% of all strains. Overall, 141 MLVA profiles were identified. Phylogenetic analysis assigned 67.3% of the strains to 19 MLVA clusters. Specific MLVA profiles with an evolutionary persistence were identified, particularly within sorbitol-fermenting EHEC O157:H(–).These pathogens belonged to the same MLVA cluster. Our findings indicate successful persistence of this clone.

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