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Conservation of Tubulin-Binding Sequences in TRPV1 throughout Evolution

BACKGROUND: Transient Receptor Potential Vanilloid sub type 1 (TRPV1), commonly known as capsaicin receptor can detect multiple stimuli ranging from noxious compounds, low pH, temperature as well as electromagnetic wave at different ranges. In addition, this receptor is involved in multiple physiolo...

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Autores principales: Sardar, Puspendu, Kumar, Abhishek, Bhandari, Anita, Goswami, Chandan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3322131/
https://www.ncbi.nlm.nih.gov/pubmed/22496727
http://dx.doi.org/10.1371/journal.pone.0031448
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author Sardar, Puspendu
Kumar, Abhishek
Bhandari, Anita
Goswami, Chandan
author_facet Sardar, Puspendu
Kumar, Abhishek
Bhandari, Anita
Goswami, Chandan
author_sort Sardar, Puspendu
collection PubMed
description BACKGROUND: Transient Receptor Potential Vanilloid sub type 1 (TRPV1), commonly known as capsaicin receptor can detect multiple stimuli ranging from noxious compounds, low pH, temperature as well as electromagnetic wave at different ranges. In addition, this receptor is involved in multiple physiological and sensory processes. Therefore, functions of TRPV1 have direct influences on adaptation and further evolution also. Availability of various eukaryotic genomic sequences in public domain facilitates us in studying the molecular evolution of TRPV1 protein and the respective conservation of certain domains, motifs and interacting regions that are functionally important. METHODOLOGY AND PRINCIPAL FINDINGS: Using statistical and bioinformatics tools, our analysis reveals that TRPV1 has evolved about ∼420 million years ago (MYA). Our analysis reveals that specific regions, domains and motifs of TRPV1 has gone through different selection pressure and thus have different levels of conservation. We found that among all, TRP box is the most conserved and thus have functional significance. Our results also indicate that the tubulin binding sequences (TBS) have evolutionary significance as these stretch sequences are more conserved than many other essential regions of TRPV1. The overall distribution of positively charged residues within the TBS motifs is conserved throughout evolution. In silico analysis reveals that the TBS-1 and TBS-2 of TRPV1 can form helical structures and may play important role in TRPV1 function. CONCLUSIONS AND SIGNIFICANCE: Our analysis identifies the regions of TRPV1, which are important for structure – function relationship. This analysis indicates that tubulin binding sequence-1 (TBS-1) near the TRP-box forms a potential helix and the tubulin interactions with TRPV1 via TBS-1 have evolutionary significance. This interaction may be required for the proper channel function and regulation and may also have significance in the context of Taxol®-induced neuropathy.
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spelling pubmed-33221312012-04-11 Conservation of Tubulin-Binding Sequences in TRPV1 throughout Evolution Sardar, Puspendu Kumar, Abhishek Bhandari, Anita Goswami, Chandan PLoS One Research Article BACKGROUND: Transient Receptor Potential Vanilloid sub type 1 (TRPV1), commonly known as capsaicin receptor can detect multiple stimuli ranging from noxious compounds, low pH, temperature as well as electromagnetic wave at different ranges. In addition, this receptor is involved in multiple physiological and sensory processes. Therefore, functions of TRPV1 have direct influences on adaptation and further evolution also. Availability of various eukaryotic genomic sequences in public domain facilitates us in studying the molecular evolution of TRPV1 protein and the respective conservation of certain domains, motifs and interacting regions that are functionally important. METHODOLOGY AND PRINCIPAL FINDINGS: Using statistical and bioinformatics tools, our analysis reveals that TRPV1 has evolved about ∼420 million years ago (MYA). Our analysis reveals that specific regions, domains and motifs of TRPV1 has gone through different selection pressure and thus have different levels of conservation. We found that among all, TRP box is the most conserved and thus have functional significance. Our results also indicate that the tubulin binding sequences (TBS) have evolutionary significance as these stretch sequences are more conserved than many other essential regions of TRPV1. The overall distribution of positively charged residues within the TBS motifs is conserved throughout evolution. In silico analysis reveals that the TBS-1 and TBS-2 of TRPV1 can form helical structures and may play important role in TRPV1 function. CONCLUSIONS AND SIGNIFICANCE: Our analysis identifies the regions of TRPV1, which are important for structure – function relationship. This analysis indicates that tubulin binding sequence-1 (TBS-1) near the TRP-box forms a potential helix and the tubulin interactions with TRPV1 via TBS-1 have evolutionary significance. This interaction may be required for the proper channel function and regulation and may also have significance in the context of Taxol®-induced neuropathy. Public Library of Science 2012-04-09 /pmc/articles/PMC3322131/ /pubmed/22496727 http://dx.doi.org/10.1371/journal.pone.0031448 Text en Sardar et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Sardar, Puspendu
Kumar, Abhishek
Bhandari, Anita
Goswami, Chandan
Conservation of Tubulin-Binding Sequences in TRPV1 throughout Evolution
title Conservation of Tubulin-Binding Sequences in TRPV1 throughout Evolution
title_full Conservation of Tubulin-Binding Sequences in TRPV1 throughout Evolution
title_fullStr Conservation of Tubulin-Binding Sequences in TRPV1 throughout Evolution
title_full_unstemmed Conservation of Tubulin-Binding Sequences in TRPV1 throughout Evolution
title_short Conservation of Tubulin-Binding Sequences in TRPV1 throughout Evolution
title_sort conservation of tubulin-binding sequences in trpv1 throughout evolution
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3322131/
https://www.ncbi.nlm.nih.gov/pubmed/22496727
http://dx.doi.org/10.1371/journal.pone.0031448
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