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Genetic Characterization of Streptococcus iniae in Diseased Farmed Rainbow Trout (Onchorhynchus mykiss) in Iran

Genetic characterization of strains of Streptococcus iniae recovered from morbidity and mortality of farmed rainbow trout in different provinces of Iran were studied. The Gram-positive cocci isolates were obtained from the kidney tissues of diseased rainbow trout on blood agar at 25°C for 72 h. The...

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Autores principales: Erfanmanesh, A., Soltani, M., Pirali, E., Mohammadian, S., Taherimirghaed, A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Scientific World Journal 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3322394/
https://www.ncbi.nlm.nih.gov/pubmed/22550467
http://dx.doi.org/10.1100/2012/594073
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author Erfanmanesh, A.
Soltani, M.
Pirali, E.
Mohammadian, S.
Taherimirghaed, A.
author_facet Erfanmanesh, A.
Soltani, M.
Pirali, E.
Mohammadian, S.
Taherimirghaed, A.
author_sort Erfanmanesh, A.
collection PubMed
description Genetic characterization of strains of Streptococcus iniae recovered from morbidity and mortality of farmed rainbow trout in different provinces of Iran were studied. The Gram-positive cocci isolates were obtained from the kidney tissues of diseased rainbow trout on blood agar at 25°C for 72 h. The grown bacteria were then characterized using biochemical and molecular works. The identified 26 isolates of S. iniae producing a 513 bp in PCR procedure were then compared using random amplified polymorphic DNA (RAPD) analysis using 9 random primers. The phylogenetic tree of the RAPD product using UPMGA software included these strains in one genetic group but into two clusters. The results of this study show that S. iniae strains from the diseased rainbow trout in the north part of Iran are genetically similar to those strains in the south and west parts of the country.
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spelling pubmed-33223942012-05-01 Genetic Characterization of Streptococcus iniae in Diseased Farmed Rainbow Trout (Onchorhynchus mykiss) in Iran Erfanmanesh, A. Soltani, M. Pirali, E. Mohammadian, S. Taherimirghaed, A. ScientificWorldJournal Research Article Genetic characterization of strains of Streptococcus iniae recovered from morbidity and mortality of farmed rainbow trout in different provinces of Iran were studied. The Gram-positive cocci isolates were obtained from the kidney tissues of diseased rainbow trout on blood agar at 25°C for 72 h. The grown bacteria were then characterized using biochemical and molecular works. The identified 26 isolates of S. iniae producing a 513 bp in PCR procedure were then compared using random amplified polymorphic DNA (RAPD) analysis using 9 random primers. The phylogenetic tree of the RAPD product using UPMGA software included these strains in one genetic group but into two clusters. The results of this study show that S. iniae strains from the diseased rainbow trout in the north part of Iran are genetically similar to those strains in the south and west parts of the country. The Scientific World Journal 2012-04-01 /pmc/articles/PMC3322394/ /pubmed/22550467 http://dx.doi.org/10.1100/2012/594073 Text en Copyright © 2012 A. Erfanmanesh et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Erfanmanesh, A.
Soltani, M.
Pirali, E.
Mohammadian, S.
Taherimirghaed, A.
Genetic Characterization of Streptococcus iniae in Diseased Farmed Rainbow Trout (Onchorhynchus mykiss) in Iran
title Genetic Characterization of Streptococcus iniae in Diseased Farmed Rainbow Trout (Onchorhynchus mykiss) in Iran
title_full Genetic Characterization of Streptococcus iniae in Diseased Farmed Rainbow Trout (Onchorhynchus mykiss) in Iran
title_fullStr Genetic Characterization of Streptococcus iniae in Diseased Farmed Rainbow Trout (Onchorhynchus mykiss) in Iran
title_full_unstemmed Genetic Characterization of Streptococcus iniae in Diseased Farmed Rainbow Trout (Onchorhynchus mykiss) in Iran
title_short Genetic Characterization of Streptococcus iniae in Diseased Farmed Rainbow Trout (Onchorhynchus mykiss) in Iran
title_sort genetic characterization of streptococcus iniae in diseased farmed rainbow trout (onchorhynchus mykiss) in iran
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3322394/
https://www.ncbi.nlm.nih.gov/pubmed/22550467
http://dx.doi.org/10.1100/2012/594073
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