Immunofluorescence Assay for Serologic Diagnosis of SARS

We evaluated a virus-infected cell-based indirect immunofluorescence assay for detecting anti–severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control wa...

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Autores principales: Chan, Paul K. S., Ng, King-Cheung, Chan, Rickjason C. W., Lam, Rebecca K. Y., Chow, Viola C. Y., Hui, Mamie, Wu, Alan, Lee, Nelson, Yap, Florence H.Y., Cheng, Frankie W. T., Sung, Joseph J. Y., Tam, John S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2004
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3322771/
https://www.ncbi.nlm.nih.gov/pubmed/15109430
http://dx.doi.org/10.3201/eid1003.030493
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author Chan, Paul K. S.
Ng, King-Cheung
Chan, Rickjason C. W.
Lam, Rebecca K. Y.
Chow, Viola C. Y.
Hui, Mamie
Wu, Alan
Lee, Nelson
Yap, Florence H.Y.
Cheng, Frankie W. T.
Sung, Joseph J. Y.
Tam, John S.
author_facet Chan, Paul K. S.
Ng, King-Cheung
Chan, Rickjason C. W.
Lam, Rebecca K. Y.
Chow, Viola C. Y.
Hui, Mamie
Wu, Alan
Lee, Nelson
Yap, Florence H.Y.
Cheng, Frankie W. T.
Sung, Joseph J. Y.
Tam, John S.
author_sort Chan, Paul K. S.
collection PubMed
description We evaluated a virus-infected cell-based indirect immunofluorescence assay for detecting anti–severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection.
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spelling pubmed-33227712012-04-16 Immunofluorescence Assay for Serologic Diagnosis of SARS Chan, Paul K. S. Ng, King-Cheung Chan, Rickjason C. W. Lam, Rebecca K. Y. Chow, Viola C. Y. Hui, Mamie Wu, Alan Lee, Nelson Yap, Florence H.Y. Cheng, Frankie W. T. Sung, Joseph J. Y. Tam, John S. Emerg Infect Dis Dispatch We evaluated a virus-infected cell-based indirect immunofluorescence assay for detecting anti–severe acute respiratory syndrome-associated coronavirus (SARS-CoV) immunoglobulin (Ig) G antibody. All confirmed SARS cases demonstrated seroconversion or fourfold rise in IgG antibody titer; no control was positive. Sensitivity and specificity of this assay were both 100%. Immunofluorescence assay can ascertain the status of SARS-CoV infection. Centers for Disease Control and Prevention 2004-03 /pmc/articles/PMC3322771/ /pubmed/15109430 http://dx.doi.org/10.3201/eid1003.030493 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Chan, Paul K. S.
Ng, King-Cheung
Chan, Rickjason C. W.
Lam, Rebecca K. Y.
Chow, Viola C. Y.
Hui, Mamie
Wu, Alan
Lee, Nelson
Yap, Florence H.Y.
Cheng, Frankie W. T.
Sung, Joseph J. Y.
Tam, John S.
Immunofluorescence Assay for Serologic Diagnosis of SARS
title Immunofluorescence Assay for Serologic Diagnosis of SARS
title_full Immunofluorescence Assay for Serologic Diagnosis of SARS
title_fullStr Immunofluorescence Assay for Serologic Diagnosis of SARS
title_full_unstemmed Immunofluorescence Assay for Serologic Diagnosis of SARS
title_short Immunofluorescence Assay for Serologic Diagnosis of SARS
title_sort immunofluorescence assay for serologic diagnosis of sars
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3322771/
https://www.ncbi.nlm.nih.gov/pubmed/15109430
http://dx.doi.org/10.3201/eid1003.030493
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