Real-Time Reverse Transcription–Polymerase Chain Reaction Assay for SARS-associated Coronavirus

A real-time reverse transcription–polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome–associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discrimina...

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Detalles Bibliográficos
Autores principales: Emery, Shannon L., Erdman, Dean D., Bowen, Michael D., Newton, Bruce R., Winchell, Jonas M., Meyer, Richard F., Tong, Suxiang, Cook, Byron T., Holloway, Brian P., McCaustland, Karen A., Rota, Paul A., Bankamp, Bettina, Lowe, Luis E., Ksiazek, Tom G., Bellini, William J., Anderson, Larry J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2004
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3322901/
https://www.ncbi.nlm.nih.gov/pubmed/15030703
http://dx.doi.org/10.3201/eid1002.030759
Descripción
Sumario:A real-time reverse transcription–polymerase chain reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome–associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARS-CoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RT-PCR assay was more sensitive than a conventional RT-PCR assay or culture isolation and proved suitable to detect SARS-CoV in clinical specimens. Application of this assay will aid in diagnosing SARS-CoV infection.

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