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Serologic and Molecular Biologic Methods for SARS-associated Coronavirus Infection, Taiwan
Severe acute respiratory syndrome (SARS) has raised a global alert since March 2003. After its causative agent, SARS-associated coronavirus (SARS-CoV), was confirmed, laboratory methods, including virus isolation, reverse transcriptase–polymerase chain reaction (RT-PCR), and serologic methods, have...
Autores principales: | , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Centers for Disease Control and Prevention
2004
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3322922/ https://www.ncbi.nlm.nih.gov/pubmed/15030702 http://dx.doi.org/10.3201/eid1002.030731 |
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author | Wu, Ho-Sheng Chiu, Shu-Chun Tseng, Tsan-Chang Lin, Szu-Fong Lin, Jih-Hui Hsu, Yu-Fen Wang, Mei-Ching Lin, Tsuey-Li Yang, Wen-Zieh Ferng, Tian-Lin Huang, Kai-Hung Hsu, Li-Ching Lee, Li-Li Yang, Jyh-Yuan Chen, Hour-Young Su, Shun-Pi Yang, Shih-Yan Lin, Ting-Hsiang Su, Ih-Jen |
author_facet | Wu, Ho-Sheng Chiu, Shu-Chun Tseng, Tsan-Chang Lin, Szu-Fong Lin, Jih-Hui Hsu, Yu-Fen Wang, Mei-Ching Lin, Tsuey-Li Yang, Wen-Zieh Ferng, Tian-Lin Huang, Kai-Hung Hsu, Li-Ching Lee, Li-Li Yang, Jyh-Yuan Chen, Hour-Young Su, Shun-Pi Yang, Shih-Yan Lin, Ting-Hsiang Su, Ih-Jen |
author_sort | Wu, Ho-Sheng |
collection | PubMed |
description | Severe acute respiratory syndrome (SARS) has raised a global alert since March 2003. After its causative agent, SARS-associated coronavirus (SARS-CoV), was confirmed, laboratory methods, including virus isolation, reverse transcriptase–polymerase chain reaction (RT-PCR), and serologic methods, have been quickly developed. In this study, we evaluated four serologic tests ( neutralization test, enzyme-linked immunosorbent assay [ELISA], immunofluorescent assay [IFA], and immunochromatographic test [ICT]) for detecting antibodies to SARS-CoV in sera of 537 probable SARS case-patients with correlation to the RT-PCR . With the neutralization test as a reference method, the sensitivity, specificity, positive predictive value, and negative predictive value were 98.2%, 98.7%, 98.7%, and 98.4% for ELISA; 99.1%, 87.8%, 88.1% and 99.1% for IFA; 33.6%, 98.2%, 95.7%, and 56.1% for ICT, respectively. We also compared the recombinant-based western blot with the whole virus–based IFA and ELISA; the data showed a high correlation between these methods, with an overall agreement of >90%. Our results provide a systematic analysis of serologic and molecular methods for evaluating SARS-CoV infection. |
format | Online Article Text |
id | pubmed-3322922 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2004 |
publisher | Centers for Disease Control and Prevention |
record_format | MEDLINE/PubMed |
spelling | pubmed-33229222012-04-17 Serologic and Molecular Biologic Methods for SARS-associated Coronavirus Infection, Taiwan Wu, Ho-Sheng Chiu, Shu-Chun Tseng, Tsan-Chang Lin, Szu-Fong Lin, Jih-Hui Hsu, Yu-Fen Wang, Mei-Ching Lin, Tsuey-Li Yang, Wen-Zieh Ferng, Tian-Lin Huang, Kai-Hung Hsu, Li-Ching Lee, Li-Li Yang, Jyh-Yuan Chen, Hour-Young Su, Shun-Pi Yang, Shih-Yan Lin, Ting-Hsiang Su, Ih-Jen Emerg Infect Dis Research Severe acute respiratory syndrome (SARS) has raised a global alert since March 2003. After its causative agent, SARS-associated coronavirus (SARS-CoV), was confirmed, laboratory methods, including virus isolation, reverse transcriptase–polymerase chain reaction (RT-PCR), and serologic methods, have been quickly developed. In this study, we evaluated four serologic tests ( neutralization test, enzyme-linked immunosorbent assay [ELISA], immunofluorescent assay [IFA], and immunochromatographic test [ICT]) for detecting antibodies to SARS-CoV in sera of 537 probable SARS case-patients with correlation to the RT-PCR . With the neutralization test as a reference method, the sensitivity, specificity, positive predictive value, and negative predictive value were 98.2%, 98.7%, 98.7%, and 98.4% for ELISA; 99.1%, 87.8%, 88.1% and 99.1% for IFA; 33.6%, 98.2%, 95.7%, and 56.1% for ICT, respectively. We also compared the recombinant-based western blot with the whole virus–based IFA and ELISA; the data showed a high correlation between these methods, with an overall agreement of >90%. Our results provide a systematic analysis of serologic and molecular methods for evaluating SARS-CoV infection. Centers for Disease Control and Prevention 2004-02 /pmc/articles/PMC3322922/ /pubmed/15030702 http://dx.doi.org/10.3201/eid1002.030731 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited. |
spellingShingle | Research Wu, Ho-Sheng Chiu, Shu-Chun Tseng, Tsan-Chang Lin, Szu-Fong Lin, Jih-Hui Hsu, Yu-Fen Wang, Mei-Ching Lin, Tsuey-Li Yang, Wen-Zieh Ferng, Tian-Lin Huang, Kai-Hung Hsu, Li-Ching Lee, Li-Li Yang, Jyh-Yuan Chen, Hour-Young Su, Shun-Pi Yang, Shih-Yan Lin, Ting-Hsiang Su, Ih-Jen Serologic and Molecular Biologic Methods for SARS-associated Coronavirus Infection, Taiwan |
title | Serologic and Molecular Biologic Methods for SARS-associated Coronavirus Infection, Taiwan |
title_full | Serologic and Molecular Biologic Methods for SARS-associated Coronavirus Infection, Taiwan |
title_fullStr | Serologic and Molecular Biologic Methods for SARS-associated Coronavirus Infection, Taiwan |
title_full_unstemmed | Serologic and Molecular Biologic Methods for SARS-associated Coronavirus Infection, Taiwan |
title_short | Serologic and Molecular Biologic Methods for SARS-associated Coronavirus Infection, Taiwan |
title_sort | serologic and molecular biologic methods for sars-associated coronavirus infection, taiwan |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3322922/ https://www.ncbi.nlm.nih.gov/pubmed/15030702 http://dx.doi.org/10.3201/eid1002.030731 |
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