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Serologic and Molecular Biologic Methods for SARS-associated Coronavirus Infection, Taiwan

Severe acute respiratory syndrome (SARS) has raised a global alert since March 2003. After its causative agent, SARS-associated coronavirus (SARS-CoV), was confirmed, laboratory methods, including virus isolation, reverse transcriptase–polymerase chain reaction (RT-PCR), and serologic methods, have...

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Autores principales: Wu, Ho-Sheng, Chiu, Shu-Chun, Tseng, Tsan-Chang, Lin, Szu-Fong, Lin, Jih-Hui, Hsu, Yu-Fen, Wang, Mei-Ching, Lin, Tsuey-Li, Yang, Wen-Zieh, Ferng, Tian-Lin, Huang, Kai-Hung, Hsu, Li-Ching, Lee, Li-Li, Yang, Jyh-Yuan, Chen, Hour-Young, Su, Shun-Pi, Yang, Shih-Yan, Lin, Ting-Hsiang, Su, Ih-Jen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2004
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3322922/
https://www.ncbi.nlm.nih.gov/pubmed/15030702
http://dx.doi.org/10.3201/eid1002.030731
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author Wu, Ho-Sheng
Chiu, Shu-Chun
Tseng, Tsan-Chang
Lin, Szu-Fong
Lin, Jih-Hui
Hsu, Yu-Fen
Wang, Mei-Ching
Lin, Tsuey-Li
Yang, Wen-Zieh
Ferng, Tian-Lin
Huang, Kai-Hung
Hsu, Li-Ching
Lee, Li-Li
Yang, Jyh-Yuan
Chen, Hour-Young
Su, Shun-Pi
Yang, Shih-Yan
Lin, Ting-Hsiang
Su, Ih-Jen
author_facet Wu, Ho-Sheng
Chiu, Shu-Chun
Tseng, Tsan-Chang
Lin, Szu-Fong
Lin, Jih-Hui
Hsu, Yu-Fen
Wang, Mei-Ching
Lin, Tsuey-Li
Yang, Wen-Zieh
Ferng, Tian-Lin
Huang, Kai-Hung
Hsu, Li-Ching
Lee, Li-Li
Yang, Jyh-Yuan
Chen, Hour-Young
Su, Shun-Pi
Yang, Shih-Yan
Lin, Ting-Hsiang
Su, Ih-Jen
author_sort Wu, Ho-Sheng
collection PubMed
description Severe acute respiratory syndrome (SARS) has raised a global alert since March 2003. After its causative agent, SARS-associated coronavirus (SARS-CoV), was confirmed, laboratory methods, including virus isolation, reverse transcriptase–polymerase chain reaction (RT-PCR), and serologic methods, have been quickly developed. In this study, we evaluated four serologic tests ( neutralization test, enzyme-linked immunosorbent assay [ELISA], immunofluorescent assay [IFA], and immunochromatographic test [ICT]) for detecting antibodies to SARS-CoV in sera of 537 probable SARS case-patients with correlation to the RT-PCR . With the neutralization test as a reference method, the sensitivity, specificity, positive predictive value, and negative predictive value were 98.2%, 98.7%, 98.7%, and 98.4% for ELISA; 99.1%, 87.8%, 88.1% and 99.1% for IFA; 33.6%, 98.2%, 95.7%, and 56.1% for ICT, respectively. We also compared the recombinant-based western blot with the whole virus–based IFA and ELISA; the data showed a high correlation between these methods, with an overall agreement of >90%. Our results provide a systematic analysis of serologic and molecular methods for evaluating SARS-CoV infection.
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spelling pubmed-33229222012-04-17 Serologic and Molecular Biologic Methods for SARS-associated Coronavirus Infection, Taiwan Wu, Ho-Sheng Chiu, Shu-Chun Tseng, Tsan-Chang Lin, Szu-Fong Lin, Jih-Hui Hsu, Yu-Fen Wang, Mei-Ching Lin, Tsuey-Li Yang, Wen-Zieh Ferng, Tian-Lin Huang, Kai-Hung Hsu, Li-Ching Lee, Li-Li Yang, Jyh-Yuan Chen, Hour-Young Su, Shun-Pi Yang, Shih-Yan Lin, Ting-Hsiang Su, Ih-Jen Emerg Infect Dis Research Severe acute respiratory syndrome (SARS) has raised a global alert since March 2003. After its causative agent, SARS-associated coronavirus (SARS-CoV), was confirmed, laboratory methods, including virus isolation, reverse transcriptase–polymerase chain reaction (RT-PCR), and serologic methods, have been quickly developed. In this study, we evaluated four serologic tests ( neutralization test, enzyme-linked immunosorbent assay [ELISA], immunofluorescent assay [IFA], and immunochromatographic test [ICT]) for detecting antibodies to SARS-CoV in sera of 537 probable SARS case-patients with correlation to the RT-PCR . With the neutralization test as a reference method, the sensitivity, specificity, positive predictive value, and negative predictive value were 98.2%, 98.7%, 98.7%, and 98.4% for ELISA; 99.1%, 87.8%, 88.1% and 99.1% for IFA; 33.6%, 98.2%, 95.7%, and 56.1% for ICT, respectively. We also compared the recombinant-based western blot with the whole virus–based IFA and ELISA; the data showed a high correlation between these methods, with an overall agreement of >90%. Our results provide a systematic analysis of serologic and molecular methods for evaluating SARS-CoV infection. Centers for Disease Control and Prevention 2004-02 /pmc/articles/PMC3322922/ /pubmed/15030702 http://dx.doi.org/10.3201/eid1002.030731 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Research
Wu, Ho-Sheng
Chiu, Shu-Chun
Tseng, Tsan-Chang
Lin, Szu-Fong
Lin, Jih-Hui
Hsu, Yu-Fen
Wang, Mei-Ching
Lin, Tsuey-Li
Yang, Wen-Zieh
Ferng, Tian-Lin
Huang, Kai-Hung
Hsu, Li-Ching
Lee, Li-Li
Yang, Jyh-Yuan
Chen, Hour-Young
Su, Shun-Pi
Yang, Shih-Yan
Lin, Ting-Hsiang
Su, Ih-Jen
Serologic and Molecular Biologic Methods for SARS-associated Coronavirus Infection, Taiwan
title Serologic and Molecular Biologic Methods for SARS-associated Coronavirus Infection, Taiwan
title_full Serologic and Molecular Biologic Methods for SARS-associated Coronavirus Infection, Taiwan
title_fullStr Serologic and Molecular Biologic Methods for SARS-associated Coronavirus Infection, Taiwan
title_full_unstemmed Serologic and Molecular Biologic Methods for SARS-associated Coronavirus Infection, Taiwan
title_short Serologic and Molecular Biologic Methods for SARS-associated Coronavirus Infection, Taiwan
title_sort serologic and molecular biologic methods for sars-associated coronavirus infection, taiwan
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3322922/
https://www.ncbi.nlm.nih.gov/pubmed/15030702
http://dx.doi.org/10.3201/eid1002.030731
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