Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003

During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3′-end of the viral genome, was established to detect and identify the SARS-associated coronavirus. We describe the use of this assay to s...

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Detalles Bibliográficos
Autores principales: Zhai, Junhui, Briese, Thomas, Dai, Erhei, Wang, Xiaoyi, Pang, Xin, Du, Zongmin, Liu, Haihong, Wang, Jin, Wang, Hongxia, Guo, Zhaobiao, Chen, Zeliang, Jiang, Lingxiao, Zhou, Dongsheng, Han, Yanping, Jabado, Omar, Palacios, Gustavo, Lipkin, W. Ian, Yang, Ruifu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2004
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3322935/
https://www.ncbi.nlm.nih.gov/pubmed/15030701
http://dx.doi.org/10.3201/eid1002.030799
Descripción
Sumario:During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3′-end of the viral genome, was established to detect and identify the SARS-associated coronavirus. We describe the use of this assay to screen >700 clinical samples.

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