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Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003
During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3′-end of the viral genome, was established to detect and identify the SARS-associated coronavirus. We describe the use of this assay to s...
| Autores principales: | , , , , , , , , , , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Centers for Disease Control and Prevention
2004
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3322935/ https://www.ncbi.nlm.nih.gov/pubmed/15030701 http://dx.doi.org/10.3201/eid1002.030799 |
| _version_ | 1782229121343946752 |
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| author | Zhai, Junhui Briese, Thomas Dai, Erhei Wang, Xiaoyi Pang, Xin Du, Zongmin Liu, Haihong Wang, Jin Wang, Hongxia Guo, Zhaobiao Chen, Zeliang Jiang, Lingxiao Zhou, Dongsheng Han, Yanping Jabado, Omar Palacios, Gustavo Lipkin, W. Ian Yang, Ruifu |
| author_facet | Zhai, Junhui Briese, Thomas Dai, Erhei Wang, Xiaoyi Pang, Xin Du, Zongmin Liu, Haihong Wang, Jin Wang, Hongxia Guo, Zhaobiao Chen, Zeliang Jiang, Lingxiao Zhou, Dongsheng Han, Yanping Jabado, Omar Palacios, Gustavo Lipkin, W. Ian Yang, Ruifu |
| author_sort | Zhai, Junhui |
| collection | PubMed |
| description | During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3′-end of the viral genome, was established to detect and identify the SARS-associated coronavirus. We describe the use of this assay to screen >700 clinical samples. |
| format | Online Article Text |
| id | pubmed-3322935 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2004 |
| publisher | Centers for Disease Control and Prevention |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-33229352012-04-17 Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003 Zhai, Junhui Briese, Thomas Dai, Erhei Wang, Xiaoyi Pang, Xin Du, Zongmin Liu, Haihong Wang, Jin Wang, Hongxia Guo, Zhaobiao Chen, Zeliang Jiang, Lingxiao Zhou, Dongsheng Han, Yanping Jabado, Omar Palacios, Gustavo Lipkin, W. Ian Yang, Ruifu Emerg Infect Dis Dispatch During the 2003 severe acute respiratory syndrome (SARS) outbreak, a real-time quantitative polymerase chain reaction, which targets the nucleocapsid gene at the 3′-end of the viral genome, was established to detect and identify the SARS-associated coronavirus. We describe the use of this assay to screen >700 clinical samples. Centers for Disease Control and Prevention 2004-02 /pmc/articles/PMC3322935/ /pubmed/15030701 http://dx.doi.org/10.3201/eid1002.030799 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited. |
| spellingShingle | Dispatch Zhai, Junhui Briese, Thomas Dai, Erhei Wang, Xiaoyi Pang, Xin Du, Zongmin Liu, Haihong Wang, Jin Wang, Hongxia Guo, Zhaobiao Chen, Zeliang Jiang, Lingxiao Zhou, Dongsheng Han, Yanping Jabado, Omar Palacios, Gustavo Lipkin, W. Ian Yang, Ruifu Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003 |
| title | Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003 |
| title_full | Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003 |
| title_fullStr | Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003 |
| title_full_unstemmed | Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003 |
| title_short | Real-Time Polymerase Chain Reaction for Detecting SARS Coronavirus, Beijing, 2003 |
| title_sort | real-time polymerase chain reaction for detecting sars coronavirus, beijing, 2003 |
| topic | Dispatch |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3322935/ https://www.ncbi.nlm.nih.gov/pubmed/15030701 http://dx.doi.org/10.3201/eid1002.030799 |
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