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Assembly, Purification, and Pre-steady-state Kinetic Analysis of Active RNA-dependent RNA Polymerase Elongation Complex

NS5B is the RNA-dependent RNA polymerase responsible for replicating hepatitis C virus (HCV) genomic RNA. Despite more than a decade of work, the formation of a highly active NS5B polymerase·RNA complex suitable for mechanistic and structural studies has remained elusive. Here, we report that throug...

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Detalles Bibliográficos
Autores principales:	Jin, Zhinan, Leveque, Vincent, Ma, Han, Johnson, Kenneth A., Klumpp, Klaus
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Society for Biochemistry and Molecular Biology 2012
Materias:	Enzymology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3323022/ https://www.ncbi.nlm.nih.gov/pubmed/22303022 http://dx.doi.org/10.1074/jbc.M111.325530

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