SARS Coronavirus Detection

We developed a set of three real-time reverse transcription–polymerase chain reaction (PCR) assays that amplify three different regions of the SARS-associated coronavirus (SARS-CoV), can be run in parallel or in a single tube, and can detect <10 genome equivalents of SARS-CoV. The assays consider...

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Detalles Bibliográficos
Autores principales: Nitsche, Andreas, Schweiger, Brunhilde, Ellerbrok, Heinz, Niedrig, Matthias, Pauli, Georg
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2004
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3323308/
https://www.ncbi.nlm.nih.gov/pubmed/15324554
http://dx.doi.org/10.3201/eid1007.030678
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author Nitsche, Andreas
Schweiger, Brunhilde
Ellerbrok, Heinz
Niedrig, Matthias
Pauli, Georg
author_facet Nitsche, Andreas
Schweiger, Brunhilde
Ellerbrok, Heinz
Niedrig, Matthias
Pauli, Georg
author_sort Nitsche, Andreas
collection PubMed
description We developed a set of three real-time reverse transcription–polymerase chain reaction (PCR) assays that amplify three different regions of the SARS-associated coronavirus (SARS-CoV), can be run in parallel or in a single tube, and can detect <10 genome equivalents of SARS-CoV. The assays consider all currently available SARS-CoV sequences and are optimized for two prominent real-time PCR platforms.
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spelling pubmed-33233082012-04-17 SARS Coronavirus Detection Nitsche, Andreas Schweiger, Brunhilde Ellerbrok, Heinz Niedrig, Matthias Pauli, Georg Emerg Infect Dis Dispatch We developed a set of three real-time reverse transcription–polymerase chain reaction (PCR) assays that amplify three different regions of the SARS-associated coronavirus (SARS-CoV), can be run in parallel or in a single tube, and can detect <10 genome equivalents of SARS-CoV. The assays consider all currently available SARS-CoV sequences and are optimized for two prominent real-time PCR platforms. Centers for Disease Control and Prevention 2004-07 /pmc/articles/PMC3323308/ /pubmed/15324554 http://dx.doi.org/10.3201/eid1007.030678 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Nitsche, Andreas
Schweiger, Brunhilde
Ellerbrok, Heinz
Niedrig, Matthias
Pauli, Georg
SARS Coronavirus Detection
title SARS Coronavirus Detection
title_full SARS Coronavirus Detection
title_fullStr SARS Coronavirus Detection
title_full_unstemmed SARS Coronavirus Detection
title_short SARS Coronavirus Detection
title_sort sars coronavirus detection
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3323308/
https://www.ncbi.nlm.nih.gov/pubmed/15324554
http://dx.doi.org/10.3201/eid1007.030678
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