Cargando…

A novel procedure for absolute real-time quantification of gene expression patterns

BACKGROUND: Temporal and tissue-specific patterns of gene expression play important roles in functionality of a biological system. Real-time quantitative polymerase chain reaction (qPCR) technique has been widely applied to single gene expressions, but its potential has not been fully released as mo...

Descripción completa

Detalles Bibliográficos
Autores principales:	Lu, Yingqing, Xie, Lulu, Chen, Jiani
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	BioMed Central 2012
Materias:	Methodology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3323441/ https://www.ncbi.nlm.nih.gov/pubmed/22404915 http://dx.doi.org/10.1186/1746-4811-8-9

Ejemplares similares

Standardization of Gene Expression Quantification by Absolute Real-Time qRT-PCR System Using a Single Standard for Marker and Reference Genes
por: Zhou, Yi-Hong, et al.
Publicado: (2010)

A new protocol for absolute quantification of haemosporidian parasites in raptors and comparison with current assays
por: Huang, Xi, et al.
Publicado: (2020)

A duplex real-time PCR assay for the detection and quantification of avian reovirus and Mycoplasma synoviae
por: Huang, Li, et al.
Publicado: (2015)

A mass spectrometric strategy for absolute quantification of Plasmodium falciparum proteins of low abundance
por: Southworth, Paul M, et al.
Publicado: (2011)

Evaluation of absolute quantitation by nonlinear regression in probe-based real-time PCR
por: Goll, Rasmus, et al.
Publicado: (2006)

Evaluation of a novel real-time PCR assay for the detection, identification and quantification of Plasmodium species causing malaria in humans
por: van Bergen, Kim, et al.
Publicado: (2021)

Absolute estimation of initial concentrations of amplicon in a real-time RT-PCR process
por: Smith, Marjo V, et al.
Publicado: (2007)

Real-Time Vital Mineralization Detection and Quantification during In Vitro Osteoblast Differentiation
por: Serguienko, Anastassia, et al.
Publicado: (2018)

Normalization using ploidy and genomic DNA copy number allows absolute quantification of transcripts, proteins and metabolites in cells
por: Shimada, Hiroshi, et al.
Publicado: (2010)

Absolute quantitative real-time polymerase chain reaction for the measurement of human papillomavirus E7 mRNA in cervical cytobrush specimens
por: Scheurer, Michael E, et al.
Publicado: (2007)

Sensitive and high throughput quantification of abscisic acid based on quantitative real time immuno-PCR
por: Su, Yi, et al.
Publicado: (2018)

Feasibility of absolute quantification for (31)P MRS at 7 T
por: Purvis, Lucian A. B., et al.
Publicado: (2019)

Relative quantification of TCR Vbeta-chain families by real time PCR for identification of clonal T-cell populations
por: Ochsenreither, Sebastian, et al.
Publicado: (2008)

Improving metabolic flux predictions using absolute gene expression data
por: Lee, Dave, et al.
Publicado: (2012)

Selection of reference genes for diurnal and developmental time-course real-time PCR expression analyses in lettuce
por: Sgamma, Tiziana, et al.
Publicado: (2016)

Absolute quantification of the budding yeast transcriptome by means of competitive PCR between genomic and complementary DNAs
por: Miura, Fumihito, et al.
Publicado: (2008)

Real-time PCR quantification of human complement C4A and C4B genes
por: Szilagyi, Agnes, et al.
Publicado: (2006)

Real-time quantification of wild-type contaminants in glyphosate tolerant soybean
por: Battistini, Elena, et al.
Publicado: (2009)

Quantitative Evaluation of CFTR Gene Expression: A Comparison between Relative Quantification by Real-Time PCR and Absolute Quantification by Droplet Digital PCR
por: Bruno, Sabina Maria, et al.
Publicado: (2023)

A Bayesian method for calculating real-time quantitative PCR calibration curves using absolute plasmid DNA standards
por: Sivaganesan, Mano, et al.
Publicado: (2008)

A nested real-time PCR assay for the quantification of Plasmodium falciparum DNA extracted from dried blood spots
por: Tran, Tuan M, et al.
Publicado: (2014)

Development of a DNA-based real-time PCR assay for the quantification of Colletotrichum camelliae growth in tea (Camellia sinensis)
por: He, Shengnan, et al.
Publicado: (2020)

Absolute quantification by droplet digital PCR versus analog real-time PCR
por: Hindson, Christopher M, et al.
Publicado: (2013)

Development of a new ultra sensitive real-time PCR assay (ultra sensitive RTQ-PCR) for the quantification of HBV-DNA
por: Paraskevis, Dimitrios, et al.
Publicado: (2010)

SH2-PLA: a sensitive in-solution approach for quantification of modular domain binding by proximity ligation and real-time PCR
por: Thompson, Christopher M., et al.
Publicado: (2015)

A DNA-based real-time PCR assay for robust growth quantification of the bacterial pathogen Pseudomonas syringae on Arabidopsis thaliana
por: Ross, Annegret, et al.
Publicado: (2016)

Validation of a pan-orthopox real-time PCR assay for the detection and quantification of viral genomes from nonhuman primate blood
por: Mucker, Eric M., et al.
Publicado: (2017)

Identification of reliable reference genes for quantitative real-time PCR normalization in pitaya
por: Chen, Canbin, et al.
Publicado: (2019)

Improving reliability and absolute quantification of human brain microarray data by filtering and scaling probes using RNA-Seq
por: Miller, Jeremy A, et al.
Publicado: (2014)

Absolute quantitation of microbiota abundance in environmental samples
por: Tkacz, Andrzej, et al.
Publicado: (2018)

A novel method to identify gene interaction patterns
por: Lu, Xinguo, et al.
Publicado: (2021)

A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR
por: Rutledge, Robert G, et al.
Publicado: (2008)

Development of a real-time quantitative PCR method for detection and quantification of Prevotella copri
por: Verbrugghe, Phebe, et al.
Publicado: (2021)

Dissecting organ-specific transcriptomes through RNA-sequencing
por: Guan, Shan, et al.
Publicado: (2013)

Critical points of DNA quantification by real-time PCR – effects of DNA extraction method and sample matrix on quantification of genetically modified organisms
por: Cankar, Katarina, et al.
Publicado: (2006)

FCI: an R-based algorithm for evaluating uncertainty of absolute real-time PCR quantification
por: Verderio, Paolo, et al.
Publicado: (2008)

In-flow real-time detection of spectrally encoded microgels for miRNA absolute quantification
por: Dannhauser, David, et al.
Publicado: (2016)

Development and validation of a real-time PCR assay for detection and quantification of Tuber magnatum in soil
por: Iotti, Mirco, et al.
Publicado: (2012)

Comparison of different real-time PCR chemistries and their suitability for detection and quantification of genetically modified organisms
por: Buh Gašparič, Meti, et al.
Publicado: (2008)

Detection and quantification of Flavobacterium psychrophilum in water and fish tissue samples by quantitative real time PCR
por: Strepparava, Nicole, et al.
Publicado: (2014)

Cannot write session to /tmp/vufind_sessions/sess_le5a3dr43disjpevsttvg92k7k