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Role of micronucleus test in predicting breast cancer susceptibility: a systematic review and meta-analysis

BACKGROUND: The cytokinesis-block micronucleus test (MNT), as a marker of chromosomal mutagen sensitivity, was applied in a number of studies enrolling breast cancer (BC) patients and subjects with known or putative genetic predisposition to BC. The large majority of them involve the evaluation of i...

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Autores principales: Cardinale, F, Bruzzi, P, Bolognesi, C
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3324300/
https://www.ncbi.nlm.nih.gov/pubmed/22187037
http://dx.doi.org/10.1038/bjc.2011.567
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author Cardinale, F
Bruzzi, P
Bolognesi, C
author_facet Cardinale, F
Bruzzi, P
Bolognesi, C
author_sort Cardinale, F
collection PubMed
description BACKGROUND: The cytokinesis-block micronucleus test (MNT), as a marker of chromosomal mutagen sensitivity, was applied in a number of studies enrolling breast cancer (BC) patients and subjects with known or putative genetic predisposition to BC. The large majority of them involve the evaluation of induced micronuclei (MN) frequency in peripheral lymphocytes, after the in vitro challenge with ionising radiations. METHODS: The aim of the present systematic review and meta-analysis is to investigate the role of MN assay in the identification of individuals at increased risk of BC and its potential use as prescreening test in women with a family history (FH) of BC. RESULTS: Twelve studies were included in the meta-analysis, covering a time interval 1998–2007, and including 752 cases and 593 controls. Among the cases, 629 are cancer patients and 123 are cancer-free subjects, including 32 first-degree relatives of the susceptible subjects and 91 BRCA1/2 mutation carriers. Our meta-analysis reveals a significant increase of baseline MN frequency related to cancer status, but the association with FH of BC and specifically with BRCA mutations is not clear. A larger difference in MN frequency between cases and controls was observed after in vitro challenge, but response to radiation exposure doesn't appear to better discriminate cancer-susceptible subjects. CONCLUSION: Our study suggests the presence of some bias affecting many of these studies, reinforcing the suggestion that a more rigorous study design is needed in this area.
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spelling pubmed-33243002013-02-14 Role of micronucleus test in predicting breast cancer susceptibility: a systematic review and meta-analysis Cardinale, F Bruzzi, P Bolognesi, C Br J Cancer Genetics and Genomics BACKGROUND: The cytokinesis-block micronucleus test (MNT), as a marker of chromosomal mutagen sensitivity, was applied in a number of studies enrolling breast cancer (BC) patients and subjects with known or putative genetic predisposition to BC. The large majority of them involve the evaluation of induced micronuclei (MN) frequency in peripheral lymphocytes, after the in vitro challenge with ionising radiations. METHODS: The aim of the present systematic review and meta-analysis is to investigate the role of MN assay in the identification of individuals at increased risk of BC and its potential use as prescreening test in women with a family history (FH) of BC. RESULTS: Twelve studies were included in the meta-analysis, covering a time interval 1998–2007, and including 752 cases and 593 controls. Among the cases, 629 are cancer patients and 123 are cancer-free subjects, including 32 first-degree relatives of the susceptible subjects and 91 BRCA1/2 mutation carriers. Our meta-analysis reveals a significant increase of baseline MN frequency related to cancer status, but the association with FH of BC and specifically with BRCA mutations is not clear. A larger difference in MN frequency between cases and controls was observed after in vitro challenge, but response to radiation exposure doesn't appear to better discriminate cancer-susceptible subjects. CONCLUSION: Our study suggests the presence of some bias affecting many of these studies, reinforcing the suggestion that a more rigorous study design is needed in this area. Nature Publishing Group 2012-02-14 2011-12-20 /pmc/articles/PMC3324300/ /pubmed/22187037 http://dx.doi.org/10.1038/bjc.2011.567 Text en Copyright © 2012 Cancer Research UK https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material.If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Genetics and Genomics
Cardinale, F
Bruzzi, P
Bolognesi, C
Role of micronucleus test in predicting breast cancer susceptibility: a systematic review and meta-analysis
title Role of micronucleus test in predicting breast cancer susceptibility: a systematic review and meta-analysis
title_full Role of micronucleus test in predicting breast cancer susceptibility: a systematic review and meta-analysis
title_fullStr Role of micronucleus test in predicting breast cancer susceptibility: a systematic review and meta-analysis
title_full_unstemmed Role of micronucleus test in predicting breast cancer susceptibility: a systematic review and meta-analysis
title_short Role of micronucleus test in predicting breast cancer susceptibility: a systematic review and meta-analysis
title_sort role of micronucleus test in predicting breast cancer susceptibility: a systematic review and meta-analysis
topic Genetics and Genomics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3324300/
https://www.ncbi.nlm.nih.gov/pubmed/22187037
http://dx.doi.org/10.1038/bjc.2011.567
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