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FdeC, a Novel Broadly Conserved Escherichia coli Adhesin Eliciting Protection against Urinary Tract Infections
The increasing antibiotic resistance of pathogenic Escherichia coli species and the absence of a pan-protective vaccine pose major health concerns. We recently identified, by subtractive reverse vaccinology, nine Escherichia coli antigens that protect mice from sepsis. In this study, we characterize...
Autores principales: | , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Microbiology
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3324786/ https://www.ncbi.nlm.nih.gov/pubmed/22496310 http://dx.doi.org/10.1128/mBio.00010-12 |
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author | Nesta, Barbara Spraggon, Glen Alteri, Christopher Gomes Moriel, Danilo Rosini, Roberto Veggi, Daniele Smith, Sara Bertoldi, Isabella Pastorello, Ilaria Ferlenghi, Ilaria Fontana, Maria Rita Frankel, Gad Mobley, Harry L. T. Rappuoli, Rino Pizza, Mariagrazia Serino, Laura Soriani, Marco |
author_facet | Nesta, Barbara Spraggon, Glen Alteri, Christopher Gomes Moriel, Danilo Rosini, Roberto Veggi, Daniele Smith, Sara Bertoldi, Isabella Pastorello, Ilaria Ferlenghi, Ilaria Fontana, Maria Rita Frankel, Gad Mobley, Harry L. T. Rappuoli, Rino Pizza, Mariagrazia Serino, Laura Soriani, Marco |
author_sort | Nesta, Barbara |
collection | PubMed |
description | The increasing antibiotic resistance of pathogenic Escherichia coli species and the absence of a pan-protective vaccine pose major health concerns. We recently identified, by subtractive reverse vaccinology, nine Escherichia coli antigens that protect mice from sepsis. In this study, we characterized one of them, ECOK1_0290, named FdeC (factor adherence E. coli) for its ability to mediate E. coli adhesion to mammalian cells and extracellular matrix. This adhesive propensity was consistent with the X-ray structure of one of the FdeC domains that shows a striking structural homology to Yersinia pseudotuberculosis invasin and enteropathogenic E. coli intimin. Confocal imaging analysis revealed that expression of FdeC on the bacterial surface is triggered by interaction of E. coli with host cells. This phenotype was also observed in bladder tissue sections derived from mice infected with an extraintestinal strain. Indeed, we observed that FdeC contributes to colonization of the bladder and kidney, with the wild-type strain outcompeting the fdeC mutant in cochallenge experiments. Finally, intranasal mucosal immunization with recombinant FdeC significantly reduced kidney colonization in mice challenged transurethrally with uropathogenic E. coli, supporting a role for FdeC in urinary tract infections. |
format | Online Article Text |
id | pubmed-3324786 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | American Society of Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-33247862012-04-16 FdeC, a Novel Broadly Conserved Escherichia coli Adhesin Eliciting Protection against Urinary Tract Infections Nesta, Barbara Spraggon, Glen Alteri, Christopher Gomes Moriel, Danilo Rosini, Roberto Veggi, Daniele Smith, Sara Bertoldi, Isabella Pastorello, Ilaria Ferlenghi, Ilaria Fontana, Maria Rita Frankel, Gad Mobley, Harry L. T. Rappuoli, Rino Pizza, Mariagrazia Serino, Laura Soriani, Marco mBio Research Article The increasing antibiotic resistance of pathogenic Escherichia coli species and the absence of a pan-protective vaccine pose major health concerns. We recently identified, by subtractive reverse vaccinology, nine Escherichia coli antigens that protect mice from sepsis. In this study, we characterized one of them, ECOK1_0290, named FdeC (factor adherence E. coli) for its ability to mediate E. coli adhesion to mammalian cells and extracellular matrix. This adhesive propensity was consistent with the X-ray structure of one of the FdeC domains that shows a striking structural homology to Yersinia pseudotuberculosis invasin and enteropathogenic E. coli intimin. Confocal imaging analysis revealed that expression of FdeC on the bacterial surface is triggered by interaction of E. coli with host cells. This phenotype was also observed in bladder tissue sections derived from mice infected with an extraintestinal strain. Indeed, we observed that FdeC contributes to colonization of the bladder and kidney, with the wild-type strain outcompeting the fdeC mutant in cochallenge experiments. Finally, intranasal mucosal immunization with recombinant FdeC significantly reduced kidney colonization in mice challenged transurethrally with uropathogenic E. coli, supporting a role for FdeC in urinary tract infections. American Society of Microbiology 2012-04-10 /pmc/articles/PMC3324786/ /pubmed/22496310 http://dx.doi.org/10.1128/mBio.00010-12 Text en Copyright © 2012 Nesta et al. http://creativecommons.org/licenses/by-nc-sa/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License (http://creativecommons.org/licenses/by-nc-sa/3.0/) , which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Nesta, Barbara Spraggon, Glen Alteri, Christopher Gomes Moriel, Danilo Rosini, Roberto Veggi, Daniele Smith, Sara Bertoldi, Isabella Pastorello, Ilaria Ferlenghi, Ilaria Fontana, Maria Rita Frankel, Gad Mobley, Harry L. T. Rappuoli, Rino Pizza, Mariagrazia Serino, Laura Soriani, Marco FdeC, a Novel Broadly Conserved Escherichia coli Adhesin Eliciting Protection against Urinary Tract Infections |
title | FdeC, a Novel Broadly Conserved Escherichia coli Adhesin Eliciting Protection against Urinary Tract Infections |
title_full | FdeC, a Novel Broadly Conserved Escherichia coli Adhesin Eliciting Protection against Urinary Tract Infections |
title_fullStr | FdeC, a Novel Broadly Conserved Escherichia coli Adhesin Eliciting Protection against Urinary Tract Infections |
title_full_unstemmed | FdeC, a Novel Broadly Conserved Escherichia coli Adhesin Eliciting Protection against Urinary Tract Infections |
title_short | FdeC, a Novel Broadly Conserved Escherichia coli Adhesin Eliciting Protection against Urinary Tract Infections |
title_sort | fdec, a novel broadly conserved escherichia coli adhesin eliciting protection against urinary tract infections |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3324786/ https://www.ncbi.nlm.nih.gov/pubmed/22496310 http://dx.doi.org/10.1128/mBio.00010-12 |
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