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Drug delivery monitoring by photoacoustic tomography with an ICG encapsulated double emulsion

The absorption spectrum of indocyanine green (ICG), a nontoxic dye used for medical diagnostics, depends upon its concentration as well as the nature of its environment, i.e., the solvent medium into which it is dissolved. In blood, ICG binds with plasma proteins, thus causing changes in its photoac...

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Autores principales: Rajian, Justin Rajesh, Fabiilli, Mario L., Fowlkes, J. Brian, Carson, Paul L., Wang, Xueding
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Optical Society of America 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3324934/
https://www.ncbi.nlm.nih.gov/pubmed/21934797
http://dx.doi.org/10.1364/OE.19.014335
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author Rajian, Justin Rajesh
Fabiilli, Mario L.
Fowlkes, J. Brian
Carson, Paul L.
Wang, Xueding
author_facet Rajian, Justin Rajesh
Fabiilli, Mario L.
Fowlkes, J. Brian
Carson, Paul L.
Wang, Xueding
author_sort Rajian, Justin Rajesh
collection PubMed
description The absorption spectrum of indocyanine green (ICG), a nontoxic dye used for medical diagnostics, depends upon its concentration as well as the nature of its environment, i.e., the solvent medium into which it is dissolved. In blood, ICG binds with plasma proteins, thus causing changes in its photoacoustic spectrum. We successfully encapsulated ICG in an ultrasound-triggerable perfluorocarbon double emulsion that prevents ICG from binding with plasma proteins. Photoacoustic spectral measurements on point target as well as 2-D photoacoustic images of blood vessels revealed that the photoacoustic spectrum changes significantly in blood when the ICG-loaded emulsion undergoes acoustic droplet vaporization (ADV), which is the conversion of liquid droplets into gas bubbles using ultrasound. We propose that these changes in the photoacoustic spectrum of the ICG emulsion in blood, coupled with photoacoustic tomography, could be used to spatially and quantitatively monitor ultrasound initiated drug delivery. In addition, we suggest that the photoacoustic spectral change induced by ultrasound exposure could also be used as contrast in photoacoustic imaging to obtain a background free image.
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spelling pubmed-33249342012-04-12 Drug delivery monitoring by photoacoustic tomography with an ICG encapsulated double emulsion Rajian, Justin Rajesh Fabiilli, Mario L. Fowlkes, J. Brian Carson, Paul L. Wang, Xueding Opt Express Research-Article The absorption spectrum of indocyanine green (ICG), a nontoxic dye used for medical diagnostics, depends upon its concentration as well as the nature of its environment, i.e., the solvent medium into which it is dissolved. In blood, ICG binds with plasma proteins, thus causing changes in its photoacoustic spectrum. We successfully encapsulated ICG in an ultrasound-triggerable perfluorocarbon double emulsion that prevents ICG from binding with plasma proteins. Photoacoustic spectral measurements on point target as well as 2-D photoacoustic images of blood vessels revealed that the photoacoustic spectrum changes significantly in blood when the ICG-loaded emulsion undergoes acoustic droplet vaporization (ADV), which is the conversion of liquid droplets into gas bubbles using ultrasound. We propose that these changes in the photoacoustic spectrum of the ICG emulsion in blood, coupled with photoacoustic tomography, could be used to spatially and quantitatively monitor ultrasound initiated drug delivery. In addition, we suggest that the photoacoustic spectral change induced by ultrasound exposure could also be used as contrast in photoacoustic imaging to obtain a background free image. Optical Society of America 2011-07-12 /pmc/articles/PMC3324934/ /pubmed/21934797 http://dx.doi.org/10.1364/OE.19.014335 Text en ©2011 Optical Society of America http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 Unported License, which permits download and redistribution, provided that the original work is properly cited. This license restricts the article from being modified or used commercially.
spellingShingle Research-Article
Rajian, Justin Rajesh
Fabiilli, Mario L.
Fowlkes, J. Brian
Carson, Paul L.
Wang, Xueding
Drug delivery monitoring by photoacoustic tomography with an ICG encapsulated double emulsion
title Drug delivery monitoring by photoacoustic tomography with an ICG encapsulated double emulsion
title_full Drug delivery monitoring by photoacoustic tomography with an ICG encapsulated double emulsion
title_fullStr Drug delivery monitoring by photoacoustic tomography with an ICG encapsulated double emulsion
title_full_unstemmed Drug delivery monitoring by photoacoustic tomography with an ICG encapsulated double emulsion
title_short Drug delivery monitoring by photoacoustic tomography with an ICG encapsulated double emulsion
title_sort drug delivery monitoring by photoacoustic tomography with an icg encapsulated double emulsion
topic Research-Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3324934/
https://www.ncbi.nlm.nih.gov/pubmed/21934797
http://dx.doi.org/10.1364/OE.19.014335
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