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A New Generation of T7 RNA Polymerase-Independent Inducible Expression Plasmids for Trypanosoma brucei
Expression of transgenes is central to forward and reverse genetic analysis in Trypanosoma brucei. The inducible expression of transgenes in trypanosomes is based on the tetracycline repressor binding to a tetracycline operator to prevent transcription in the absence of tetracycline. The same induci...
Autores principales: | Sunter, Jack, Wickstead, Bill, Gull, Keith, Carrington, Mark |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3325195/ https://www.ncbi.nlm.nih.gov/pubmed/22511983 http://dx.doi.org/10.1371/journal.pone.0035167 |
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