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A New Generation of T7 RNA Polymerase-Independent Inducible Expression Plasmids for Trypanosoma brucei

Expression of transgenes is central to forward and reverse genetic analysis in Trypanosoma brucei. The inducible expression of transgenes in trypanosomes is based on the tetracycline repressor binding to a tetracycline operator to prevent transcription in the absence of tetracycline. The same induci...

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Detalles Bibliográficos
Autores principales: Sunter, Jack, Wickstead, Bill, Gull, Keith, Carrington, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3325195/
https://www.ncbi.nlm.nih.gov/pubmed/22511983
http://dx.doi.org/10.1371/journal.pone.0035167

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