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Junctional Adhesion Molecule 2 Mediates the Interaction between Hatched Blastocyst and Luminal Epithelium: Induction by Progesterone and LIF

BACKGROUND: Junctional adhesion molecule 2 (Jam2) is a member of the JAM superfamily. JAMs are localized at intercellular contacts and participated in the assembly and maintenance of junctions, and control of cell permeability. Because Jam2 is highly expressed in the luminal epithelium on day 4 of p...

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Autores principales: Su, Ren-Wei, Jia, Bo, Ni, Hua, Lei, Wei, Yue, Shun-Li, Feng, Xu-Hui, Deng, Weng-Bo, Liu, Ji-Long, Zhao, Zhen-Ao, Wang, Tong-Song, Yang, Zeng-Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3325240/
https://www.ncbi.nlm.nih.gov/pubmed/22511936
http://dx.doi.org/10.1371/journal.pone.0034325
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author Su, Ren-Wei
Jia, Bo
Ni, Hua
Lei, Wei
Yue, Shun-Li
Feng, Xu-Hui
Deng, Weng-Bo
Liu, Ji-Long
Zhao, Zhen-Ao
Wang, Tong-Song
Yang, Zeng-Ming
author_facet Su, Ren-Wei
Jia, Bo
Ni, Hua
Lei, Wei
Yue, Shun-Li
Feng, Xu-Hui
Deng, Weng-Bo
Liu, Ji-Long
Zhao, Zhen-Ao
Wang, Tong-Song
Yang, Zeng-Ming
author_sort Su, Ren-Wei
collection PubMed
description BACKGROUND: Junctional adhesion molecule 2 (Jam2) is a member of the JAM superfamily. JAMs are localized at intercellular contacts and participated in the assembly and maintenance of junctions, and control of cell permeability. Because Jam2 is highly expressed in the luminal epithelium on day 4 of pregnancy, this study was to determine whether Jam2 plays a role in uterine receptivity and blastocyst attachment in mouse uterus. METHODOLOGY/PRINCIPAL FINDINGS: Jam2 is highly expressed in the uterine luminal epithelium on days 3 and 4 of pregnancy. Progesterone induces Jam2 expression in ovariectomized mice, which is blocked by progesterone antagonist RU486. Jam2 expression on day 4 of pregnancy is also inhibited by RU486 treatment. Leukemia inhibitory factor (LIF) up-regulates Jam2 protein in isolated luminal epithelium from day 4 uterus, which is blocked by S3I-201, a cell-permeable inhibitor for Stat3 phosphorylation. Under adhesion assay, recombinant Jam2 protein increases the rate of blastocyst adhesion. Both soluble recombinant Jam2 and Jam3 can reverse this process. CONCLUSION: Jam2 is highly expressed in the luminal epithelium of receptive uterus and up-regulated by progesterone and LIF via tyrosine phosphorylation of Stat3. Jam2 may play a role in the interaction between hatched blastocyst and receptive uterus.
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spelling pubmed-33252402012-04-17 Junctional Adhesion Molecule 2 Mediates the Interaction between Hatched Blastocyst and Luminal Epithelium: Induction by Progesterone and LIF Su, Ren-Wei Jia, Bo Ni, Hua Lei, Wei Yue, Shun-Li Feng, Xu-Hui Deng, Weng-Bo Liu, Ji-Long Zhao, Zhen-Ao Wang, Tong-Song Yang, Zeng-Ming PLoS One Research Article BACKGROUND: Junctional adhesion molecule 2 (Jam2) is a member of the JAM superfamily. JAMs are localized at intercellular contacts and participated in the assembly and maintenance of junctions, and control of cell permeability. Because Jam2 is highly expressed in the luminal epithelium on day 4 of pregnancy, this study was to determine whether Jam2 plays a role in uterine receptivity and blastocyst attachment in mouse uterus. METHODOLOGY/PRINCIPAL FINDINGS: Jam2 is highly expressed in the uterine luminal epithelium on days 3 and 4 of pregnancy. Progesterone induces Jam2 expression in ovariectomized mice, which is blocked by progesterone antagonist RU486. Jam2 expression on day 4 of pregnancy is also inhibited by RU486 treatment. Leukemia inhibitory factor (LIF) up-regulates Jam2 protein in isolated luminal epithelium from day 4 uterus, which is blocked by S3I-201, a cell-permeable inhibitor for Stat3 phosphorylation. Under adhesion assay, recombinant Jam2 protein increases the rate of blastocyst adhesion. Both soluble recombinant Jam2 and Jam3 can reverse this process. CONCLUSION: Jam2 is highly expressed in the luminal epithelium of receptive uterus and up-regulated by progesterone and LIF via tyrosine phosphorylation of Stat3. Jam2 may play a role in the interaction between hatched blastocyst and receptive uterus. Public Library of Science 2012-04-12 /pmc/articles/PMC3325240/ /pubmed/22511936 http://dx.doi.org/10.1371/journal.pone.0034325 Text en Su et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Su, Ren-Wei
Jia, Bo
Ni, Hua
Lei, Wei
Yue, Shun-Li
Feng, Xu-Hui
Deng, Weng-Bo
Liu, Ji-Long
Zhao, Zhen-Ao
Wang, Tong-Song
Yang, Zeng-Ming
Junctional Adhesion Molecule 2 Mediates the Interaction between Hatched Blastocyst and Luminal Epithelium: Induction by Progesterone and LIF
title Junctional Adhesion Molecule 2 Mediates the Interaction between Hatched Blastocyst and Luminal Epithelium: Induction by Progesterone and LIF
title_full Junctional Adhesion Molecule 2 Mediates the Interaction between Hatched Blastocyst and Luminal Epithelium: Induction by Progesterone and LIF
title_fullStr Junctional Adhesion Molecule 2 Mediates the Interaction between Hatched Blastocyst and Luminal Epithelium: Induction by Progesterone and LIF
title_full_unstemmed Junctional Adhesion Molecule 2 Mediates the Interaction between Hatched Blastocyst and Luminal Epithelium: Induction by Progesterone and LIF
title_short Junctional Adhesion Molecule 2 Mediates the Interaction between Hatched Blastocyst and Luminal Epithelium: Induction by Progesterone and LIF
title_sort junctional adhesion molecule 2 mediates the interaction between hatched blastocyst and luminal epithelium: induction by progesterone and lif
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3325240/
https://www.ncbi.nlm.nih.gov/pubmed/22511936
http://dx.doi.org/10.1371/journal.pone.0034325
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