Cargando…
Heterogeneous Glycation of Cancellous Bone and Its Association with Bone Quality and Fragility
Non-enzymatic glycation (NEG) and enzymatic biochemical processes create crosslinks that modify the extracellular matrix (ECM) and affect the turnover of bone tissue. Because NEG affects turnover and turnover at the local level affects microarchitecture and formation and removal of microdamage, we h...
Autores principales: | , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3325937/ https://www.ncbi.nlm.nih.gov/pubmed/22514706 http://dx.doi.org/10.1371/journal.pone.0035047 |
_version_ | 1782229472498417664 |
---|---|
author | Karim, Lamya Vashishth, Deepak |
author_facet | Karim, Lamya Vashishth, Deepak |
author_sort | Karim, Lamya |
collection | PubMed |
description | Non-enzymatic glycation (NEG) and enzymatic biochemical processes create crosslinks that modify the extracellular matrix (ECM) and affect the turnover of bone tissue. Because NEG affects turnover and turnover at the local level affects microarchitecture and formation and removal of microdamage, we hypothesized that NEG in cancellous bone is heterogeneous and accounts partly for the contribution of microarchitecture and microdamage on bone fragility. Human trabecular bone cores from 23 donors were subjected to compression tests. Mechanically tested cores as well as an additional 19 cores were stained with lead-uranyl acetate and imaged to determine microarchitecture and measure microdamage. Post-yield mechanical properties were measured and damaged trabeculae were extracted from a subset of specimens and characterized for the morphology of induced microdamage. Tested specimens and extracted trabeculae were quantified for enzymatic and non-enzymatic crosslink content using a colorimetric assay and Ultra-high Performance Liquid Chromatography (UPLC). Results show that an increase in enzymatic crosslinks was beneficial for bone where they were associated with increased toughness and decreased microdamage. Conversely, bone with increased NEG required less strain to reach failure and were less tough. NEG heterogeneously modified trabecular microarchitecture where high amounts of NEG crosslinks were found in trabecular rods and with the mechanically deleterious form of microdamage (linear microcracks). The extent of NEG in tibial cancellous bone was the dominant predictor of bone fragility and was associated with changes in microarchitecture and microdamage. |
format | Online Article Text |
id | pubmed-3325937 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-33259372012-04-18 Heterogeneous Glycation of Cancellous Bone and Its Association with Bone Quality and Fragility Karim, Lamya Vashishth, Deepak PLoS One Research Article Non-enzymatic glycation (NEG) and enzymatic biochemical processes create crosslinks that modify the extracellular matrix (ECM) and affect the turnover of bone tissue. Because NEG affects turnover and turnover at the local level affects microarchitecture and formation and removal of microdamage, we hypothesized that NEG in cancellous bone is heterogeneous and accounts partly for the contribution of microarchitecture and microdamage on bone fragility. Human trabecular bone cores from 23 donors were subjected to compression tests. Mechanically tested cores as well as an additional 19 cores were stained with lead-uranyl acetate and imaged to determine microarchitecture and measure microdamage. Post-yield mechanical properties were measured and damaged trabeculae were extracted from a subset of specimens and characterized for the morphology of induced microdamage. Tested specimens and extracted trabeculae were quantified for enzymatic and non-enzymatic crosslink content using a colorimetric assay and Ultra-high Performance Liquid Chromatography (UPLC). Results show that an increase in enzymatic crosslinks was beneficial for bone where they were associated with increased toughness and decreased microdamage. Conversely, bone with increased NEG required less strain to reach failure and were less tough. NEG heterogeneously modified trabecular microarchitecture where high amounts of NEG crosslinks were found in trabecular rods and with the mechanically deleterious form of microdamage (linear microcracks). The extent of NEG in tibial cancellous bone was the dominant predictor of bone fragility and was associated with changes in microarchitecture and microdamage. Public Library of Science 2012-04-13 /pmc/articles/PMC3325937/ /pubmed/22514706 http://dx.doi.org/10.1371/journal.pone.0035047 Text en Karim, Vashishth. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Karim, Lamya Vashishth, Deepak Heterogeneous Glycation of Cancellous Bone and Its Association with Bone Quality and Fragility |
title | Heterogeneous Glycation of Cancellous Bone and Its Association with Bone Quality and Fragility |
title_full | Heterogeneous Glycation of Cancellous Bone and Its Association with Bone Quality and Fragility |
title_fullStr | Heterogeneous Glycation of Cancellous Bone and Its Association with Bone Quality and Fragility |
title_full_unstemmed | Heterogeneous Glycation of Cancellous Bone and Its Association with Bone Quality and Fragility |
title_short | Heterogeneous Glycation of Cancellous Bone and Its Association with Bone Quality and Fragility |
title_sort | heterogeneous glycation of cancellous bone and its association with bone quality and fragility |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3325937/ https://www.ncbi.nlm.nih.gov/pubmed/22514706 http://dx.doi.org/10.1371/journal.pone.0035047 |
work_keys_str_mv | AT karimlamya heterogeneousglycationofcancellousboneanditsassociationwithbonequalityandfragility AT vashishthdeepak heterogeneousglycationofcancellousboneanditsassociationwithbonequalityandfragility |