Micro-Raman Spectroscopy of Silver Nanoparticle Induced Stress on Optically-Trapped Stem Cells

We report here results of a single-cell Raman spectroscopy study of stress effects induced by silver nanoparticles in human mesenchymal stem cells (hMSCs). A high-sensitivity, high-resolution Raman Tweezers set-up has been used to monitor nanoparticle-induced biochemical changes in optically-trapped...

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Autores principales: Bankapur, Aseefhali, Krishnamurthy, R. Sagar, Zachariah, Elsa, Santhosh, Chidangil, Chougule, Basavaraj, Praveen, Bhavishna, Valiathan, Manna, Mathur, Deepak
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3325966/
https://www.ncbi.nlm.nih.gov/pubmed/22514708
http://dx.doi.org/10.1371/journal.pone.0035075
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author Bankapur, Aseefhali
Krishnamurthy, R. Sagar
Zachariah, Elsa
Santhosh, Chidangil
Chougule, Basavaraj
Praveen, Bhavishna
Valiathan, Manna
Mathur, Deepak
author_facet Bankapur, Aseefhali
Krishnamurthy, R. Sagar
Zachariah, Elsa
Santhosh, Chidangil
Chougule, Basavaraj
Praveen, Bhavishna
Valiathan, Manna
Mathur, Deepak
author_sort Bankapur, Aseefhali
collection PubMed
description We report here results of a single-cell Raman spectroscopy study of stress effects induced by silver nanoparticles in human mesenchymal stem cells (hMSCs). A high-sensitivity, high-resolution Raman Tweezers set-up has been used to monitor nanoparticle-induced biochemical changes in optically-trapped single cells. Our micro-Raman spectroscopic study reveals that hMSCs treated with silver nanoparticles undergo oxidative stress at doping levels in excess of 2 µg/ml, with results of a statistical analysis of Raman spectra suggesting that the induced stress becomes more dominant at nanoparticle concentration levels above 3 µg/ml.
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spelling pubmed-33259662012-04-18 Micro-Raman Spectroscopy of Silver Nanoparticle Induced Stress on Optically-Trapped Stem Cells Bankapur, Aseefhali Krishnamurthy, R. Sagar Zachariah, Elsa Santhosh, Chidangil Chougule, Basavaraj Praveen, Bhavishna Valiathan, Manna Mathur, Deepak PLoS One Research Article We report here results of a single-cell Raman spectroscopy study of stress effects induced by silver nanoparticles in human mesenchymal stem cells (hMSCs). A high-sensitivity, high-resolution Raman Tweezers set-up has been used to monitor nanoparticle-induced biochemical changes in optically-trapped single cells. Our micro-Raman spectroscopic study reveals that hMSCs treated with silver nanoparticles undergo oxidative stress at doping levels in excess of 2 µg/ml, with results of a statistical analysis of Raman spectra suggesting that the induced stress becomes more dominant at nanoparticle concentration levels above 3 µg/ml. Public Library of Science 2012-04-13 /pmc/articles/PMC3325966/ /pubmed/22514708 http://dx.doi.org/10.1371/journal.pone.0035075 Text en Bankapur et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Bankapur, Aseefhali
Krishnamurthy, R. Sagar
Zachariah, Elsa
Santhosh, Chidangil
Chougule, Basavaraj
Praveen, Bhavishna
Valiathan, Manna
Mathur, Deepak
Micro-Raman Spectroscopy of Silver Nanoparticle Induced Stress on Optically-Trapped Stem Cells
title Micro-Raman Spectroscopy of Silver Nanoparticle Induced Stress on Optically-Trapped Stem Cells
title_full Micro-Raman Spectroscopy of Silver Nanoparticle Induced Stress on Optically-Trapped Stem Cells
title_fullStr Micro-Raman Spectroscopy of Silver Nanoparticle Induced Stress on Optically-Trapped Stem Cells
title_full_unstemmed Micro-Raman Spectroscopy of Silver Nanoparticle Induced Stress on Optically-Trapped Stem Cells
title_short Micro-Raman Spectroscopy of Silver Nanoparticle Induced Stress on Optically-Trapped Stem Cells
title_sort micro-raman spectroscopy of silver nanoparticle induced stress on optically-trapped stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3325966/
https://www.ncbi.nlm.nih.gov/pubmed/22514708
http://dx.doi.org/10.1371/journal.pone.0035075
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