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Molecular imaging of nuclear factor-Y transcriptional activity maps proliferation sites in live animals

In vivo imaging involving the use of genetically engineered animals is an innovative powerful tool for the noninvasive assessment of the molecular and cellular events that are often targets of therapy. On the basis of the knowledge that the activity of the nuclear factor-Y (NF-Y) transcription facto...

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Autores principales: Goeman, Frauke, Manni, Isabella, Artuso, Simona, Ramachandran, Balaji, Toietta, Gabriele, Bossi, Gianluca, Rando, Gianpaolo, Cencioni, Chiara, Germoni, Sabrina, Straino, Stefania, Capogrossi, Maurizio C., Bacchetti, Silvia, Maggi, Adriana, Sacchi, Ada, Ciana, Paolo, Piaggio, Giulia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3327325/
https://www.ncbi.nlm.nih.gov/pubmed/22379106
http://dx.doi.org/10.1091/mbc.E12-01-0039
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author Goeman, Frauke
Manni, Isabella
Artuso, Simona
Ramachandran, Balaji
Toietta, Gabriele
Bossi, Gianluca
Rando, Gianpaolo
Cencioni, Chiara
Germoni, Sabrina
Straino, Stefania
Capogrossi, Maurizio C.
Bacchetti, Silvia
Maggi, Adriana
Sacchi, Ada
Ciana, Paolo
Piaggio, Giulia
author_facet Goeman, Frauke
Manni, Isabella
Artuso, Simona
Ramachandran, Balaji
Toietta, Gabriele
Bossi, Gianluca
Rando, Gianpaolo
Cencioni, Chiara
Germoni, Sabrina
Straino, Stefania
Capogrossi, Maurizio C.
Bacchetti, Silvia
Maggi, Adriana
Sacchi, Ada
Ciana, Paolo
Piaggio, Giulia
author_sort Goeman, Frauke
collection PubMed
description In vivo imaging involving the use of genetically engineered animals is an innovative powerful tool for the noninvasive assessment of the molecular and cellular events that are often targets of therapy. On the basis of the knowledge that the activity of the nuclear factor-Y (NF-Y) transcription factor is restricted in vitro to proliferating cells, we have generated a transgenic reporter mouse, called MITO-Luc (for mitosis-luciferase), in which an NF-Y–dependent promoter controls luciferase expression. In these mice, bioluminescence imaging of NF-Y activity visualizes areas of physiological cell proliferation and regeneration during response to injury. Using this tool, we highlight for the first time a role of NF-Y activity on hepatocyte proliferation during liver regeneration. MITO-Luc reporter mice should facilitate investigations into the involvement of genes in cell proliferation and provide a useful model for studying aberrant proliferation in disease pathogenesis. They should be also useful in the development of new anti/proproliferative drugs and assessment of their efficacy and side effects on nontarget tissues.
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spelling pubmed-33273252012-06-30 Molecular imaging of nuclear factor-Y transcriptional activity maps proliferation sites in live animals Goeman, Frauke Manni, Isabella Artuso, Simona Ramachandran, Balaji Toietta, Gabriele Bossi, Gianluca Rando, Gianpaolo Cencioni, Chiara Germoni, Sabrina Straino, Stefania Capogrossi, Maurizio C. Bacchetti, Silvia Maggi, Adriana Sacchi, Ada Ciana, Paolo Piaggio, Giulia Mol Biol Cell Articles In vivo imaging involving the use of genetically engineered animals is an innovative powerful tool for the noninvasive assessment of the molecular and cellular events that are often targets of therapy. On the basis of the knowledge that the activity of the nuclear factor-Y (NF-Y) transcription factor is restricted in vitro to proliferating cells, we have generated a transgenic reporter mouse, called MITO-Luc (for mitosis-luciferase), in which an NF-Y–dependent promoter controls luciferase expression. In these mice, bioluminescence imaging of NF-Y activity visualizes areas of physiological cell proliferation and regeneration during response to injury. Using this tool, we highlight for the first time a role of NF-Y activity on hepatocyte proliferation during liver regeneration. MITO-Luc reporter mice should facilitate investigations into the involvement of genes in cell proliferation and provide a useful model for studying aberrant proliferation in disease pathogenesis. They should be also useful in the development of new anti/proproliferative drugs and assessment of their efficacy and side effects on nontarget tissues. The American Society for Cell Biology 2012-04-15 /pmc/articles/PMC3327325/ /pubmed/22379106 http://dx.doi.org/10.1091/mbc.E12-01-0039 Text en © 2012 Goeman et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Goeman, Frauke
Manni, Isabella
Artuso, Simona
Ramachandran, Balaji
Toietta, Gabriele
Bossi, Gianluca
Rando, Gianpaolo
Cencioni, Chiara
Germoni, Sabrina
Straino, Stefania
Capogrossi, Maurizio C.
Bacchetti, Silvia
Maggi, Adriana
Sacchi, Ada
Ciana, Paolo
Piaggio, Giulia
Molecular imaging of nuclear factor-Y transcriptional activity maps proliferation sites in live animals
title Molecular imaging of nuclear factor-Y transcriptional activity maps proliferation sites in live animals
title_full Molecular imaging of nuclear factor-Y transcriptional activity maps proliferation sites in live animals
title_fullStr Molecular imaging of nuclear factor-Y transcriptional activity maps proliferation sites in live animals
title_full_unstemmed Molecular imaging of nuclear factor-Y transcriptional activity maps proliferation sites in live animals
title_short Molecular imaging of nuclear factor-Y transcriptional activity maps proliferation sites in live animals
title_sort molecular imaging of nuclear factor-y transcriptional activity maps proliferation sites in live animals
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3327325/
https://www.ncbi.nlm.nih.gov/pubmed/22379106
http://dx.doi.org/10.1091/mbc.E12-01-0039
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