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A mechanosensory system governs myosin II accumulation in dividing cells

The mitotic spindle is generally considered the initiator of furrow ingression. However, recent studies suggest that furrows can form without spindles, particularly during asymmetric cell division. In Dictyostelium, the mechanoenzyme myosin II and the actin cross-linker cortexillin I form a mechanos...

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Autores principales: Kee, Yee-Seir, Ren, Yixin, Dorfman, Danielle, Iijima, Miho, Firtel, Richard, Iglesias, Pablo A., Robinson, Douglas N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3327329/
https://www.ncbi.nlm.nih.gov/pubmed/22379107
http://dx.doi.org/10.1091/mbc.E11-07-0601
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author Kee, Yee-Seir
Ren, Yixin
Dorfman, Danielle
Iijima, Miho
Firtel, Richard
Iglesias, Pablo A.
Robinson, Douglas N.
author_facet Kee, Yee-Seir
Ren, Yixin
Dorfman, Danielle
Iijima, Miho
Firtel, Richard
Iglesias, Pablo A.
Robinson, Douglas N.
author_sort Kee, Yee-Seir
collection PubMed
description The mitotic spindle is generally considered the initiator of furrow ingression. However, recent studies suggest that furrows can form without spindles, particularly during asymmetric cell division. In Dictyostelium, the mechanoenzyme myosin II and the actin cross-linker cortexillin I form a mechanosensor that responds to mechanical stress, which could account for spindle-independent contractile protein recruitment. Here we show that the regulatory and contractility network composed of myosin II, cortexillin I, IQGAP2, kinesin-6 (kif12), and inner centromeric protein (INCENP) is a mechanical stress–responsive system. Myosin II and cortexillin I form the core mechanosensor, and mechanotransduction is mediated by IQGAP2 to kif12 and INCENP. In addition, IQGAP2 is antagonized by IQGAP1 to modulate the mechanoresponsiveness of the system, suggesting a possible mechanism for discriminating between mechanical and biochemical inputs. Furthermore, IQGAP2 is important for maintaining spindle morphology and kif12 and myosin II cleavage furrow recruitment. Cortexillin II is not directly involved in myosin II mechanosensitive accumulation, but without cortexillin I, cortexillin II's role in membrane–cortex attachment is revealed. Finally, the mitotic spindle is dispensable for the system. Overall, this mechanosensory system is structured like a control system characterized by mechanochemical feedback loops that regulate myosin II localization at sites of mechanical stress and the cleavage furrow.
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spelling pubmed-33273292012-06-30 A mechanosensory system governs myosin II accumulation in dividing cells Kee, Yee-Seir Ren, Yixin Dorfman, Danielle Iijima, Miho Firtel, Richard Iglesias, Pablo A. Robinson, Douglas N. Mol Biol Cell Articles The mitotic spindle is generally considered the initiator of furrow ingression. However, recent studies suggest that furrows can form without spindles, particularly during asymmetric cell division. In Dictyostelium, the mechanoenzyme myosin II and the actin cross-linker cortexillin I form a mechanosensor that responds to mechanical stress, which could account for spindle-independent contractile protein recruitment. Here we show that the regulatory and contractility network composed of myosin II, cortexillin I, IQGAP2, kinesin-6 (kif12), and inner centromeric protein (INCENP) is a mechanical stress–responsive system. Myosin II and cortexillin I form the core mechanosensor, and mechanotransduction is mediated by IQGAP2 to kif12 and INCENP. In addition, IQGAP2 is antagonized by IQGAP1 to modulate the mechanoresponsiveness of the system, suggesting a possible mechanism for discriminating between mechanical and biochemical inputs. Furthermore, IQGAP2 is important for maintaining spindle morphology and kif12 and myosin II cleavage furrow recruitment. Cortexillin II is not directly involved in myosin II mechanosensitive accumulation, but without cortexillin I, cortexillin II's role in membrane–cortex attachment is revealed. Finally, the mitotic spindle is dispensable for the system. Overall, this mechanosensory system is structured like a control system characterized by mechanochemical feedback loops that regulate myosin II localization at sites of mechanical stress and the cleavage furrow. The American Society for Cell Biology 2012-04-15 /pmc/articles/PMC3327329/ /pubmed/22379107 http://dx.doi.org/10.1091/mbc.E11-07-0601 Text en © 2012 Kee et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.
spellingShingle Articles
Kee, Yee-Seir
Ren, Yixin
Dorfman, Danielle
Iijima, Miho
Firtel, Richard
Iglesias, Pablo A.
Robinson, Douglas N.
A mechanosensory system governs myosin II accumulation in dividing cells
title A mechanosensory system governs myosin II accumulation in dividing cells
title_full A mechanosensory system governs myosin II accumulation in dividing cells
title_fullStr A mechanosensory system governs myosin II accumulation in dividing cells
title_full_unstemmed A mechanosensory system governs myosin II accumulation in dividing cells
title_short A mechanosensory system governs myosin II accumulation in dividing cells
title_sort mechanosensory system governs myosin ii accumulation in dividing cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3327329/
https://www.ncbi.nlm.nih.gov/pubmed/22379107
http://dx.doi.org/10.1091/mbc.E11-07-0601
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