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A Cross-Species Analysis of MicroRNAs in the Developing Avian Face

Higher vertebrates use similar genetic tools to derive very different facial features. This diversity is believed to occur through temporal, spatial and species-specific changes in gene expression within cranial neural crest (NC) cells. These contribute to the facial skeleton and contain species-spe...

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Autores principales: Powder, Kara E., Ku, Yuan-Chieh, Brugmann, Samantha A., Veile, Rose A., Renaud, Nicole A., Helms, Jill A., Lovett, Michael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3327661/
https://www.ncbi.nlm.nih.gov/pubmed/22523571
http://dx.doi.org/10.1371/journal.pone.0035111
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author Powder, Kara E.
Ku, Yuan-Chieh
Brugmann, Samantha A.
Veile, Rose A.
Renaud, Nicole A.
Helms, Jill A.
Lovett, Michael
author_facet Powder, Kara E.
Ku, Yuan-Chieh
Brugmann, Samantha A.
Veile, Rose A.
Renaud, Nicole A.
Helms, Jill A.
Lovett, Michael
author_sort Powder, Kara E.
collection PubMed
description Higher vertebrates use similar genetic tools to derive very different facial features. This diversity is believed to occur through temporal, spatial and species-specific changes in gene expression within cranial neural crest (NC) cells. These contribute to the facial skeleton and contain species-specific information that drives morphological variation. A few signaling molecules and transcription factors are known to play important roles in these processes, but little is known regarding the role of micro-RNAs (miRNAs). We have identified and compared all miRNAs expressed in cranial NC cells from three avian species (chicken, duck, and quail) before and after species-specific facial distinctions occur. We identified 170 differentially expressed miRNAs. These include thirty-five novel chicken orthologs of previously described miRNAs, and six avian-specific miRNAs. Five of these avian-specific miRNAs are conserved over 120 million years of avian evolution, from ratites to galliforms, and their predicted target mRNAs include many components of Wnt signaling. Previous work indicates that mRNA gene expression in NC cells is relatively static during stages when the beak acquires species-specific morphologies. However, miRNA expression is remarkably dynamic within this timeframe, suggesting that the timing of specific developmental transitions is altered in birds with different beak shapes. We evaluated one miRNA:mRNA target pair and found that the cell cycle regulator p27(KIP1) is a likely target of miR-222 in frontonasal NC cells, and that the timing of this interaction correlates with the onset of phenotypic variation. Our comparative genomic approach is the first comprehensive analysis of miRNAs in the developing facial primordial, and in species-specific facial development.
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spelling pubmed-33276612012-04-20 A Cross-Species Analysis of MicroRNAs in the Developing Avian Face Powder, Kara E. Ku, Yuan-Chieh Brugmann, Samantha A. Veile, Rose A. Renaud, Nicole A. Helms, Jill A. Lovett, Michael PLoS One Research Article Higher vertebrates use similar genetic tools to derive very different facial features. This diversity is believed to occur through temporal, spatial and species-specific changes in gene expression within cranial neural crest (NC) cells. These contribute to the facial skeleton and contain species-specific information that drives morphological variation. A few signaling molecules and transcription factors are known to play important roles in these processes, but little is known regarding the role of micro-RNAs (miRNAs). We have identified and compared all miRNAs expressed in cranial NC cells from three avian species (chicken, duck, and quail) before and after species-specific facial distinctions occur. We identified 170 differentially expressed miRNAs. These include thirty-five novel chicken orthologs of previously described miRNAs, and six avian-specific miRNAs. Five of these avian-specific miRNAs are conserved over 120 million years of avian evolution, from ratites to galliforms, and their predicted target mRNAs include many components of Wnt signaling. Previous work indicates that mRNA gene expression in NC cells is relatively static during stages when the beak acquires species-specific morphologies. However, miRNA expression is remarkably dynamic within this timeframe, suggesting that the timing of specific developmental transitions is altered in birds with different beak shapes. We evaluated one miRNA:mRNA target pair and found that the cell cycle regulator p27(KIP1) is a likely target of miR-222 in frontonasal NC cells, and that the timing of this interaction correlates with the onset of phenotypic variation. Our comparative genomic approach is the first comprehensive analysis of miRNAs in the developing facial primordial, and in species-specific facial development. Public Library of Science 2012-04-16 /pmc/articles/PMC3327661/ /pubmed/22523571 http://dx.doi.org/10.1371/journal.pone.0035111 Text en Powder et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Powder, Kara E.
Ku, Yuan-Chieh
Brugmann, Samantha A.
Veile, Rose A.
Renaud, Nicole A.
Helms, Jill A.
Lovett, Michael
A Cross-Species Analysis of MicroRNAs in the Developing Avian Face
title A Cross-Species Analysis of MicroRNAs in the Developing Avian Face
title_full A Cross-Species Analysis of MicroRNAs in the Developing Avian Face
title_fullStr A Cross-Species Analysis of MicroRNAs in the Developing Avian Face
title_full_unstemmed A Cross-Species Analysis of MicroRNAs in the Developing Avian Face
title_short A Cross-Species Analysis of MicroRNAs in the Developing Avian Face
title_sort cross-species analysis of micrornas in the developing avian face
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3327661/
https://www.ncbi.nlm.nih.gov/pubmed/22523571
http://dx.doi.org/10.1371/journal.pone.0035111
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