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The Escherichia coli SOS Gene dinF Protects against Oxidative Stress and Bile Salts
DNA is constantly damaged by physical and chemical factors, including reactive oxygen species (ROS), such as superoxide radical (O(2) (−)), hydrogen peroxide (H(2)O(2)) and hydroxyl radical (•OH). Specific mechanisms to protect and repair DNA lesions produced by ROS have been developed in living bei...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3327717/ https://www.ncbi.nlm.nih.gov/pubmed/22523558 http://dx.doi.org/10.1371/journal.pone.0034791 |
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author | Rodríguez-Beltrán, Jerónimo Rodríguez-Rojas, Alexandro Guelfo, Javier R. Couce, Alejandro Blázquez, Jesús |
author_facet | Rodríguez-Beltrán, Jerónimo Rodríguez-Rojas, Alexandro Guelfo, Javier R. Couce, Alejandro Blázquez, Jesús |
author_sort | Rodríguez-Beltrán, Jerónimo |
collection | PubMed |
description | DNA is constantly damaged by physical and chemical factors, including reactive oxygen species (ROS), such as superoxide radical (O(2) (−)), hydrogen peroxide (H(2)O(2)) and hydroxyl radical (•OH). Specific mechanisms to protect and repair DNA lesions produced by ROS have been developed in living beings. In Escherichia coli the SOS system, an inducible response activated to rescue cells from severe DNA damage, is a network that regulates the expression of more than 40 genes in response to this damage, many of them playing important roles in DNA damage tolerance mechanisms. Although the function of most of these genes has been elucidated, the activity of some others, such as dinF, remains unknown. The DinF deduced polypeptide sequence shows a high homology with membrane proteins of the multidrug and toxic compound extrusion (MATE) family. We describe here that expression of dinF protects against bile salts, probably by decreasing the effects of ROS, which is consistent with the observed decrease in H(2)O(2)-killing and protein carbonylation. These results, together with its ability to decrease the level of intracellular ROS, suggests that DinF can detoxify, either direct or indirectly, oxidizing molecules that can damage DNA and proteins from both the bacterial metabolism and the environment. Although the exact mechanism of DinF activity remains to be identified, we describe for the first time a role for dinF. |
format | Online Article Text |
id | pubmed-3327717 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-33277172012-04-20 The Escherichia coli SOS Gene dinF Protects against Oxidative Stress and Bile Salts Rodríguez-Beltrán, Jerónimo Rodríguez-Rojas, Alexandro Guelfo, Javier R. Couce, Alejandro Blázquez, Jesús PLoS One Research Article DNA is constantly damaged by physical and chemical factors, including reactive oxygen species (ROS), such as superoxide radical (O(2) (−)), hydrogen peroxide (H(2)O(2)) and hydroxyl radical (•OH). Specific mechanisms to protect and repair DNA lesions produced by ROS have been developed in living beings. In Escherichia coli the SOS system, an inducible response activated to rescue cells from severe DNA damage, is a network that regulates the expression of more than 40 genes in response to this damage, many of them playing important roles in DNA damage tolerance mechanisms. Although the function of most of these genes has been elucidated, the activity of some others, such as dinF, remains unknown. The DinF deduced polypeptide sequence shows a high homology with membrane proteins of the multidrug and toxic compound extrusion (MATE) family. We describe here that expression of dinF protects against bile salts, probably by decreasing the effects of ROS, which is consistent with the observed decrease in H(2)O(2)-killing and protein carbonylation. These results, together with its ability to decrease the level of intracellular ROS, suggests that DinF can detoxify, either direct or indirectly, oxidizing molecules that can damage DNA and proteins from both the bacterial metabolism and the environment. Although the exact mechanism of DinF activity remains to be identified, we describe for the first time a role for dinF. Public Library of Science 2012-04-16 /pmc/articles/PMC3327717/ /pubmed/22523558 http://dx.doi.org/10.1371/journal.pone.0034791 Text en Rodríguez-Beltrán et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Rodríguez-Beltrán, Jerónimo Rodríguez-Rojas, Alexandro Guelfo, Javier R. Couce, Alejandro Blázquez, Jesús The Escherichia coli SOS Gene dinF Protects against Oxidative Stress and Bile Salts |
title | The Escherichia coli SOS Gene dinF Protects against Oxidative Stress and Bile Salts |
title_full | The Escherichia coli SOS Gene dinF Protects against Oxidative Stress and Bile Salts |
title_fullStr | The Escherichia coli SOS Gene dinF Protects against Oxidative Stress and Bile Salts |
title_full_unstemmed | The Escherichia coli SOS Gene dinF Protects against Oxidative Stress and Bile Salts |
title_short | The Escherichia coli SOS Gene dinF Protects against Oxidative Stress and Bile Salts |
title_sort | escherichia coli sos gene dinf protects against oxidative stress and bile salts |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3327717/ https://www.ncbi.nlm.nih.gov/pubmed/22523558 http://dx.doi.org/10.1371/journal.pone.0034791 |
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