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Interaction of Aspirin (Acetylsalicylic Acid) with Lipid Membranes
We studied the interaction of Aspirin (acetylsalicylic acid) with lipid membranes using x-ray diffraction for bilayers containing up to 50 mol% of aspirin. From 2D x-ray intensity maps that cover large areas of reciprocal space we determined the position of the ASA molecules in the phospholipid bila...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3328472/ https://www.ncbi.nlm.nih.gov/pubmed/22529913 http://dx.doi.org/10.1371/journal.pone.0034357 |
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author | Barrett, Matthew A. Zheng, Songbo Roshankar, Golnaz Alsop, Richard J. Belanger, Randy K. R. Huynh, Chris Kučerka, Norbert Rheinstädter, Maikel C. |
author_facet | Barrett, Matthew A. Zheng, Songbo Roshankar, Golnaz Alsop, Richard J. Belanger, Randy K. R. Huynh, Chris Kučerka, Norbert Rheinstädter, Maikel C. |
author_sort | Barrett, Matthew A. |
collection | PubMed |
description | We studied the interaction of Aspirin (acetylsalicylic acid) with lipid membranes using x-ray diffraction for bilayers containing up to 50 mol% of aspirin. From 2D x-ray intensity maps that cover large areas of reciprocal space we determined the position of the ASA molecules in the phospholipid bilayers and the molecular arrangement of the molecules in the plane of the membranes. We present direct experimental evidence that ASA molecules participate in saturated lipid bilayers of DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine) and preferably reside in the head group region of the membrane. Up to 50 mol% ASA molecules can be dissolved in this type of bilayer before the lateral membrane organization is disturbed and the membranes are found to form an ordered, 2D crystal-like structure. Furthermore, ASA and cholesterol were found to co-exist in saturated lipid bilayers, with the ASA molecules residing in the head group region and the cholesterol molecules participating in the hydrophobic membrane core. |
format | Online Article Text |
id | pubmed-3328472 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-33284722012-04-23 Interaction of Aspirin (Acetylsalicylic Acid) with Lipid Membranes Barrett, Matthew A. Zheng, Songbo Roshankar, Golnaz Alsop, Richard J. Belanger, Randy K. R. Huynh, Chris Kučerka, Norbert Rheinstädter, Maikel C. PLoS One Research Article We studied the interaction of Aspirin (acetylsalicylic acid) with lipid membranes using x-ray diffraction for bilayers containing up to 50 mol% of aspirin. From 2D x-ray intensity maps that cover large areas of reciprocal space we determined the position of the ASA molecules in the phospholipid bilayers and the molecular arrangement of the molecules in the plane of the membranes. We present direct experimental evidence that ASA molecules participate in saturated lipid bilayers of DMPC (1,2-dimyristoyl-sn-glycero-3-phosphocholine) and preferably reside in the head group region of the membrane. Up to 50 mol% ASA molecules can be dissolved in this type of bilayer before the lateral membrane organization is disturbed and the membranes are found to form an ordered, 2D crystal-like structure. Furthermore, ASA and cholesterol were found to co-exist in saturated lipid bilayers, with the ASA molecules residing in the head group region and the cholesterol molecules participating in the hydrophobic membrane core. Public Library of Science 2012-04-17 /pmc/articles/PMC3328472/ /pubmed/22529913 http://dx.doi.org/10.1371/journal.pone.0034357 Text en Barrett et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Barrett, Matthew A. Zheng, Songbo Roshankar, Golnaz Alsop, Richard J. Belanger, Randy K. R. Huynh, Chris Kučerka, Norbert Rheinstädter, Maikel C. Interaction of Aspirin (Acetylsalicylic Acid) with Lipid Membranes |
title | Interaction of Aspirin (Acetylsalicylic Acid) with Lipid Membranes |
title_full | Interaction of Aspirin (Acetylsalicylic Acid) with Lipid Membranes |
title_fullStr | Interaction of Aspirin (Acetylsalicylic Acid) with Lipid Membranes |
title_full_unstemmed | Interaction of Aspirin (Acetylsalicylic Acid) with Lipid Membranes |
title_short | Interaction of Aspirin (Acetylsalicylic Acid) with Lipid Membranes |
title_sort | interaction of aspirin (acetylsalicylic acid) with lipid membranes |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3328472/ https://www.ncbi.nlm.nih.gov/pubmed/22529913 http://dx.doi.org/10.1371/journal.pone.0034357 |
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