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Acatalasemic mice are mildly susceptible to adriamycin nephropathy and exhibit increased albuminuria and glomerulosclerosis

BACKGROUND: Catalase is an important antioxidant enzyme that regulates the level of intracellular hydrogen peroxide and hydroxyl radicals. The effects of catalase deficiency on albuminuria and progressive glomerulosclerosis have not yet been fully elucidated. The adriamycin (ADR) nephropathy model i...

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Autores principales: Takiue, Keiichi, Sugiyama, Hitoshi, Inoue, Tatsuyuki, Morinaga, Hiroshi, Kikumoto, Yoko, Kitagawa, Masashi, Kitamura, Shinji, Maeshima, Yohei, Wang, Da-Hong, Masuoka, Noriyoshi, Ogino, Keiki, Makino, Hirofumi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3329410/
https://www.ncbi.nlm.nih.gov/pubmed/22443450
http://dx.doi.org/10.1186/1471-2369-13-14
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author Takiue, Keiichi
Sugiyama, Hitoshi
Inoue, Tatsuyuki
Morinaga, Hiroshi
Kikumoto, Yoko
Kitagawa, Masashi
Kitamura, Shinji
Maeshima, Yohei
Wang, Da-Hong
Masuoka, Noriyoshi
Ogino, Keiki
Makino, Hirofumi
author_facet Takiue, Keiichi
Sugiyama, Hitoshi
Inoue, Tatsuyuki
Morinaga, Hiroshi
Kikumoto, Yoko
Kitagawa, Masashi
Kitamura, Shinji
Maeshima, Yohei
Wang, Da-Hong
Masuoka, Noriyoshi
Ogino, Keiki
Makino, Hirofumi
author_sort Takiue, Keiichi
collection PubMed
description BACKGROUND: Catalase is an important antioxidant enzyme that regulates the level of intracellular hydrogen peroxide and hydroxyl radicals. The effects of catalase deficiency on albuminuria and progressive glomerulosclerosis have not yet been fully elucidated. The adriamycin (ADR) nephropathy model is considered to be an experimental model of focal segmental glomerulosclerosis. A functional catalase deficiency was hypothesized to exacerbate albuminuria and the progression of glomerulosclerosis in this model. METHODS: ADR was intravenously administered to both homozygous acatalasemic mutant mice (C3H/AnLCs(b)Cs(b)) and control wild-type mice (C3H/AnLCs(a)Cs(a)). The functional and morphological alterations of the kidneys, including albuminuria, renal function, podocytic, glomerular and tubulointerstitial injuries, and the activities of catalase were then compared between the two groups up to 8 weeks after disease induction. Moreover, the presence of a mutation of the toll-like receptor 4 (tlr4) gene, which was previously reported in the C3H/HeJ strain, was investigated in both groups. RESULTS: The ADR-treated mice developed significant albuminuria and glomerulosclerosis, and the degree of these conditions in the ADR-treated acatalasemic mice was higher than that in the wild-type mice. ADR induced progressive renal fibrosis, renal atrophy and lipid peroxide accumulation only in the acatalasemic mice. In addition, the level of catalase activity was significantly lower in the kidneys of the acatalasemic mice than in the wild-type mice during the experimental period. The catalase activity increased after ADR injection in wild-type mice, but the acatalasemic mice did not have the ability to increase their catalase activity under oxidative stress. The C3H/AnL strain was found to be negative for the tlr4 gene mutation. CONCLUSIONS: These data indicate that catalase deficiency plays an important role in the progression of renal injury in the ADR nephropathy model.
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spelling pubmed-33294102012-04-19 Acatalasemic mice are mildly susceptible to adriamycin nephropathy and exhibit increased albuminuria and glomerulosclerosis Takiue, Keiichi Sugiyama, Hitoshi Inoue, Tatsuyuki Morinaga, Hiroshi Kikumoto, Yoko Kitagawa, Masashi Kitamura, Shinji Maeshima, Yohei Wang, Da-Hong Masuoka, Noriyoshi Ogino, Keiki Makino, Hirofumi BMC Nephrol Research Article BACKGROUND: Catalase is an important antioxidant enzyme that regulates the level of intracellular hydrogen peroxide and hydroxyl radicals. The effects of catalase deficiency on albuminuria and progressive glomerulosclerosis have not yet been fully elucidated. The adriamycin (ADR) nephropathy model is considered to be an experimental model of focal segmental glomerulosclerosis. A functional catalase deficiency was hypothesized to exacerbate albuminuria and the progression of glomerulosclerosis in this model. METHODS: ADR was intravenously administered to both homozygous acatalasemic mutant mice (C3H/AnLCs(b)Cs(b)) and control wild-type mice (C3H/AnLCs(a)Cs(a)). The functional and morphological alterations of the kidneys, including albuminuria, renal function, podocytic, glomerular and tubulointerstitial injuries, and the activities of catalase were then compared between the two groups up to 8 weeks after disease induction. Moreover, the presence of a mutation of the toll-like receptor 4 (tlr4) gene, which was previously reported in the C3H/HeJ strain, was investigated in both groups. RESULTS: The ADR-treated mice developed significant albuminuria and glomerulosclerosis, and the degree of these conditions in the ADR-treated acatalasemic mice was higher than that in the wild-type mice. ADR induced progressive renal fibrosis, renal atrophy and lipid peroxide accumulation only in the acatalasemic mice. In addition, the level of catalase activity was significantly lower in the kidneys of the acatalasemic mice than in the wild-type mice during the experimental period. The catalase activity increased after ADR injection in wild-type mice, but the acatalasemic mice did not have the ability to increase their catalase activity under oxidative stress. The C3H/AnL strain was found to be negative for the tlr4 gene mutation. CONCLUSIONS: These data indicate that catalase deficiency plays an important role in the progression of renal injury in the ADR nephropathy model. BioMed Central 2012-03-25 /pmc/articles/PMC3329410/ /pubmed/22443450 http://dx.doi.org/10.1186/1471-2369-13-14 Text en Copyright ©2012 Takiue et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Takiue, Keiichi
Sugiyama, Hitoshi
Inoue, Tatsuyuki
Morinaga, Hiroshi
Kikumoto, Yoko
Kitagawa, Masashi
Kitamura, Shinji
Maeshima, Yohei
Wang, Da-Hong
Masuoka, Noriyoshi
Ogino, Keiki
Makino, Hirofumi
Acatalasemic mice are mildly susceptible to adriamycin nephropathy and exhibit increased albuminuria and glomerulosclerosis
title Acatalasemic mice are mildly susceptible to adriamycin nephropathy and exhibit increased albuminuria and glomerulosclerosis
title_full Acatalasemic mice are mildly susceptible to adriamycin nephropathy and exhibit increased albuminuria and glomerulosclerosis
title_fullStr Acatalasemic mice are mildly susceptible to adriamycin nephropathy and exhibit increased albuminuria and glomerulosclerosis
title_full_unstemmed Acatalasemic mice are mildly susceptible to adriamycin nephropathy and exhibit increased albuminuria and glomerulosclerosis
title_short Acatalasemic mice are mildly susceptible to adriamycin nephropathy and exhibit increased albuminuria and glomerulosclerosis
title_sort acatalasemic mice are mildly susceptible to adriamycin nephropathy and exhibit increased albuminuria and glomerulosclerosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3329410/
https://www.ncbi.nlm.nih.gov/pubmed/22443450
http://dx.doi.org/10.1186/1471-2369-13-14
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