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Post-Weaning Protein Malnutrition Increases Blood Pressure and Induces Endothelial Dysfunctions in Rats

Malnutrition during critical periods in early life may increase the subsequent risk of hypertension and metabolic diseases in adulthood, but the underlying mechanisms are still unclear. We aimed to evaluate the effects of post-weaning protein malnutrition on blood pressure and vascular reactivity in...

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Autores principales: de Belchior, Aucelia C. S., Angeli, Jhuli K., de O. Faria, Thaís, Siman, Fabiana D. M., Silveira, Edna A., Meira, Eduardo F., da Costa, Carlos P., Vassallo, Dalton V., Padilha, Alessandra S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3329540/
https://www.ncbi.nlm.nih.gov/pubmed/22529948
http://dx.doi.org/10.1371/journal.pone.0034876
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author de Belchior, Aucelia C. S.
Angeli, Jhuli K.
de O. Faria, Thaís
Siman, Fabiana D. M.
Silveira, Edna A.
Meira, Eduardo F.
da Costa, Carlos P.
Vassallo, Dalton V.
Padilha, Alessandra S.
author_facet de Belchior, Aucelia C. S.
Angeli, Jhuli K.
de O. Faria, Thaís
Siman, Fabiana D. M.
Silveira, Edna A.
Meira, Eduardo F.
da Costa, Carlos P.
Vassallo, Dalton V.
Padilha, Alessandra S.
author_sort de Belchior, Aucelia C. S.
collection PubMed
description Malnutrition during critical periods in early life may increase the subsequent risk of hypertension and metabolic diseases in adulthood, but the underlying mechanisms are still unclear. We aimed to evaluate the effects of post-weaning protein malnutrition on blood pressure and vascular reactivity in aortic rings (conductance artery) and isolated-perfused tail arteries (resistance artery) from control (fed with Labina®) and post-weaning protein malnutrition rats (offspring that received a diet with low protein content for three months). Systolic and diastolic blood pressure and heart rate increased in the post-weaning protein malnutrition rats. In the aortic rings, reactivity to phenylephrine (10(−10)–3.10(−4) M) was similar in both groups. Endothelium removal or L-NAME (10(−4) M) incubation increased the response to phenylephrine, but the L-NAME effect was greater in the aortic rings from the post-weaning protein malnutrition rats. The protein expression of the endothelial nitric oxide isoform increased in the aortic rings from the post-weaning protein malnutrition rats. Incubation with apocynin (0.3 mM) reduced the response to phenylephrine in both groups, but this effect was higher in the post-weaning protein malnutrition rats, suggesting an increase of superoxide anion release. In the tail artery of the post-weaning protein malnutrition rats, the vascular reactivity to phenylephrine (0.001–300 µg) and the relaxation to acetylcholine (10(−10)–10(−3) M) were increased. Post-weaning protein malnutrition increases blood pressure and induces vascular dysfunction. Although the vascular reactivity in the aortic rings did not change, an increase in superoxide anion and nitric oxide was observed in the post-weaning protein malnutrition rats. However, in the resistance arteries, the increased vascular reactivity may be a potential mechanism underlying the increased blood pressure observed in this model.
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spelling pubmed-33295402012-04-23 Post-Weaning Protein Malnutrition Increases Blood Pressure and Induces Endothelial Dysfunctions in Rats de Belchior, Aucelia C. S. Angeli, Jhuli K. de O. Faria, Thaís Siman, Fabiana D. M. Silveira, Edna A. Meira, Eduardo F. da Costa, Carlos P. Vassallo, Dalton V. Padilha, Alessandra S. PLoS One Research Article Malnutrition during critical periods in early life may increase the subsequent risk of hypertension and metabolic diseases in adulthood, but the underlying mechanisms are still unclear. We aimed to evaluate the effects of post-weaning protein malnutrition on blood pressure and vascular reactivity in aortic rings (conductance artery) and isolated-perfused tail arteries (resistance artery) from control (fed with Labina®) and post-weaning protein malnutrition rats (offspring that received a diet with low protein content for three months). Systolic and diastolic blood pressure and heart rate increased in the post-weaning protein malnutrition rats. In the aortic rings, reactivity to phenylephrine (10(−10)–3.10(−4) M) was similar in both groups. Endothelium removal or L-NAME (10(−4) M) incubation increased the response to phenylephrine, but the L-NAME effect was greater in the aortic rings from the post-weaning protein malnutrition rats. The protein expression of the endothelial nitric oxide isoform increased in the aortic rings from the post-weaning protein malnutrition rats. Incubation with apocynin (0.3 mM) reduced the response to phenylephrine in both groups, but this effect was higher in the post-weaning protein malnutrition rats, suggesting an increase of superoxide anion release. In the tail artery of the post-weaning protein malnutrition rats, the vascular reactivity to phenylephrine (0.001–300 µg) and the relaxation to acetylcholine (10(−10)–10(−3) M) were increased. Post-weaning protein malnutrition increases blood pressure and induces vascular dysfunction. Although the vascular reactivity in the aortic rings did not change, an increase in superoxide anion and nitric oxide was observed in the post-weaning protein malnutrition rats. However, in the resistance arteries, the increased vascular reactivity may be a potential mechanism underlying the increased blood pressure observed in this model. Public Library of Science 2012-04-18 /pmc/articles/PMC3329540/ /pubmed/22529948 http://dx.doi.org/10.1371/journal.pone.0034876 Text en de Belchior et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
de Belchior, Aucelia C. S.
Angeli, Jhuli K.
de O. Faria, Thaís
Siman, Fabiana D. M.
Silveira, Edna A.
Meira, Eduardo F.
da Costa, Carlos P.
Vassallo, Dalton V.
Padilha, Alessandra S.
Post-Weaning Protein Malnutrition Increases Blood Pressure and Induces Endothelial Dysfunctions in Rats
title Post-Weaning Protein Malnutrition Increases Blood Pressure and Induces Endothelial Dysfunctions in Rats
title_full Post-Weaning Protein Malnutrition Increases Blood Pressure and Induces Endothelial Dysfunctions in Rats
title_fullStr Post-Weaning Protein Malnutrition Increases Blood Pressure and Induces Endothelial Dysfunctions in Rats
title_full_unstemmed Post-Weaning Protein Malnutrition Increases Blood Pressure and Induces Endothelial Dysfunctions in Rats
title_short Post-Weaning Protein Malnutrition Increases Blood Pressure and Induces Endothelial Dysfunctions in Rats
title_sort post-weaning protein malnutrition increases blood pressure and induces endothelial dysfunctions in rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3329540/
https://www.ncbi.nlm.nih.gov/pubmed/22529948
http://dx.doi.org/10.1371/journal.pone.0034876
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