Cargando…
The Effect of Storage Temperature and Time on the Concentrations of Bovine Serum Amyloid A and Its Mammary Associated Isoform
The objective of this study was to evaluate the effect of storage under various conditions on the concentrations of major bovine acute phase protein—serum amyloid A, and its mammary isoform. Blood samples were taken from seven clinically healthy calves, and milk samples from six clinically healthy d...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2012
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3329682/ https://www.ncbi.nlm.nih.gov/pubmed/22577611 http://dx.doi.org/10.1155/2012/861458 |
_version_ | 1782229880041111552 |
---|---|
author | Tóthová, Csilla Nagy, Oskar Seidel, Herbert Kováč, Gabriel |
author_facet | Tóthová, Csilla Nagy, Oskar Seidel, Herbert Kováč, Gabriel |
author_sort | Tóthová, Csilla |
collection | PubMed |
description | The objective of this study was to evaluate the effect of storage under various conditions on the concentrations of major bovine acute phase protein—serum amyloid A, and its mammary isoform. Blood samples were taken from seven clinically healthy calves, and milk samples from six clinically healthy dairy cows. The harvested blood serum and the milk samples were fractioned into aliquots. One aliquot was analyzed on the day of collection without storage. The second aliquots were stored at 4°C for 1 day, the remaining aliquots were kept frozen at −18°C for 2, 7, 14, and 21 days, and then analyzed. Blood serum was analyzed for serum amyloid A (SAA). The concentrations of mammary isoform of SAA (M-SAA) were measured in milk samples. Over time, the concentrations of SAA in serum showed a tendency of significant decrease during storage at −18°C (P < 0.01). Similarly, the values of M-SAA decreased significantly in samples maintained at freezer temperatures (P < 0.001). In the refrigerated samples, we found non-significantly lower values of SAA, as well as M-SAA. Presented results indicate that the storage temperature and duration may markedly affect the concentrations of bovine SAA and M-SAA. |
format | Online Article Text |
id | pubmed-3329682 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-33296822012-05-10 The Effect of Storage Temperature and Time on the Concentrations of Bovine Serum Amyloid A and Its Mammary Associated Isoform Tóthová, Csilla Nagy, Oskar Seidel, Herbert Kováč, Gabriel Vet Med Int Research Article The objective of this study was to evaluate the effect of storage under various conditions on the concentrations of major bovine acute phase protein—serum amyloid A, and its mammary isoform. Blood samples were taken from seven clinically healthy calves, and milk samples from six clinically healthy dairy cows. The harvested blood serum and the milk samples were fractioned into aliquots. One aliquot was analyzed on the day of collection without storage. The second aliquots were stored at 4°C for 1 day, the remaining aliquots were kept frozen at −18°C for 2, 7, 14, and 21 days, and then analyzed. Blood serum was analyzed for serum amyloid A (SAA). The concentrations of mammary isoform of SAA (M-SAA) were measured in milk samples. Over time, the concentrations of SAA in serum showed a tendency of significant decrease during storage at −18°C (P < 0.01). Similarly, the values of M-SAA decreased significantly in samples maintained at freezer temperatures (P < 0.001). In the refrigerated samples, we found non-significantly lower values of SAA, as well as M-SAA. Presented results indicate that the storage temperature and duration may markedly affect the concentrations of bovine SAA and M-SAA. Hindawi Publishing Corporation 2012 2012-03-19 /pmc/articles/PMC3329682/ /pubmed/22577611 http://dx.doi.org/10.1155/2012/861458 Text en Copyright © 2012 Csilla Tóthová et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Tóthová, Csilla Nagy, Oskar Seidel, Herbert Kováč, Gabriel The Effect of Storage Temperature and Time on the Concentrations of Bovine Serum Amyloid A and Its Mammary Associated Isoform |
title | The Effect of Storage Temperature and Time on the Concentrations of Bovine Serum Amyloid A and Its Mammary Associated Isoform |
title_full | The Effect of Storage Temperature and Time on the Concentrations of Bovine Serum Amyloid A and Its Mammary Associated Isoform |
title_fullStr | The Effect of Storage Temperature and Time on the Concentrations of Bovine Serum Amyloid A and Its Mammary Associated Isoform |
title_full_unstemmed | The Effect of Storage Temperature and Time on the Concentrations of Bovine Serum Amyloid A and Its Mammary Associated Isoform |
title_short | The Effect of Storage Temperature and Time on the Concentrations of Bovine Serum Amyloid A and Its Mammary Associated Isoform |
title_sort | effect of storage temperature and time on the concentrations of bovine serum amyloid a and its mammary associated isoform |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3329682/ https://www.ncbi.nlm.nih.gov/pubmed/22577611 http://dx.doi.org/10.1155/2012/861458 |
work_keys_str_mv | AT tothovacsilla theeffectofstoragetemperatureandtimeontheconcentrationsofbovineserumamyloidaanditsmammaryassociatedisoform AT nagyoskar theeffectofstoragetemperatureandtimeontheconcentrationsofbovineserumamyloidaanditsmammaryassociatedisoform AT seidelherbert theeffectofstoragetemperatureandtimeontheconcentrationsofbovineserumamyloidaanditsmammaryassociatedisoform AT kovacgabriel theeffectofstoragetemperatureandtimeontheconcentrationsofbovineserumamyloidaanditsmammaryassociatedisoform AT tothovacsilla effectofstoragetemperatureandtimeontheconcentrationsofbovineserumamyloidaanditsmammaryassociatedisoform AT nagyoskar effectofstoragetemperatureandtimeontheconcentrationsofbovineserumamyloidaanditsmammaryassociatedisoform AT seidelherbert effectofstoragetemperatureandtimeontheconcentrationsofbovineserumamyloidaanditsmammaryassociatedisoform AT kovacgabriel effectofstoragetemperatureandtimeontheconcentrationsofbovineserumamyloidaanditsmammaryassociatedisoform |