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The Effect of Storage Temperature and Time on the Concentrations of Bovine Serum Amyloid A and Its Mammary Associated Isoform

The objective of this study was to evaluate the effect of storage under various conditions on the concentrations of major bovine acute phase protein—serum amyloid A, and its mammary isoform. Blood samples were taken from seven clinically healthy calves, and milk samples from six clinically healthy d...

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Detalles Bibliográficos
Autores principales: Tóthová, Csilla, Nagy, Oskar, Seidel, Herbert, Kováč, Gabriel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3329682/
https://www.ncbi.nlm.nih.gov/pubmed/22577611
http://dx.doi.org/10.1155/2012/861458
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author Tóthová, Csilla
Nagy, Oskar
Seidel, Herbert
Kováč, Gabriel
author_facet Tóthová, Csilla
Nagy, Oskar
Seidel, Herbert
Kováč, Gabriel
author_sort Tóthová, Csilla
collection PubMed
description The objective of this study was to evaluate the effect of storage under various conditions on the concentrations of major bovine acute phase protein—serum amyloid A, and its mammary isoform. Blood samples were taken from seven clinically healthy calves, and milk samples from six clinically healthy dairy cows. The harvested blood serum and the milk samples were fractioned into aliquots. One aliquot was analyzed on the day of collection without storage. The second aliquots were stored at 4°C for 1 day, the remaining aliquots were kept frozen at −18°C for 2, 7, 14, and 21 days, and then analyzed. Blood serum was analyzed for serum amyloid A (SAA). The concentrations of mammary isoform of SAA (M-SAA) were measured in milk samples. Over time, the concentrations of SAA in serum showed a tendency of significant decrease during storage at −18°C (P < 0.01). Similarly, the values of M-SAA decreased significantly in samples maintained at freezer temperatures (P < 0.001). In the refrigerated samples, we found non-significantly lower values of SAA, as well as M-SAA. Presented results indicate that the storage temperature and duration may markedly affect the concentrations of bovine SAA and M-SAA.
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spelling pubmed-33296822012-05-10 The Effect of Storage Temperature and Time on the Concentrations of Bovine Serum Amyloid A and Its Mammary Associated Isoform Tóthová, Csilla Nagy, Oskar Seidel, Herbert Kováč, Gabriel Vet Med Int Research Article The objective of this study was to evaluate the effect of storage under various conditions on the concentrations of major bovine acute phase protein—serum amyloid A, and its mammary isoform. Blood samples were taken from seven clinically healthy calves, and milk samples from six clinically healthy dairy cows. The harvested blood serum and the milk samples were fractioned into aliquots. One aliquot was analyzed on the day of collection without storage. The second aliquots were stored at 4°C for 1 day, the remaining aliquots were kept frozen at −18°C for 2, 7, 14, and 21 days, and then analyzed. Blood serum was analyzed for serum amyloid A (SAA). The concentrations of mammary isoform of SAA (M-SAA) were measured in milk samples. Over time, the concentrations of SAA in serum showed a tendency of significant decrease during storage at −18°C (P < 0.01). Similarly, the values of M-SAA decreased significantly in samples maintained at freezer temperatures (P < 0.001). In the refrigerated samples, we found non-significantly lower values of SAA, as well as M-SAA. Presented results indicate that the storage temperature and duration may markedly affect the concentrations of bovine SAA and M-SAA. Hindawi Publishing Corporation 2012 2012-03-19 /pmc/articles/PMC3329682/ /pubmed/22577611 http://dx.doi.org/10.1155/2012/861458 Text en Copyright © 2012 Csilla Tóthová et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tóthová, Csilla
Nagy, Oskar
Seidel, Herbert
Kováč, Gabriel
The Effect of Storage Temperature and Time on the Concentrations of Bovine Serum Amyloid A and Its Mammary Associated Isoform
title The Effect of Storage Temperature and Time on the Concentrations of Bovine Serum Amyloid A and Its Mammary Associated Isoform
title_full The Effect of Storage Temperature and Time on the Concentrations of Bovine Serum Amyloid A and Its Mammary Associated Isoform
title_fullStr The Effect of Storage Temperature and Time on the Concentrations of Bovine Serum Amyloid A and Its Mammary Associated Isoform
title_full_unstemmed The Effect of Storage Temperature and Time on the Concentrations of Bovine Serum Amyloid A and Its Mammary Associated Isoform
title_short The Effect of Storage Temperature and Time on the Concentrations of Bovine Serum Amyloid A and Its Mammary Associated Isoform
title_sort effect of storage temperature and time on the concentrations of bovine serum amyloid a and its mammary associated isoform
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3329682/
https://www.ncbi.nlm.nih.gov/pubmed/22577611
http://dx.doi.org/10.1155/2012/861458
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