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Impact of gamma rays on the Phaffia rhodozyma genome revealed by RAPD-PCR

BACKGROUND AND OBJECTIVES: Phaffia rhodozyma is a red yeast which produces astaxanthin as the major carotenoid pigment. Astaxanthin is thought to reduce the incidence of cancer and degenerative diseases in man. It also enhances the immune response and acts as a free-radical quencher, a precursor of...

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Autores principales: Najafi, N, Hosseini, Ramin, Ahmadi, AR
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3330186/
https://www.ncbi.nlm.nih.gov/pubmed/22530091
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author Najafi, N
Hosseini, Ramin
Ahmadi, AR
author_facet Najafi, N
Hosseini, Ramin
Ahmadi, AR
author_sort Najafi, N
collection PubMed
description BACKGROUND AND OBJECTIVES: Phaffia rhodozyma is a red yeast which produces astaxanthin as the major carotenoid pigment. Astaxanthin is thought to reduce the incidence of cancer and degenerative diseases in man. It also enhances the immune response and acts as a free-radical quencher, a precursor of vitamin A, or a pigment involved in the visual attraction of animals as mating partners. The impact of gamma irradiation was studied on the Phaffia rhodozyma genome. MATERIALS AND METHODS: Ten mutant strains, designated Gam1-Gam10, were obtained using gamma irradiation. Ten decamer random amplified polymorphic DNA (RAPD) primers were employed to assess genetic changes. RESULTS: Nine primers revealed scorable polymorphisms and a total of 95 band positions were scored; amongst which 38 bands (37.5%) were polymorphic. Primer F with 3 bands and primer J20 with 13 bands produced the lowest and the highest number of bands, respectively. Primer A16 produced the highest number of polymorphic bands (70% polymorphism) and primer F showed the lowest number of polymorphic bands (0% polymorphism). Genetic distances were calculated using Jaccard's coefficient and the UPGMA method. A dendrogram was created using SPSS (version 11.5) and the strains were clustered into four groups. CONCLUSION: RAPD markers could distinguish between the parental and the mutant strains of P. rhodozyma. RAPD technique showed that some changes had occurred in the genome of the mutated strains. This technique demonstrated the capability to differentiate between the parental and the mutant strains.
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spelling pubmed-33301862012-04-23 Impact of gamma rays on the Phaffia rhodozyma genome revealed by RAPD-PCR Najafi, N Hosseini, Ramin Ahmadi, AR Iran J Microbiol Original Article BACKGROUND AND OBJECTIVES: Phaffia rhodozyma is a red yeast which produces astaxanthin as the major carotenoid pigment. Astaxanthin is thought to reduce the incidence of cancer and degenerative diseases in man. It also enhances the immune response and acts as a free-radical quencher, a precursor of vitamin A, or a pigment involved in the visual attraction of animals as mating partners. The impact of gamma irradiation was studied on the Phaffia rhodozyma genome. MATERIALS AND METHODS: Ten mutant strains, designated Gam1-Gam10, were obtained using gamma irradiation. Ten decamer random amplified polymorphic DNA (RAPD) primers were employed to assess genetic changes. RESULTS: Nine primers revealed scorable polymorphisms and a total of 95 band positions were scored; amongst which 38 bands (37.5%) were polymorphic. Primer F with 3 bands and primer J20 with 13 bands produced the lowest and the highest number of bands, respectively. Primer A16 produced the highest number of polymorphic bands (70% polymorphism) and primer F showed the lowest number of polymorphic bands (0% polymorphism). Genetic distances were calculated using Jaccard's coefficient and the UPGMA method. A dendrogram was created using SPSS (version 11.5) and the strains were clustered into four groups. CONCLUSION: RAPD markers could distinguish between the parental and the mutant strains of P. rhodozyma. RAPD technique showed that some changes had occurred in the genome of the mutated strains. This technique demonstrated the capability to differentiate between the parental and the mutant strains. Tehran University of Medical Sciences 2011-12 /pmc/articles/PMC3330186/ /pubmed/22530091 Text en © 2011 Iranian Society of Microbiology & Tehran University of Medical Sciences http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Najafi, N
Hosseini, Ramin
Ahmadi, AR
Impact of gamma rays on the Phaffia rhodozyma genome revealed by RAPD-PCR
title Impact of gamma rays on the Phaffia rhodozyma genome revealed by RAPD-PCR
title_full Impact of gamma rays on the Phaffia rhodozyma genome revealed by RAPD-PCR
title_fullStr Impact of gamma rays on the Phaffia rhodozyma genome revealed by RAPD-PCR
title_full_unstemmed Impact of gamma rays on the Phaffia rhodozyma genome revealed by RAPD-PCR
title_short Impact of gamma rays on the Phaffia rhodozyma genome revealed by RAPD-PCR
title_sort impact of gamma rays on the phaffia rhodozyma genome revealed by rapd-pcr
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3330186/
https://www.ncbi.nlm.nih.gov/pubmed/22530091
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