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Identification and Characterization of Novel Genotoxic Stress-Inducible Nuclear Long Noncoding RNAs in Mammalian Cells
Whole transcriptome analyses have revealed a large number of novel transcripts including long and short noncoding RNAs (ncRNAs). Currently, there is great interest in characterizing the functions of the different classes of ncRNAs and their relevance to cellular processes. In particular, nuclear lon...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3330809/ https://www.ncbi.nlm.nih.gov/pubmed/22532836 http://dx.doi.org/10.1371/journal.pone.0034949 |
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author | Mizutani, Rena Wakamatsu, Ai Tanaka, Noriyuki Yoshida, Hiroshi Tochigi, Naobumi Suzuki, Yoshio Oonishi, Tadahiro Tani, Hidenori Tano, Keiko Ijiri, Kenichi Isogai, Takao Akimitsu, Nobuyoshi |
author_facet | Mizutani, Rena Wakamatsu, Ai Tanaka, Noriyuki Yoshida, Hiroshi Tochigi, Naobumi Suzuki, Yoshio Oonishi, Tadahiro Tani, Hidenori Tano, Keiko Ijiri, Kenichi Isogai, Takao Akimitsu, Nobuyoshi |
author_sort | Mizutani, Rena |
collection | PubMed |
description | Whole transcriptome analyses have revealed a large number of novel transcripts including long and short noncoding RNAs (ncRNAs). Currently, there is great interest in characterizing the functions of the different classes of ncRNAs and their relevance to cellular processes. In particular, nuclear long ncRNAs may be involved in controlling various aspects of biological regulation, such as stress responses. By a combination of bioinformatic and experimental approaches, we identified 25 novel nuclear long ncRNAs from 6,088,565 full-length human cDNA sequences. Some nuclear long ncRNAs were conserved among vertebrates, whereas others were found only among primates. Expression profiling of the nuclear long ncRNAs in human tissues revealed that most were expressed ubiquitously. A subset of the identified nuclear long ncRNAs was induced by the genotoxic agents mitomycin C or doxorubicin, in HeLa Tet-off cells. There were no commonly altered nuclear long ncRNAs between mitomycin C- and doxorubicin-treated cells. These results suggest that distinct sets of nuclear long ncRNAs play roles in cellular defense mechanisms against specific genotoxic agents, and that particular long ncRNAs have the potential to be surrogate indicators of a specific cell stress. |
format | Online Article Text |
id | pubmed-3330809 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-33308092012-04-24 Identification and Characterization of Novel Genotoxic Stress-Inducible Nuclear Long Noncoding RNAs in Mammalian Cells Mizutani, Rena Wakamatsu, Ai Tanaka, Noriyuki Yoshida, Hiroshi Tochigi, Naobumi Suzuki, Yoshio Oonishi, Tadahiro Tani, Hidenori Tano, Keiko Ijiri, Kenichi Isogai, Takao Akimitsu, Nobuyoshi PLoS One Research Article Whole transcriptome analyses have revealed a large number of novel transcripts including long and short noncoding RNAs (ncRNAs). Currently, there is great interest in characterizing the functions of the different classes of ncRNAs and their relevance to cellular processes. In particular, nuclear long ncRNAs may be involved in controlling various aspects of biological regulation, such as stress responses. By a combination of bioinformatic and experimental approaches, we identified 25 novel nuclear long ncRNAs from 6,088,565 full-length human cDNA sequences. Some nuclear long ncRNAs were conserved among vertebrates, whereas others were found only among primates. Expression profiling of the nuclear long ncRNAs in human tissues revealed that most were expressed ubiquitously. A subset of the identified nuclear long ncRNAs was induced by the genotoxic agents mitomycin C or doxorubicin, in HeLa Tet-off cells. There were no commonly altered nuclear long ncRNAs between mitomycin C- and doxorubicin-treated cells. These results suggest that distinct sets of nuclear long ncRNAs play roles in cellular defense mechanisms against specific genotoxic agents, and that particular long ncRNAs have the potential to be surrogate indicators of a specific cell stress. Public Library of Science 2012-04-19 /pmc/articles/PMC3330809/ /pubmed/22532836 http://dx.doi.org/10.1371/journal.pone.0034949 Text en Mizutani et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Mizutani, Rena Wakamatsu, Ai Tanaka, Noriyuki Yoshida, Hiroshi Tochigi, Naobumi Suzuki, Yoshio Oonishi, Tadahiro Tani, Hidenori Tano, Keiko Ijiri, Kenichi Isogai, Takao Akimitsu, Nobuyoshi Identification and Characterization of Novel Genotoxic Stress-Inducible Nuclear Long Noncoding RNAs in Mammalian Cells |
title | Identification and Characterization of Novel Genotoxic Stress-Inducible Nuclear Long Noncoding RNAs in Mammalian Cells |
title_full | Identification and Characterization of Novel Genotoxic Stress-Inducible Nuclear Long Noncoding RNAs in Mammalian Cells |
title_fullStr | Identification and Characterization of Novel Genotoxic Stress-Inducible Nuclear Long Noncoding RNAs in Mammalian Cells |
title_full_unstemmed | Identification and Characterization of Novel Genotoxic Stress-Inducible Nuclear Long Noncoding RNAs in Mammalian Cells |
title_short | Identification and Characterization of Novel Genotoxic Stress-Inducible Nuclear Long Noncoding RNAs in Mammalian Cells |
title_sort | identification and characterization of novel genotoxic stress-inducible nuclear long noncoding rnas in mammalian cells |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3330809/ https://www.ncbi.nlm.nih.gov/pubmed/22532836 http://dx.doi.org/10.1371/journal.pone.0034949 |
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