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Identification and Characterization of Novel Genotoxic Stress-Inducible Nuclear Long Noncoding RNAs in Mammalian Cells

Whole transcriptome analyses have revealed a large number of novel transcripts including long and short noncoding RNAs (ncRNAs). Currently, there is great interest in characterizing the functions of the different classes of ncRNAs and their relevance to cellular processes. In particular, nuclear lon...

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Autores principales: Mizutani, Rena, Wakamatsu, Ai, Tanaka, Noriyuki, Yoshida, Hiroshi, Tochigi, Naobumi, Suzuki, Yoshio, Oonishi, Tadahiro, Tani, Hidenori, Tano, Keiko, Ijiri, Kenichi, Isogai, Takao, Akimitsu, Nobuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3330809/
https://www.ncbi.nlm.nih.gov/pubmed/22532836
http://dx.doi.org/10.1371/journal.pone.0034949
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author Mizutani, Rena
Wakamatsu, Ai
Tanaka, Noriyuki
Yoshida, Hiroshi
Tochigi, Naobumi
Suzuki, Yoshio
Oonishi, Tadahiro
Tani, Hidenori
Tano, Keiko
Ijiri, Kenichi
Isogai, Takao
Akimitsu, Nobuyoshi
author_facet Mizutani, Rena
Wakamatsu, Ai
Tanaka, Noriyuki
Yoshida, Hiroshi
Tochigi, Naobumi
Suzuki, Yoshio
Oonishi, Tadahiro
Tani, Hidenori
Tano, Keiko
Ijiri, Kenichi
Isogai, Takao
Akimitsu, Nobuyoshi
author_sort Mizutani, Rena
collection PubMed
description Whole transcriptome analyses have revealed a large number of novel transcripts including long and short noncoding RNAs (ncRNAs). Currently, there is great interest in characterizing the functions of the different classes of ncRNAs and their relevance to cellular processes. In particular, nuclear long ncRNAs may be involved in controlling various aspects of biological regulation, such as stress responses. By a combination of bioinformatic and experimental approaches, we identified 25 novel nuclear long ncRNAs from 6,088,565 full-length human cDNA sequences. Some nuclear long ncRNAs were conserved among vertebrates, whereas others were found only among primates. Expression profiling of the nuclear long ncRNAs in human tissues revealed that most were expressed ubiquitously. A subset of the identified nuclear long ncRNAs was induced by the genotoxic agents mitomycin C or doxorubicin, in HeLa Tet-off cells. There were no commonly altered nuclear long ncRNAs between mitomycin C- and doxorubicin-treated cells. These results suggest that distinct sets of nuclear long ncRNAs play roles in cellular defense mechanisms against specific genotoxic agents, and that particular long ncRNAs have the potential to be surrogate indicators of a specific cell stress.
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spelling pubmed-33308092012-04-24 Identification and Characterization of Novel Genotoxic Stress-Inducible Nuclear Long Noncoding RNAs in Mammalian Cells Mizutani, Rena Wakamatsu, Ai Tanaka, Noriyuki Yoshida, Hiroshi Tochigi, Naobumi Suzuki, Yoshio Oonishi, Tadahiro Tani, Hidenori Tano, Keiko Ijiri, Kenichi Isogai, Takao Akimitsu, Nobuyoshi PLoS One Research Article Whole transcriptome analyses have revealed a large number of novel transcripts including long and short noncoding RNAs (ncRNAs). Currently, there is great interest in characterizing the functions of the different classes of ncRNAs and their relevance to cellular processes. In particular, nuclear long ncRNAs may be involved in controlling various aspects of biological regulation, such as stress responses. By a combination of bioinformatic and experimental approaches, we identified 25 novel nuclear long ncRNAs from 6,088,565 full-length human cDNA sequences. Some nuclear long ncRNAs were conserved among vertebrates, whereas others were found only among primates. Expression profiling of the nuclear long ncRNAs in human tissues revealed that most were expressed ubiquitously. A subset of the identified nuclear long ncRNAs was induced by the genotoxic agents mitomycin C or doxorubicin, in HeLa Tet-off cells. There were no commonly altered nuclear long ncRNAs between mitomycin C- and doxorubicin-treated cells. These results suggest that distinct sets of nuclear long ncRNAs play roles in cellular defense mechanisms against specific genotoxic agents, and that particular long ncRNAs have the potential to be surrogate indicators of a specific cell stress. Public Library of Science 2012-04-19 /pmc/articles/PMC3330809/ /pubmed/22532836 http://dx.doi.org/10.1371/journal.pone.0034949 Text en Mizutani et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Mizutani, Rena
Wakamatsu, Ai
Tanaka, Noriyuki
Yoshida, Hiroshi
Tochigi, Naobumi
Suzuki, Yoshio
Oonishi, Tadahiro
Tani, Hidenori
Tano, Keiko
Ijiri, Kenichi
Isogai, Takao
Akimitsu, Nobuyoshi
Identification and Characterization of Novel Genotoxic Stress-Inducible Nuclear Long Noncoding RNAs in Mammalian Cells
title Identification and Characterization of Novel Genotoxic Stress-Inducible Nuclear Long Noncoding RNAs in Mammalian Cells
title_full Identification and Characterization of Novel Genotoxic Stress-Inducible Nuclear Long Noncoding RNAs in Mammalian Cells
title_fullStr Identification and Characterization of Novel Genotoxic Stress-Inducible Nuclear Long Noncoding RNAs in Mammalian Cells
title_full_unstemmed Identification and Characterization of Novel Genotoxic Stress-Inducible Nuclear Long Noncoding RNAs in Mammalian Cells
title_short Identification and Characterization of Novel Genotoxic Stress-Inducible Nuclear Long Noncoding RNAs in Mammalian Cells
title_sort identification and characterization of novel genotoxic stress-inducible nuclear long noncoding rnas in mammalian cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3330809/
https://www.ncbi.nlm.nih.gov/pubmed/22532836
http://dx.doi.org/10.1371/journal.pone.0034949
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