Conservation of an endemic medicinal plant, Berberis tinctoria Lesch. In Nilgiris through micro propagation

Berberis tinctoria Lesch. Is an endemic plant to high hills of Nilgiris having lot of medicinal properties. For its better conservation through mass multiplication, attempts have been made to standardize tissue culture technology. The results of the study exhibited that the basal medium containing BAP and NAA each at 0.5 mg/1 was found to be the optimum for callus formation. Shoot proliferation was highly effective in the basal medium supplemented with BAP at 0.5 mg/1. The root initiation was maximum in the basal medium containing the NAA at 1.0 mg/1 and the plantlet establishment was successful in the hardening medium composed by vermiculite and soil in the ration of 1:1.