DETERMINATION OF PUERARIN FROM PUERARIA TUBEROSA DC BY HPLC

A reverse phase High pressure liquid chromatography (HPLC) method to determine puerarin in Pueraria tuberose was developed and described. The method involves separation of active compound using mobile phase consisting of acetonitrile: water: orthophosporic acid in the ratio of 32:67:01 and detecting...

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Detalles Bibliográficos
Autores principales: Chauhan, Sateesh Kumar, Singh, Brijpal, Agrawal, Sudhakar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 2004
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3330973/
https://www.ncbi.nlm.nih.gov/pubmed/22557128
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author Chauhan, Sateesh Kumar
Singh, Brijpal
Agrawal, Sudhakar
author_facet Chauhan, Sateesh Kumar
Singh, Brijpal
Agrawal, Sudhakar
author_sort Chauhan, Sateesh Kumar
collection PubMed
description A reverse phase High pressure liquid chromatography (HPLC) method to determine puerarin in Pueraria tuberose was developed and described. The method involves separation of active compound using mobile phase consisting of acetonitrile: water: orthophosporic acid in the ratio of 32:67:01 and detecting the chromatogram at 280 nm using photodiode array detector. The sensitivity of the method was observed as 1.0 mg and linearity was observed between 1.0 mg to 8.0mg. The puerarin content of 3.02% w/w was found in crude P.tuberosa The proposed method in precise, sensitive, reproducible and easy to perform which can be used for detection, monitoring and quantification of puerarin in P . tuberose.
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spelling pubmed-33309732012-04-27 DETERMINATION OF PUERARIN FROM PUERARIA TUBEROSA DC BY HPLC Chauhan, Sateesh Kumar Singh, Brijpal Agrawal, Sudhakar Anc Sci Life Original Article A reverse phase High pressure liquid chromatography (HPLC) method to determine puerarin in Pueraria tuberose was developed and described. The method involves separation of active compound using mobile phase consisting of acetonitrile: water: orthophosporic acid in the ratio of 32:67:01 and detecting the chromatogram at 280 nm using photodiode array detector. The sensitivity of the method was observed as 1.0 mg and linearity was observed between 1.0 mg to 8.0mg. The puerarin content of 3.02% w/w was found in crude P.tuberosa The proposed method in precise, sensitive, reproducible and easy to perform which can be used for detection, monitoring and quantification of puerarin in P . tuberose. Medknow Publications & Media Pvt Ltd 2004 /pmc/articles/PMC3330973/ /pubmed/22557128 Text en Copyright: © Ancient Science of Life http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Chauhan, Sateesh Kumar
Singh, Brijpal
Agrawal, Sudhakar
DETERMINATION OF PUERARIN FROM PUERARIA TUBEROSA DC BY HPLC
title DETERMINATION OF PUERARIN FROM PUERARIA TUBEROSA DC BY HPLC
title_full DETERMINATION OF PUERARIN FROM PUERARIA TUBEROSA DC BY HPLC
title_fullStr DETERMINATION OF PUERARIN FROM PUERARIA TUBEROSA DC BY HPLC
title_full_unstemmed DETERMINATION OF PUERARIN FROM PUERARIA TUBEROSA DC BY HPLC
title_short DETERMINATION OF PUERARIN FROM PUERARIA TUBEROSA DC BY HPLC
title_sort determination of puerarin from pueraria tuberosa dc by hplc
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3330973/
https://www.ncbi.nlm.nih.gov/pubmed/22557128
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