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EFFECT OF L-DOPA AND L- METHIONINE SUPPLEMENTATION ON BIOPRODUCTION OF EMENTINE IN CALLUS CULTURES OF CEPHAELIS IPECACUANHA

Callus cultures were induced from leaf segments of Cephaelis ipecacuanha on modified gamborg medium containing 2,4 – dichlorophenoxy acetic acid (2mg/1) Indole acetic acid (1mg/1), kinetin (1mg/1) and sucrose (20g/1). The biogenetic profile for alkaloids was established. The established callus cultu...

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Detalles Bibliográficos
Autores principales: Vetrichelvan, T., Kavimani, S., Elango, R., Jaykar, B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 1996
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3331131/
https://www.ncbi.nlm.nih.gov/pubmed/22556774
Descripción
Sumario:Callus cultures were induced from leaf segments of Cephaelis ipecacuanha on modified gamborg medium containing 2,4 – dichlorophenoxy acetic acid (2mg/1) Indole acetic acid (1mg/1), kinetin (1mg/1) and sucrose (20g/1). The biogenetic profile for alkaloids was established. The established callus cultures were transferred to modified gamborg medium supplemented with L- DOPA (40 mg/1) and L methionine (3mg/1). After four weeks of their growth, the biomass and medium were extracted for emetine alkaloid and analysed by HPLC. The bioproduction of emetine was found to be extracellular in nature. The emetine content had increased to 0.587% mg FW as compared to control cultured (0.0245% mg FW).