EFFECT OF L-DOPA AND L- METHIONINE SUPPLEMENTATION ON BIOPRODUCTION OF EMENTINE IN CALLUS CULTURES OF CEPHAELIS IPECACUANHA

Callus cultures were induced from leaf segments of Cephaelis ipecacuanha on modified gamborg medium containing 2,4 – dichlorophenoxy acetic acid (2mg/1) Indole acetic acid (1mg/1), kinetin (1mg/1) and sucrose (20g/1). The biogenetic profile for alkaloids was established. The established callus cultu...

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Detalles Bibliográficos
Autores principales: Vetrichelvan, T., Kavimani, S., Elango, R., Jaykar, B.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Medknow Publications & Media Pvt Ltd 1996
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3331131/
https://www.ncbi.nlm.nih.gov/pubmed/22556774
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author Vetrichelvan, T.
Kavimani, S.
Elango, R.
Jaykar, B.
author_facet Vetrichelvan, T.
Kavimani, S.
Elango, R.
Jaykar, B.
author_sort Vetrichelvan, T.
collection PubMed
description Callus cultures were induced from leaf segments of Cephaelis ipecacuanha on modified gamborg medium containing 2,4 – dichlorophenoxy acetic acid (2mg/1) Indole acetic acid (1mg/1), kinetin (1mg/1) and sucrose (20g/1). The biogenetic profile for alkaloids was established. The established callus cultures were transferred to modified gamborg medium supplemented with L- DOPA (40 mg/1) and L methionine (3mg/1). After four weeks of their growth, the biomass and medium were extracted for emetine alkaloid and analysed by HPLC. The bioproduction of emetine was found to be extracellular in nature. The emetine content had increased to 0.587% mg FW as compared to control cultured (0.0245% mg FW).
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spelling pubmed-33311312012-04-27 EFFECT OF L-DOPA AND L- METHIONINE SUPPLEMENTATION ON BIOPRODUCTION OF EMENTINE IN CALLUS CULTURES OF CEPHAELIS IPECACUANHA Vetrichelvan, T. Kavimani, S. Elango, R. Jaykar, B. Anc Sci Life Original Article Callus cultures were induced from leaf segments of Cephaelis ipecacuanha on modified gamborg medium containing 2,4 – dichlorophenoxy acetic acid (2mg/1) Indole acetic acid (1mg/1), kinetin (1mg/1) and sucrose (20g/1). The biogenetic profile for alkaloids was established. The established callus cultures were transferred to modified gamborg medium supplemented with L- DOPA (40 mg/1) and L methionine (3mg/1). After four weeks of their growth, the biomass and medium were extracted for emetine alkaloid and analysed by HPLC. The bioproduction of emetine was found to be extracellular in nature. The emetine content had increased to 0.587% mg FW as compared to control cultured (0.0245% mg FW). Medknow Publications & Media Pvt Ltd 1996 /pmc/articles/PMC3331131/ /pubmed/22556774 Text en Copyright: © Ancient Science of Life http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Vetrichelvan, T.
Kavimani, S.
Elango, R.
Jaykar, B.
EFFECT OF L-DOPA AND L- METHIONINE SUPPLEMENTATION ON BIOPRODUCTION OF EMENTINE IN CALLUS CULTURES OF CEPHAELIS IPECACUANHA
title EFFECT OF L-DOPA AND L- METHIONINE SUPPLEMENTATION ON BIOPRODUCTION OF EMENTINE IN CALLUS CULTURES OF CEPHAELIS IPECACUANHA
title_full EFFECT OF L-DOPA AND L- METHIONINE SUPPLEMENTATION ON BIOPRODUCTION OF EMENTINE IN CALLUS CULTURES OF CEPHAELIS IPECACUANHA
title_fullStr EFFECT OF L-DOPA AND L- METHIONINE SUPPLEMENTATION ON BIOPRODUCTION OF EMENTINE IN CALLUS CULTURES OF CEPHAELIS IPECACUANHA
title_full_unstemmed EFFECT OF L-DOPA AND L- METHIONINE SUPPLEMENTATION ON BIOPRODUCTION OF EMENTINE IN CALLUS CULTURES OF CEPHAELIS IPECACUANHA
title_short EFFECT OF L-DOPA AND L- METHIONINE SUPPLEMENTATION ON BIOPRODUCTION OF EMENTINE IN CALLUS CULTURES OF CEPHAELIS IPECACUANHA
title_sort effect of l-dopa and l- methionine supplementation on bioproduction of ementine in callus cultures of cephaelis ipecacuanha
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3331131/
https://www.ncbi.nlm.nih.gov/pubmed/22556774
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