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Clinical Usefulness of Multiplex PCR Lateral Flow in MRSA Detection: A Novel, Rapid Genetic Testing Method

Methicillin-resistant Staphylococcus aureus (MRSA) with exogenous cassette DNA containing the methicillin-resistant gene mecA (SCCmec) poses a problem as a drug-resistant bacterium responsible for hospital- and community-acquired infections. The frequency of MRSA detection has recently been increasi...

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Autores principales: Nihonyanagi, Shin, Kanoh, Yuhsaku, Okada, Kiyomi, Uozumi, Toshiki, Kazuyama, Yukumasa, Yamaguchi, Tokiko, Nakazaki, Nobuhiko, Sakurai, Keizou, Hirata, Yasuyoshi, Munekata, Shinichi, Ohtani, Shinichi, Takemoto, Tsuyoshi, Bandoh, Yuki, Akahoshi, Tohru
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2011
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3332352/
https://www.ncbi.nlm.nih.gov/pubmed/21994180
http://dx.doi.org/10.1007/s10753-011-9395-4
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author Nihonyanagi, Shin
Kanoh, Yuhsaku
Okada, Kiyomi
Uozumi, Toshiki
Kazuyama, Yukumasa
Yamaguchi, Tokiko
Nakazaki, Nobuhiko
Sakurai, Keizou
Hirata, Yasuyoshi
Munekata, Shinichi
Ohtani, Shinichi
Takemoto, Tsuyoshi
Bandoh, Yuki
Akahoshi, Tohru
author_facet Nihonyanagi, Shin
Kanoh, Yuhsaku
Okada, Kiyomi
Uozumi, Toshiki
Kazuyama, Yukumasa
Yamaguchi, Tokiko
Nakazaki, Nobuhiko
Sakurai, Keizou
Hirata, Yasuyoshi
Munekata, Shinichi
Ohtani, Shinichi
Takemoto, Tsuyoshi
Bandoh, Yuki
Akahoshi, Tohru
author_sort Nihonyanagi, Shin
collection PubMed
description Methicillin-resistant Staphylococcus aureus (MRSA) with exogenous cassette DNA containing the methicillin-resistant gene mecA (SCCmec) poses a problem as a drug-resistant bacterium responsible for hospital- and community-acquired infections. The frequency of MRSA detection has recently been increasing rapidly in Japan, and SCCmec has also been classified more diversely into types I–V. A rapid test is essential for early diagnosis and treatment of MRSA infections, but detection by conventional methods requires at least two days. The newly developed multiplex PCR lateral flow method allows specific amplification of femA to detect S. aureus, mecA to detect SCCmec, and kdpC to detect SCCmec type II; moreover, PCR products can be evaluated visually in about 3 h. In the present study, we developed a PCR lateral flow method for MRSA using this method and investigated its clinical usefulness in the detection of MRSA. The results showed a diagnostic concordance rate of 91.7% for MRSA and methicillin-susceptible S. aureus between bacteriological examination and PCR lateral flow, and a high level of specificity in PCR lateral flow. In addition, a higher detection rate for S. aureus using the same sample was observed for PCR lateral flow (70.2%) than for bacteriological tests (48.6%). The above results show that PCR lateral flow for MRSA detection has high sensitivity, specificity, and speed, and its clinical application as a method for early diagnosis of MRSA infections appears to be feasible.
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spelling pubmed-33323522012-05-14 Clinical Usefulness of Multiplex PCR Lateral Flow in MRSA Detection: A Novel, Rapid Genetic Testing Method Nihonyanagi, Shin Kanoh, Yuhsaku Okada, Kiyomi Uozumi, Toshiki Kazuyama, Yukumasa Yamaguchi, Tokiko Nakazaki, Nobuhiko Sakurai, Keizou Hirata, Yasuyoshi Munekata, Shinichi Ohtani, Shinichi Takemoto, Tsuyoshi Bandoh, Yuki Akahoshi, Tohru Inflammation Article Methicillin-resistant Staphylococcus aureus (MRSA) with exogenous cassette DNA containing the methicillin-resistant gene mecA (SCCmec) poses a problem as a drug-resistant bacterium responsible for hospital- and community-acquired infections. The frequency of MRSA detection has recently been increasing rapidly in Japan, and SCCmec has also been classified more diversely into types I–V. A rapid test is essential for early diagnosis and treatment of MRSA infections, but detection by conventional methods requires at least two days. The newly developed multiplex PCR lateral flow method allows specific amplification of femA to detect S. aureus, mecA to detect SCCmec, and kdpC to detect SCCmec type II; moreover, PCR products can be evaluated visually in about 3 h. In the present study, we developed a PCR lateral flow method for MRSA using this method and investigated its clinical usefulness in the detection of MRSA. The results showed a diagnostic concordance rate of 91.7% for MRSA and methicillin-susceptible S. aureus between bacteriological examination and PCR lateral flow, and a high level of specificity in PCR lateral flow. In addition, a higher detection rate for S. aureus using the same sample was observed for PCR lateral flow (70.2%) than for bacteriological tests (48.6%). The above results show that PCR lateral flow for MRSA detection has high sensitivity, specificity, and speed, and its clinical application as a method for early diagnosis of MRSA infections appears to be feasible. Springer US 2011-10-13 2012 /pmc/articles/PMC3332352/ /pubmed/21994180 http://dx.doi.org/10.1007/s10753-011-9395-4 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Nihonyanagi, Shin
Kanoh, Yuhsaku
Okada, Kiyomi
Uozumi, Toshiki
Kazuyama, Yukumasa
Yamaguchi, Tokiko
Nakazaki, Nobuhiko
Sakurai, Keizou
Hirata, Yasuyoshi
Munekata, Shinichi
Ohtani, Shinichi
Takemoto, Tsuyoshi
Bandoh, Yuki
Akahoshi, Tohru
Clinical Usefulness of Multiplex PCR Lateral Flow in MRSA Detection: A Novel, Rapid Genetic Testing Method
title Clinical Usefulness of Multiplex PCR Lateral Flow in MRSA Detection: A Novel, Rapid Genetic Testing Method
title_full Clinical Usefulness of Multiplex PCR Lateral Flow in MRSA Detection: A Novel, Rapid Genetic Testing Method
title_fullStr Clinical Usefulness of Multiplex PCR Lateral Flow in MRSA Detection: A Novel, Rapid Genetic Testing Method
title_full_unstemmed Clinical Usefulness of Multiplex PCR Lateral Flow in MRSA Detection: A Novel, Rapid Genetic Testing Method
title_short Clinical Usefulness of Multiplex PCR Lateral Flow in MRSA Detection: A Novel, Rapid Genetic Testing Method
title_sort clinical usefulness of multiplex pcr lateral flow in mrsa detection: a novel, rapid genetic testing method
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3332352/
https://www.ncbi.nlm.nih.gov/pubmed/21994180
http://dx.doi.org/10.1007/s10753-011-9395-4
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