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Clinical Usefulness of Multiplex PCR Lateral Flow in MRSA Detection: A Novel, Rapid Genetic Testing Method
Methicillin-resistant Staphylococcus aureus (MRSA) with exogenous cassette DNA containing the methicillin-resistant gene mecA (SCCmec) poses a problem as a drug-resistant bacterium responsible for hospital- and community-acquired infections. The frequency of MRSA detection has recently been increasi...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3332352/ https://www.ncbi.nlm.nih.gov/pubmed/21994180 http://dx.doi.org/10.1007/s10753-011-9395-4 |
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author | Nihonyanagi, Shin Kanoh, Yuhsaku Okada, Kiyomi Uozumi, Toshiki Kazuyama, Yukumasa Yamaguchi, Tokiko Nakazaki, Nobuhiko Sakurai, Keizou Hirata, Yasuyoshi Munekata, Shinichi Ohtani, Shinichi Takemoto, Tsuyoshi Bandoh, Yuki Akahoshi, Tohru |
author_facet | Nihonyanagi, Shin Kanoh, Yuhsaku Okada, Kiyomi Uozumi, Toshiki Kazuyama, Yukumasa Yamaguchi, Tokiko Nakazaki, Nobuhiko Sakurai, Keizou Hirata, Yasuyoshi Munekata, Shinichi Ohtani, Shinichi Takemoto, Tsuyoshi Bandoh, Yuki Akahoshi, Tohru |
author_sort | Nihonyanagi, Shin |
collection | PubMed |
description | Methicillin-resistant Staphylococcus aureus (MRSA) with exogenous cassette DNA containing the methicillin-resistant gene mecA (SCCmec) poses a problem as a drug-resistant bacterium responsible for hospital- and community-acquired infections. The frequency of MRSA detection has recently been increasing rapidly in Japan, and SCCmec has also been classified more diversely into types I–V. A rapid test is essential for early diagnosis and treatment of MRSA infections, but detection by conventional methods requires at least two days. The newly developed multiplex PCR lateral flow method allows specific amplification of femA to detect S. aureus, mecA to detect SCCmec, and kdpC to detect SCCmec type II; moreover, PCR products can be evaluated visually in about 3 h. In the present study, we developed a PCR lateral flow method for MRSA using this method and investigated its clinical usefulness in the detection of MRSA. The results showed a diagnostic concordance rate of 91.7% for MRSA and methicillin-susceptible S. aureus between bacteriological examination and PCR lateral flow, and a high level of specificity in PCR lateral flow. In addition, a higher detection rate for S. aureus using the same sample was observed for PCR lateral flow (70.2%) than for bacteriological tests (48.6%). The above results show that PCR lateral flow for MRSA detection has high sensitivity, specificity, and speed, and its clinical application as a method for early diagnosis of MRSA infections appears to be feasible. |
format | Online Article Text |
id | pubmed-3332352 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-33323522012-05-14 Clinical Usefulness of Multiplex PCR Lateral Flow in MRSA Detection: A Novel, Rapid Genetic Testing Method Nihonyanagi, Shin Kanoh, Yuhsaku Okada, Kiyomi Uozumi, Toshiki Kazuyama, Yukumasa Yamaguchi, Tokiko Nakazaki, Nobuhiko Sakurai, Keizou Hirata, Yasuyoshi Munekata, Shinichi Ohtani, Shinichi Takemoto, Tsuyoshi Bandoh, Yuki Akahoshi, Tohru Inflammation Article Methicillin-resistant Staphylococcus aureus (MRSA) with exogenous cassette DNA containing the methicillin-resistant gene mecA (SCCmec) poses a problem as a drug-resistant bacterium responsible for hospital- and community-acquired infections. The frequency of MRSA detection has recently been increasing rapidly in Japan, and SCCmec has also been classified more diversely into types I–V. A rapid test is essential for early diagnosis and treatment of MRSA infections, but detection by conventional methods requires at least two days. The newly developed multiplex PCR lateral flow method allows specific amplification of femA to detect S. aureus, mecA to detect SCCmec, and kdpC to detect SCCmec type II; moreover, PCR products can be evaluated visually in about 3 h. In the present study, we developed a PCR lateral flow method for MRSA using this method and investigated its clinical usefulness in the detection of MRSA. The results showed a diagnostic concordance rate of 91.7% for MRSA and methicillin-susceptible S. aureus between bacteriological examination and PCR lateral flow, and a high level of specificity in PCR lateral flow. In addition, a higher detection rate for S. aureus using the same sample was observed for PCR lateral flow (70.2%) than for bacteriological tests (48.6%). The above results show that PCR lateral flow for MRSA detection has high sensitivity, specificity, and speed, and its clinical application as a method for early diagnosis of MRSA infections appears to be feasible. Springer US 2011-10-13 2012 /pmc/articles/PMC3332352/ /pubmed/21994180 http://dx.doi.org/10.1007/s10753-011-9395-4 Text en © The Author(s) 2011 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited. |
spellingShingle | Article Nihonyanagi, Shin Kanoh, Yuhsaku Okada, Kiyomi Uozumi, Toshiki Kazuyama, Yukumasa Yamaguchi, Tokiko Nakazaki, Nobuhiko Sakurai, Keizou Hirata, Yasuyoshi Munekata, Shinichi Ohtani, Shinichi Takemoto, Tsuyoshi Bandoh, Yuki Akahoshi, Tohru Clinical Usefulness of Multiplex PCR Lateral Flow in MRSA Detection: A Novel, Rapid Genetic Testing Method |
title | Clinical Usefulness of Multiplex PCR Lateral Flow in MRSA Detection: A Novel, Rapid Genetic Testing Method |
title_full | Clinical Usefulness of Multiplex PCR Lateral Flow in MRSA Detection: A Novel, Rapid Genetic Testing Method |
title_fullStr | Clinical Usefulness of Multiplex PCR Lateral Flow in MRSA Detection: A Novel, Rapid Genetic Testing Method |
title_full_unstemmed | Clinical Usefulness of Multiplex PCR Lateral Flow in MRSA Detection: A Novel, Rapid Genetic Testing Method |
title_short | Clinical Usefulness of Multiplex PCR Lateral Flow in MRSA Detection: A Novel, Rapid Genetic Testing Method |
title_sort | clinical usefulness of multiplex pcr lateral flow in mrsa detection: a novel, rapid genetic testing method |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3332352/ https://www.ncbi.nlm.nih.gov/pubmed/21994180 http://dx.doi.org/10.1007/s10753-011-9395-4 |
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