Cargando…

Ubiquitous urease affects soybean susceptibility to fungi

The soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling metabolically derived urea. Additional biological roles have been demonstrated for plant ureases, notably in toxicity to other organisms. However, urease enzymatic activity is not related to its toxicity. The role of GmEu4...

Descripción completa

Detalles Bibliográficos
Autores principales: Wiebke-Strohm, Beatriz, Pasquali, Giancarlo, Margis-Pinheiro, Márcia, Bencke, Marta, Bücker-Neto, Lauro, Becker-Ritt, Arlete B., Martinelli, Anne H. S., Rechenmacher, Ciliana, Polacco, Joseph C., Stolf, Renata, Marcelino, Francismar C., Abdelnoor, Ricardo V., Homrich, Milena S., Del Ponte, Emerson M., Carlini, Celia R., De Carvalho, Mayra C. C. G., Bodanese-Zanettini, Maria Helena
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3332383/
https://www.ncbi.nlm.nih.gov/pubmed/22382992
http://dx.doi.org/10.1007/s11103-012-9894-1
_version_ 1782230218005544960
author Wiebke-Strohm, Beatriz
Pasquali, Giancarlo
Margis-Pinheiro, Márcia
Bencke, Marta
Bücker-Neto, Lauro
Becker-Ritt, Arlete B.
Martinelli, Anne H. S.
Rechenmacher, Ciliana
Polacco, Joseph C.
Stolf, Renata
Marcelino, Francismar C.
Abdelnoor, Ricardo V.
Homrich, Milena S.
Del Ponte, Emerson M.
Carlini, Celia R.
De Carvalho, Mayra C. C. G.
Bodanese-Zanettini, Maria Helena
author_facet Wiebke-Strohm, Beatriz
Pasquali, Giancarlo
Margis-Pinheiro, Márcia
Bencke, Marta
Bücker-Neto, Lauro
Becker-Ritt, Arlete B.
Martinelli, Anne H. S.
Rechenmacher, Ciliana
Polacco, Joseph C.
Stolf, Renata
Marcelino, Francismar C.
Abdelnoor, Ricardo V.
Homrich, Milena S.
Del Ponte, Emerson M.
Carlini, Celia R.
De Carvalho, Mayra C. C. G.
Bodanese-Zanettini, Maria Helena
author_sort Wiebke-Strohm, Beatriz
collection PubMed
description The soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling metabolically derived urea. Additional biological roles have been demonstrated for plant ureases, notably in toxicity to other organisms. However, urease enzymatic activity is not related to its toxicity. The role of GmEu4 in soybean susceptibility to fungi was investigated in this study. A differential expression pattern of GmEu4 was observed in susceptible and resistant genotypes of soybeans over the course of a Phakopsora pachyrhizi infection, especially 24 h after infection. Twenty-nine adult, transgenic soybean plants, representing six independently transformed lines, were obtained. Although the initial aim of this study was to overexpress GmEu4, the transgenic plants exhibited GmEu4 co-suppression and decreased ureolytic activity. The growth of Rhizoctonia solani, Phomopsis sp., and Penicillium herguei in media containing a crude protein extract from either transgenic or non-transgenic leaves was evaluated. The fungal growth was higher in the protein extracts from transgenic urease-deprived plants than in extracts from non-transgenic controls. When infected by P. pachyrhizi uredospores, detached leaves of urease-deprived plants developed a significantly higher number of lesions, pustules and erupted pustules than leaves of non-transgenic plants containing normal levels of the enzyme. The results of the present work show that the soybean plants were more susceptible to fungi in the absence of urease. It was not possible to overexpress active GmEu4. For future work, overexpression of urease fungitoxic peptides could be attempted as an alternative approach. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11103-012-9894-1) contains supplementary material, which is available to authorized users.
format Online
Article
Text
id pubmed-3332383
institution National Center for Biotechnology Information
language English
publishDate 2012
publisher Springer Netherlands
record_format MEDLINE/PubMed
spelling pubmed-33323832012-05-14 Ubiquitous urease affects soybean susceptibility to fungi Wiebke-Strohm, Beatriz Pasquali, Giancarlo Margis-Pinheiro, Márcia Bencke, Marta Bücker-Neto, Lauro Becker-Ritt, Arlete B. Martinelli, Anne H. S. Rechenmacher, Ciliana Polacco, Joseph C. Stolf, Renata Marcelino, Francismar C. Abdelnoor, Ricardo V. Homrich, Milena S. Del Ponte, Emerson M. Carlini, Celia R. De Carvalho, Mayra C. C. G. Bodanese-Zanettini, Maria Helena Plant Mol Biol Article The soybean ubiquitous urease (encoded by GmEu4) is responsible for recycling metabolically derived urea. Additional biological roles have been demonstrated for plant ureases, notably in toxicity to other organisms. However, urease enzymatic activity is not related to its toxicity. The role of GmEu4 in soybean susceptibility to fungi was investigated in this study. A differential expression pattern of GmEu4 was observed in susceptible and resistant genotypes of soybeans over the course of a Phakopsora pachyrhizi infection, especially 24 h after infection. Twenty-nine adult, transgenic soybean plants, representing six independently transformed lines, were obtained. Although the initial aim of this study was to overexpress GmEu4, the transgenic plants exhibited GmEu4 co-suppression and decreased ureolytic activity. The growth of Rhizoctonia solani, Phomopsis sp., and Penicillium herguei in media containing a crude protein extract from either transgenic or non-transgenic leaves was evaluated. The fungal growth was higher in the protein extracts from transgenic urease-deprived plants than in extracts from non-transgenic controls. When infected by P. pachyrhizi uredospores, detached leaves of urease-deprived plants developed a significantly higher number of lesions, pustules and erupted pustules than leaves of non-transgenic plants containing normal levels of the enzyme. The results of the present work show that the soybean plants were more susceptible to fungi in the absence of urease. It was not possible to overexpress active GmEu4. For future work, overexpression of urease fungitoxic peptides could be attempted as an alternative approach. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11103-012-9894-1) contains supplementary material, which is available to authorized users. Springer Netherlands 2012-03-01 2012 /pmc/articles/PMC3332383/ /pubmed/22382992 http://dx.doi.org/10.1007/s11103-012-9894-1 Text en © The Author(s) 2012 https://creativecommons.org/licenses/by/4.0/ This article is distributed under the terms of the Creative Commons Attribution License which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited.
spellingShingle Article
Wiebke-Strohm, Beatriz
Pasquali, Giancarlo
Margis-Pinheiro, Márcia
Bencke, Marta
Bücker-Neto, Lauro
Becker-Ritt, Arlete B.
Martinelli, Anne H. S.
Rechenmacher, Ciliana
Polacco, Joseph C.
Stolf, Renata
Marcelino, Francismar C.
Abdelnoor, Ricardo V.
Homrich, Milena S.
Del Ponte, Emerson M.
Carlini, Celia R.
De Carvalho, Mayra C. C. G.
Bodanese-Zanettini, Maria Helena
Ubiquitous urease affects soybean susceptibility to fungi
title Ubiquitous urease affects soybean susceptibility to fungi
title_full Ubiquitous urease affects soybean susceptibility to fungi
title_fullStr Ubiquitous urease affects soybean susceptibility to fungi
title_full_unstemmed Ubiquitous urease affects soybean susceptibility to fungi
title_short Ubiquitous urease affects soybean susceptibility to fungi
title_sort ubiquitous urease affects soybean susceptibility to fungi
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3332383/
https://www.ncbi.nlm.nih.gov/pubmed/22382992
http://dx.doi.org/10.1007/s11103-012-9894-1
work_keys_str_mv AT wiebkestrohmbeatriz ubiquitousureaseaffectssoybeansusceptibilitytofungi
AT pasqualigiancarlo ubiquitousureaseaffectssoybeansusceptibilitytofungi
AT margispinheiromarcia ubiquitousureaseaffectssoybeansusceptibilitytofungi
AT benckemarta ubiquitousureaseaffectssoybeansusceptibilitytofungi
AT buckernetolauro ubiquitousureaseaffectssoybeansusceptibilitytofungi
AT beckerrittarleteb ubiquitousureaseaffectssoybeansusceptibilitytofungi
AT martinelliannehs ubiquitousureaseaffectssoybeansusceptibilitytofungi
AT rechenmacherciliana ubiquitousureaseaffectssoybeansusceptibilitytofungi
AT polaccojosephc ubiquitousureaseaffectssoybeansusceptibilitytofungi
AT stolfrenata ubiquitousureaseaffectssoybeansusceptibilitytofungi
AT marcelinofrancismarc ubiquitousureaseaffectssoybeansusceptibilitytofungi
AT abdelnoorricardov ubiquitousureaseaffectssoybeansusceptibilitytofungi
AT homrichmilenas ubiquitousureaseaffectssoybeansusceptibilitytofungi
AT delponteemersonm ubiquitousureaseaffectssoybeansusceptibilitytofungi
AT carliniceliar ubiquitousureaseaffectssoybeansusceptibilitytofungi
AT decarvalhomayraccg ubiquitousureaseaffectssoybeansusceptibilitytofungi
AT bodanesezanettinimariahelena ubiquitousureaseaffectssoybeansusceptibilitytofungi