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Human box C/D snoRNA processing conservation across multiple cell types
Small nucleolar RNAs (snoRNAs) function mainly as guides for the post-transcriptional modification of ribosomal RNAs (rRNAs). In recent years, several studies have identified a wealth of small fragments (<35 nt) derived from snoRNAs (termed sdRNAs) that stably accumulate in the cell, some of whic...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2012
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3333852/ https://www.ncbi.nlm.nih.gov/pubmed/22199253 http://dx.doi.org/10.1093/nar/gkr1233 |
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author | Scott, Michelle S. Ono, Motoharu Yamada, Kayo Endo, Akinori Barton, Geoffrey J. Lamond, Angus I. |
author_facet | Scott, Michelle S. Ono, Motoharu Yamada, Kayo Endo, Akinori Barton, Geoffrey J. Lamond, Angus I. |
author_sort | Scott, Michelle S. |
collection | PubMed |
description | Small nucleolar RNAs (snoRNAs) function mainly as guides for the post-transcriptional modification of ribosomal RNAs (rRNAs). In recent years, several studies have identified a wealth of small fragments (<35 nt) derived from snoRNAs (termed sdRNAs) that stably accumulate in the cell, some of which may regulate splicing or translation. A comparison of human small RNA deep sequencing data sets reveals that box C/D sdRNA accumulation patterns are conserved across multiple cell types although the ratio of the abundance of different sdRNAs from a given snoRNA varies. sdRNA profiles of many snoRNAs are specific and resemble the cleavage profiles of miRNAs. Many do not show characteristics of general RNA degradation, as seen for the accumulation of small fragments derived from snRNA or rRNA. While 53% of the sdRNAs contain an snoRNA box C motif and boxes D and D′ are also common in sdRNAs (54%), relatively few (12%) contain a full snoRNA guide region. One box C/D snoRNA, HBII-180C, was analysed in greater detail, revealing the presence of C′ box-containing sdRNAs complementary to several pre-messenger RNAs (pre-mRNAs) including FGFR3. Functional analyses demonstrated that this region of HBII-180C can influence the alternative splicing of FGFR3 pre-mRNA, supporting a role for some snoRNAs in the regulation of splicing. |
format | Online Article Text |
id | pubmed-3333852 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2012 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-33338522012-04-23 Human box C/D snoRNA processing conservation across multiple cell types Scott, Michelle S. Ono, Motoharu Yamada, Kayo Endo, Akinori Barton, Geoffrey J. Lamond, Angus I. Nucleic Acids Res RNA Small nucleolar RNAs (snoRNAs) function mainly as guides for the post-transcriptional modification of ribosomal RNAs (rRNAs). In recent years, several studies have identified a wealth of small fragments (<35 nt) derived from snoRNAs (termed sdRNAs) that stably accumulate in the cell, some of which may regulate splicing or translation. A comparison of human small RNA deep sequencing data sets reveals that box C/D sdRNA accumulation patterns are conserved across multiple cell types although the ratio of the abundance of different sdRNAs from a given snoRNA varies. sdRNA profiles of many snoRNAs are specific and resemble the cleavage profiles of miRNAs. Many do not show characteristics of general RNA degradation, as seen for the accumulation of small fragments derived from snRNA or rRNA. While 53% of the sdRNAs contain an snoRNA box C motif and boxes D and D′ are also common in sdRNAs (54%), relatively few (12%) contain a full snoRNA guide region. One box C/D snoRNA, HBII-180C, was analysed in greater detail, revealing the presence of C′ box-containing sdRNAs complementary to several pre-messenger RNAs (pre-mRNAs) including FGFR3. Functional analyses demonstrated that this region of HBII-180C can influence the alternative splicing of FGFR3 pre-mRNA, supporting a role for some snoRNAs in the regulation of splicing. Oxford University Press 2012-04 2011-12-22 /pmc/articles/PMC3333852/ /pubmed/22199253 http://dx.doi.org/10.1093/nar/gkr1233 Text en © The Author(s) 2011. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | RNA Scott, Michelle S. Ono, Motoharu Yamada, Kayo Endo, Akinori Barton, Geoffrey J. Lamond, Angus I. Human box C/D snoRNA processing conservation across multiple cell types |
title | Human box C/D snoRNA processing conservation across multiple cell types |
title_full | Human box C/D snoRNA processing conservation across multiple cell types |
title_fullStr | Human box C/D snoRNA processing conservation across multiple cell types |
title_full_unstemmed | Human box C/D snoRNA processing conservation across multiple cell types |
title_short | Human box C/D snoRNA processing conservation across multiple cell types |
title_sort | human box c/d snorna processing conservation across multiple cell types |
topic | RNA |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3333852/ https://www.ncbi.nlm.nih.gov/pubmed/22199253 http://dx.doi.org/10.1093/nar/gkr1233 |
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