SLiCE: a novel bacterial cell extract-based DNA cloning method

We describe a novel cloning method termed SLiCE (Seamless Ligation Cloning Extract) that utilizes easy to generate bacterial cell extracts to assemble multiple DNA fragments into recombinant DNA molecules in a single in vitro recombination reaction. SLiCE overcomes the sequence limitations of tradit...

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Detalles Bibliográficos
Autores principales: Zhang, Yongwei, Werling, Uwe, Edelmann, Winfried
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3333860/
https://www.ncbi.nlm.nih.gov/pubmed/22241772
http://dx.doi.org/10.1093/nar/gkr1288

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3333860/
https://www.ncbi.nlm.nih.gov/pubmed/22241772
http://dx.doi.org/10.1093/nar/gkr1288

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