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SLiCE: a novel bacterial cell extract-based DNA cloning method

We describe a novel cloning method termed SLiCE (Seamless Ligation Cloning Extract) that utilizes easy to generate bacterial cell extracts to assemble multiple DNA fragments into recombinant DNA molecules in a single in vitro recombination reaction. SLiCE overcomes the sequence limitations of tradit...

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Detalles Bibliográficos
Autores principales:	Zhang, Yongwei, Werling, Uwe, Edelmann, Winfried
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2012
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3333860/ https://www.ncbi.nlm.nih.gov/pubmed/22241772 http://dx.doi.org/10.1093/nar/gkr1288

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