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Yin Yang 1 extends the Myc-related transcription factors network in embryonic stem cells

The Yin Yang 1 (YY1) transcription factor is a master regulator of development, essential for early embryogenesis and adult tissues formation. YY1 is the mammalian orthologue of Pleiohomeotic, one of the transcription factors that binds Polycomb DNA response elements in Drosophila melanogaster and m...

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Autores principales: Vella, Pietro, Barozzi, Iros, Cuomo, Alessandro, Bonaldi, Tiziana, Pasini, Diego
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3333890/
https://www.ncbi.nlm.nih.gov/pubmed/22210892
http://dx.doi.org/10.1093/nar/gkr1290
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author Vella, Pietro
Barozzi, Iros
Cuomo, Alessandro
Bonaldi, Tiziana
Pasini, Diego
author_facet Vella, Pietro
Barozzi, Iros
Cuomo, Alessandro
Bonaldi, Tiziana
Pasini, Diego
author_sort Vella, Pietro
collection PubMed
description The Yin Yang 1 (YY1) transcription factor is a master regulator of development, essential for early embryogenesis and adult tissues formation. YY1 is the mammalian orthologue of Pleiohomeotic, one of the transcription factors that binds Polycomb DNA response elements in Drosophila melanogaster and mediates Polycomb group proteins (PcG) recruitment to DNA. Despite several publications pointing at YY1 having a similar role in mammalians, others showed features of YY1 that are not compatible with PcG functions. Here, we show that, in mouse Embryonic Stem (ES) cells, YY1 has genome-wide PcG-independent activities while it is still stably associated with the INO80 chromatin-remodeling complex, as well as with novel RNA helicase activities. YY1 binds chromatin in close proximity of the transcription start site of highly expressed genes. Loss of YY1 functions preferentially led to a down-regulation of target genes expression, as well as to an up-regulation of several small non-coding RNAs, suggesting a role for YY1 in regulating small RNA biogenesis. Finally, we found that YY1 is a novel player of Myc-related transcription factors and that its coordinated binding at promoters potentiates gene expression, proposing YY1 as an active component of the Myc transcription network that links ES to cancer cells.
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spelling pubmed-33338902012-04-23 Yin Yang 1 extends the Myc-related transcription factors network in embryonic stem cells Vella, Pietro Barozzi, Iros Cuomo, Alessandro Bonaldi, Tiziana Pasini, Diego Nucleic Acids Res Gene Regulation, Chromatin and Epigenetics The Yin Yang 1 (YY1) transcription factor is a master regulator of development, essential for early embryogenesis and adult tissues formation. YY1 is the mammalian orthologue of Pleiohomeotic, one of the transcription factors that binds Polycomb DNA response elements in Drosophila melanogaster and mediates Polycomb group proteins (PcG) recruitment to DNA. Despite several publications pointing at YY1 having a similar role in mammalians, others showed features of YY1 that are not compatible with PcG functions. Here, we show that, in mouse Embryonic Stem (ES) cells, YY1 has genome-wide PcG-independent activities while it is still stably associated with the INO80 chromatin-remodeling complex, as well as with novel RNA helicase activities. YY1 binds chromatin in close proximity of the transcription start site of highly expressed genes. Loss of YY1 functions preferentially led to a down-regulation of target genes expression, as well as to an up-regulation of several small non-coding RNAs, suggesting a role for YY1 in regulating small RNA biogenesis. Finally, we found that YY1 is a novel player of Myc-related transcription factors and that its coordinated binding at promoters potentiates gene expression, proposing YY1 as an active component of the Myc transcription network that links ES to cancer cells. Oxford University Press 2012-04 2011-12-30 /pmc/articles/PMC3333890/ /pubmed/22210892 http://dx.doi.org/10.1093/nar/gkr1290 Text en © The Author(s) 2011. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Gene Regulation, Chromatin and Epigenetics
Vella, Pietro
Barozzi, Iros
Cuomo, Alessandro
Bonaldi, Tiziana
Pasini, Diego
Yin Yang 1 extends the Myc-related transcription factors network in embryonic stem cells
title Yin Yang 1 extends the Myc-related transcription factors network in embryonic stem cells
title_full Yin Yang 1 extends the Myc-related transcription factors network in embryonic stem cells
title_fullStr Yin Yang 1 extends the Myc-related transcription factors network in embryonic stem cells
title_full_unstemmed Yin Yang 1 extends the Myc-related transcription factors network in embryonic stem cells
title_short Yin Yang 1 extends the Myc-related transcription factors network in embryonic stem cells
title_sort yin yang 1 extends the myc-related transcription factors network in embryonic stem cells
topic Gene Regulation, Chromatin and Epigenetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3333890/
https://www.ncbi.nlm.nih.gov/pubmed/22210892
http://dx.doi.org/10.1093/nar/gkr1290
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