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Hematopoietic stem cells, hematopoiesis and disease: lessons from the zebrafish model
The zebrafish model is rapidly gaining prominence in the study of development, hematopoiesis, and disease. The zebrafish provides distinct advantages over other vertebrate models during early embryonic development by producing transparent, externally fertilized embryos. Embryonic zebrafish are easil...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2011
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334548/ https://www.ncbi.nlm.nih.gov/pubmed/22206610 http://dx.doi.org/10.1186/gm299 |
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author | Martin, Corey S Moriyama, Akemi Zon, Leonard I |
author_facet | Martin, Corey S Moriyama, Akemi Zon, Leonard I |
author_sort | Martin, Corey S |
collection | PubMed |
description | The zebrafish model is rapidly gaining prominence in the study of development, hematopoiesis, and disease. The zebrafish provides distinct advantages over other vertebrate models during early embryonic development by producing transparent, externally fertilized embryos. Embryonic zebrafish are easily visualized and manipulated through microinjection, chemical treatment, and mutagenesis. These procedures have contributed to large-scale chemical, suppressor, and genetic screens to identify hematopoietic gene mutations. Genomic conservation and local synteny between the human and zebrafish genomes make genome-scale and epigenetic analysis of these mutations (by microarray, chromatin immunoprecipitation sequencing, and RNA sequencing procedures) powerful methods for translational research and medical discovery. In addition, large-scale screening techniques have resulted in the identification of several small molecules capable of rescuing hematopoietic defects and inhibiting disease. Here, we discuss the contributions of the zebrafish model to the understanding of hematopoiesis, hematopoietic stem cell development, and disease-related discovery. We also highlight the recent discovery of small molecules with clinical promise, such as dimethyl prostaglandin E2, 3F8, and thiazole-carboxamide 10A. |
format | Online Article Text |
id | pubmed-3334548 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2011 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-33345482012-12-29 Hematopoietic stem cells, hematopoiesis and disease: lessons from the zebrafish model Martin, Corey S Moriyama, Akemi Zon, Leonard I Genome Med Review The zebrafish model is rapidly gaining prominence in the study of development, hematopoiesis, and disease. The zebrafish provides distinct advantages over other vertebrate models during early embryonic development by producing transparent, externally fertilized embryos. Embryonic zebrafish are easily visualized and manipulated through microinjection, chemical treatment, and mutagenesis. These procedures have contributed to large-scale chemical, suppressor, and genetic screens to identify hematopoietic gene mutations. Genomic conservation and local synteny between the human and zebrafish genomes make genome-scale and epigenetic analysis of these mutations (by microarray, chromatin immunoprecipitation sequencing, and RNA sequencing procedures) powerful methods for translational research and medical discovery. In addition, large-scale screening techniques have resulted in the identification of several small molecules capable of rescuing hematopoietic defects and inhibiting disease. Here, we discuss the contributions of the zebrafish model to the understanding of hematopoiesis, hematopoietic stem cell development, and disease-related discovery. We also highlight the recent discovery of small molecules with clinical promise, such as dimethyl prostaglandin E2, 3F8, and thiazole-carboxamide 10A. BioMed Central 2011-12-29 /pmc/articles/PMC3334548/ /pubmed/22206610 http://dx.doi.org/10.1186/gm299 Text en Copyright ©2011 BioMed Central Ltd. |
spellingShingle | Review Martin, Corey S Moriyama, Akemi Zon, Leonard I Hematopoietic stem cells, hematopoiesis and disease: lessons from the zebrafish model |
title | Hematopoietic stem cells, hematopoiesis and disease: lessons from the zebrafish model |
title_full | Hematopoietic stem cells, hematopoiesis and disease: lessons from the zebrafish model |
title_fullStr | Hematopoietic stem cells, hematopoiesis and disease: lessons from the zebrafish model |
title_full_unstemmed | Hematopoietic stem cells, hematopoiesis and disease: lessons from the zebrafish model |
title_short | Hematopoietic stem cells, hematopoiesis and disease: lessons from the zebrafish model |
title_sort | hematopoietic stem cells, hematopoiesis and disease: lessons from the zebrafish model |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334548/ https://www.ncbi.nlm.nih.gov/pubmed/22206610 http://dx.doi.org/10.1186/gm299 |
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