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Circular Permutation in the Ω-Loop of TEM-1 β-Lactamase Results in Improved Activity and Altered Substrate Specificity

Generating diverse protein libraries that contain improved variants at a sufficiently high frequency is critical for improving the properties of proteins using directed evolution. Many studies have illustrated how random mutagenesis, cassette mutagenesis, DNA shuffling and similar approaches are eff...

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Detalles Bibliográficos
Autores principales:	Guntas, Gurkan, Kanwar, Manu, Ostermeier, Marc
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2012
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334891/ https://www.ncbi.nlm.nih.gov/pubmed/22536452 http://dx.doi.org/10.1371/journal.pone.0035998

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