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De Novo Peroxisome Biogenesis in Penicillium Chrysogenum Is Not Dependent on the Pex11 Family Members or Pex16

We have analyzed the role of the three members of the Pex11 protein family in peroxisome formation in the filamentous fungus Penicillium chrysogenum. Two of these, Pex11 and Pex11C, are components of the peroxisomal membrane, while Pex11B is present at the endoplasmic reticulum. We show that Pex11 i...

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Autores principales: Opaliński, Łukasz, Bartoszewska, Magdalena, Fekken, Susan, Liu, Haiyin, de Boer, Rinse, van der Klei, Ida, Veenhuis, Marten, Kiel, Jan A. K. W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2012
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334907/
https://www.ncbi.nlm.nih.gov/pubmed/22536392
http://dx.doi.org/10.1371/journal.pone.0035490
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author Opaliński, Łukasz
Bartoszewska, Magdalena
Fekken, Susan
Liu, Haiyin
de Boer, Rinse
van der Klei, Ida
Veenhuis, Marten
Kiel, Jan A. K. W.
author_facet Opaliński, Łukasz
Bartoszewska, Magdalena
Fekken, Susan
Liu, Haiyin
de Boer, Rinse
van der Klei, Ida
Veenhuis, Marten
Kiel, Jan A. K. W.
author_sort Opaliński, Łukasz
collection PubMed
description We have analyzed the role of the three members of the Pex11 protein family in peroxisome formation in the filamentous fungus Penicillium chrysogenum. Two of these, Pex11 and Pex11C, are components of the peroxisomal membrane, while Pex11B is present at the endoplasmic reticulum. We show that Pex11 is a major factor involved in peroxisome proliferation. We also demonstrate that P. chrysogenum cells deleted for known peroxisome fission factors (all Pex11 family proteins and Vps1) still contain peroxisomes. Interestingly, we find that, unlike in mammals, Pex16 is not essential for peroxisome biogenesis in P. chrysogenum, as partially functional peroxisomes are present in a pex16 deletion strain. We also show that Pex16 is not involved in de novo biogenesis of peroxisomes, as peroxisomes were still present in quadruple Δpex11 Δpex11B Δpex11C Δpex16 mutant cells. By contrast, pex3 deletion in P. chrysogenum led to cells devoid of peroxisomes, suggesting that Pex3 may function independently of Pex16. Finally, we demonstrate that the presence of intact peroxisomes is important for the efficiency of ß-lactam antibiotics production by P. chrysogenum. Remarkably, distinct from earlier results with low penicillin producing laboratory strains, upregulation of peroxisome numbers in a high producing P. chrysogenum strain had no significant effect on penicillin production.
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spelling pubmed-33349072012-04-25 De Novo Peroxisome Biogenesis in Penicillium Chrysogenum Is Not Dependent on the Pex11 Family Members or Pex16 Opaliński, Łukasz Bartoszewska, Magdalena Fekken, Susan Liu, Haiyin de Boer, Rinse van der Klei, Ida Veenhuis, Marten Kiel, Jan A. K. W. PLoS One Research Article We have analyzed the role of the three members of the Pex11 protein family in peroxisome formation in the filamentous fungus Penicillium chrysogenum. Two of these, Pex11 and Pex11C, are components of the peroxisomal membrane, while Pex11B is present at the endoplasmic reticulum. We show that Pex11 is a major factor involved in peroxisome proliferation. We also demonstrate that P. chrysogenum cells deleted for known peroxisome fission factors (all Pex11 family proteins and Vps1) still contain peroxisomes. Interestingly, we find that, unlike in mammals, Pex16 is not essential for peroxisome biogenesis in P. chrysogenum, as partially functional peroxisomes are present in a pex16 deletion strain. We also show that Pex16 is not involved in de novo biogenesis of peroxisomes, as peroxisomes were still present in quadruple Δpex11 Δpex11B Δpex11C Δpex16 mutant cells. By contrast, pex3 deletion in P. chrysogenum led to cells devoid of peroxisomes, suggesting that Pex3 may function independently of Pex16. Finally, we demonstrate that the presence of intact peroxisomes is important for the efficiency of ß-lactam antibiotics production by P. chrysogenum. Remarkably, distinct from earlier results with low penicillin producing laboratory strains, upregulation of peroxisome numbers in a high producing P. chrysogenum strain had no significant effect on penicillin production. Public Library of Science 2012-04-19 /pmc/articles/PMC3334907/ /pubmed/22536392 http://dx.doi.org/10.1371/journal.pone.0035490 Text en Opaliński et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Opaliński, Łukasz
Bartoszewska, Magdalena
Fekken, Susan
Liu, Haiyin
de Boer, Rinse
van der Klei, Ida
Veenhuis, Marten
Kiel, Jan A. K. W.
De Novo Peroxisome Biogenesis in Penicillium Chrysogenum Is Not Dependent on the Pex11 Family Members or Pex16
title De Novo Peroxisome Biogenesis in Penicillium Chrysogenum Is Not Dependent on the Pex11 Family Members or Pex16
title_full De Novo Peroxisome Biogenesis in Penicillium Chrysogenum Is Not Dependent on the Pex11 Family Members or Pex16
title_fullStr De Novo Peroxisome Biogenesis in Penicillium Chrysogenum Is Not Dependent on the Pex11 Family Members or Pex16
title_full_unstemmed De Novo Peroxisome Biogenesis in Penicillium Chrysogenum Is Not Dependent on the Pex11 Family Members or Pex16
title_short De Novo Peroxisome Biogenesis in Penicillium Chrysogenum Is Not Dependent on the Pex11 Family Members or Pex16
title_sort de novo peroxisome biogenesis in penicillium chrysogenum is not dependent on the pex11 family members or pex16
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3334907/
https://www.ncbi.nlm.nih.gov/pubmed/22536392
http://dx.doi.org/10.1371/journal.pone.0035490
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